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Selective use of TBP and TFIIB revealed by a TATA-TBP-TFIIB array with altered specificity.

Tansey WP, Herr W
Science. 1997 275 (5301): 829-31

PMID: 9012349 · DOI:10.1126/science.275.5301.829

Interaction between the TATA box-binding protein TBP and TFIIB is critical for transcription in vitro. An altered-specificity TBP-TFIIB interaction was rationally designed and linked in sequence to an altered-specificity TATA box-TBP interaction to study how TBP and TFIIB function together to support transcription in human cells. The activity of this altered-specificity TATA-TBP-TFIIB array demonstrated that many activators use the known TBP-TFIIB interaction to stimulate transcription. One activator, however, derived from a glutamine-rich activation domain of Sp1, activated transcription independently of this interaction. These results reveal that selectivity in activator function in vivo can be achieved through differential use of TBP and TFIIB.

MeSH Terms (14)

DNA-Binding Proteins Fungal Proteins Genes, fos Genes, Reporter HeLa Cells Humans Mutagenesis, Site-Directed Sp1 Transcription Factor TATA-Box Binding Protein TATA Box Trans-Activators Transcription, Genetic Transcription Factors Transcription Factor TFIIB

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