Freeze-thaw lysis for extraction of proteins from Mammalian cells.

Tansey WP
CSH Protoc. 2006 2006 (7)

PMID: 22484670 · DOI:10.1101/pdb.prot4614

INTRODUCTIONThis protocol describes the extraction of proteins from mammalian cells grown in 6- or 10-cm tissue-culture dishes. It is useful for making very concentrated extracts for Western blotting, and is particularly helpful when low-abundance proteins such as Myc are being studied.

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