Denaturing protein immunoprecipitation from yeast.

Tansey WP
CSH Protoc. 2007 2007: pdb.prot4643

PMID: 21356925 · DOI:10.1101/pdb.prot4643

INTRODUCTIONWhere possible, it is preferable to use denaturing IPs to recover labeled proteins from pulse-chase experiments. The use of denaturing IPs reduces background and simplifies protein harvest. Yeast are boiled in an SDS-containing solution to liberate total proteins. This is also much safer than bead-beating radioactive yeast samples.

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