Nondenaturing protein immunoprecipitation from Mammalian cells.

Tansey WP
CSH Protoc. 2007 2007: pdb.prot4620

PMID: 21356922 · DOI:10.1101/pdb.prot4620

INTRODUCTIONThis protocol describes a nondenaturing immunoprecipitation (IP) for mammalian cells. Where possible, we prefer to use denaturing IPs to recover labeled proteins from pulse-chase experiments. However, the nondenaturing protocol is useful when one wishes to separate soluble from insoluble proteins, or when the antibody being used recognizes a native epitope.

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