Denaturing protein immunoprecipitation from Mammalian cells.

Tansey WP
CSH Protoc. 2007 2007: pdb.prot4619

PMID: 21356921 · DOI:10.1101/pdb.prot4619

INTRODUCTIONThis protocol describes a denaturing immunoprecipitation (IP) for mammalian cells. Where possible, we prefer to use denaturing IPs to recover labeled proteins from pulse-chase experiments. The use of denaturing IPs reduces background considerably.

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