The Rab11-FIP1/RCP gene codes for multiple protein transcripts related to the plasma membrane recycling system.

Jin M, Goldenring JR
Biochim Biophys Acta. 2006 1759 (6): 281-95

PMID: 16920206 · DOI:10.1016/j.bbaexp.2006.06.001

Rab11a is a member of the Rab11 small GTPase family, and plays an important role in plasma membrane recycling. Rab11-Family Interacting Protein 1 (Rab11-FIP1) binds to Rab11 through a carboxyl-terminal amphipathic alpha helix. We have identified eight alternatively spliced Rab11-FIP1 gene transcripts from human chromosome 8. Among them, Rab11-FIP1A-D have carboxyl terminal Rab11 binding domains, while Rab11-FIP1E-H do not contain the Rab11 binding domain. While Rab11-FIP1B and F gene transcripts are ubiquitous, other Rab11-FIP1 transcripts demonstrate more limited patterns of expression in human tissue cDNAs. EGFP-Rab11-FIP1A-D proteins over-expressed in HeLa cells targeted to Rab11a-containing membranes, while EGFP-Rab11-FIP1E/F and H proteins did not localize with recycling system membranes. However, transferrin trafficking was not significantly altered in HeLa cells over-expressing expressing any of the EGFP-Rab11-FIP1 proteins. Rabbit polyclonal antibodies specific for Rab11-FIP1B and Rab11-FIP1C/RCP demonstrated that Rab11-FIP1B and Rab11-FIP1C/RCP are expressed endogenously. Strikingly, endogenous staining for Rab11-FIP1C/RCP only partially co-localized with EGFP-Rab11-FIP1A, EGFP-Rab11-FIP1B, and EGFP-Rab11a in the perinuclear region, indicating that Rab11-FIP1C/RCP resides in a differentiable subcellular compartment within the plasma membrane recycling system compared with Rab11-FIP1A and Rab11-FIP1B. These data suggest that Rab11-FIP1 proteins may play coordinated roles in regulating plasma membrane recycling with regional specificity within the Rab11a-containing recycling system.

MeSH Terms (31)

Adaptor Proteins, Signal Transducing Alternative Splicing Amino Acid Sequence Animals Antibody Specificity Base Sequence Binding Sites Carrier Proteins Cell Membrane Chromosomes, Human, Pair 8 Cloning, Molecular DNA, Complementary Female Gene Expression Green Fluorescent Proteins HeLa Cells Humans Male Membrane Proteins Molecular Sequence Data Open Reading Frames Pregnancy Protein Structure, Tertiary Rabbits Recombinant Fusion Proteins RNA, Messenger Sequence Homology, Amino Acid Tissue Distribution Transfection Transferrin Two-Hybrid System Techniques

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