Hak-Joon Sung
Assistant Professor of Biomedical Engineering, Assistant Professor of Medicine-Cardiovascular Medicine
Last active: 2/12/2015

High-content profiling of cell responsiveness to graded substrates based on combinyatorially variant polymers.

Liu E, Treiser MD, Patel H, Sung HJ, Roskov KE, Kohn J, Becker ML, Moghe PV
Comb Chem High Throughput Screen. 2009 12 (7): 646-55

PMID: 19531022 · PMCID: PMC3061568 · DOI:10.2174/138620709788923737

We have developed a novel approach combining high information and high throughput analysis to characterize cell adhesive responses to biomaterial substrates possessing gradients in surface topography. These gradients were fabricated by subjecting thin film blends of tyrosine-derived polycarbonates, i.e. poly(DTE carbonate) and poly(DTO carbonate) to a gradient temperature annealing protocol. Saos-2 cells engineered with a green fluorescent protein (GFP) reporter for farnesylation (GFP-f) were cultured on the gradient substrates to assess the effects of nanoscale surface topology and roughness that arise during the phase separation process on cell attachment and adhesion strength. The high throughput imaging approach allowed us to rapidly identify the "global" and "high content" structure-property relationships between cell adhesion and biomaterial properties such as polymer chemistry and topography. This study found that cell attachment and spreading increased monotonically with DTE content and were significantly elevated at the position with intermediate regions corresponding to the highest "gradient" of surface roughness, while GFP-f farnesylation intensity descriptors were sensitively altered by surface roughness, even in cells with comparable levels of spreading.

MeSH Terms (9)

Biological Assay Cell Adhesion Cell Line Combinatorial Chemistry Techniques Green Fluorescent Proteins Humans Microscopy, Atomic Force Nylons Tyrosine

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