Generation of a tenascin-C-CreER2 knockin mouse line for conditional DNA recombination in renal medullary interstitial cells.

He W, Xie Q, Wang Y, Chen J, Zhao M, Davis LS, Breyer MD, Gu G, Hao CM
PLoS One. 2013 8 (11): e79839

PMID: 24244568 · PMCID: PMC3823583 · DOI:10.1371/journal.pone.0079839

Renal medullary interstitial cells (RMIC) are specialized fibroblast-like cells that exert important functions in maintaining body fluid homeostasis and systemic blood pressure. Here, we generated a RMIC specific tenascin-C promoter driven inducible CreER2 knockin mouse line with an EGFP reporter. Similar as endogenous tenascin-C expression, the reporter EGFP expression in the tenascin-C-CreER2(+/-) mice was observed in the inner medulla of the kidney, and co-localized with COX2 but not with AQP2 or AQP1, suggesting selective expression in RMICs. After recombination (tenascin-C-CreER2(+/-)/ROSA26-lacZ(+/-) mice + tamoxifen), β-gal activity was restricted to the cells in the inner medulla of the kidney, and didn't co-localize with AQP2, consistent with selective Cre recombinase activity in RMICs. Cre activity was not obvious in other major organs or without tamoxifen treatment. This inducible RMIC specific Cre mouse line should therefore provide a novel tool to manipulate genes of interest in RMICs.

MeSH Terms (22)

Animals Aquaporin 1 Aquaporin 2 Crosses, Genetic Cyclooxygenase 2 Female Fibroblasts Founder Effect Gene Expression Regulation Gene Knock-In Techniques Genes, Reporter Green Fluorescent Proteins Integrases Kidney Medulla Lac Operon Male Mice Mice, Transgenic Promoter Regions, Genetic Tamoxifen Tenascin Transgenes

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