ETO family protein Mtg16 regulates the balance of dendritic cell subsets by repressing Id2.

Ghosh HS, Ceribelli M, Matos I, Lazarovici A, Bussemaker HJ, Lasorella A, Hiebert SW, Liu K, Staudt LM, Reizis B
J Exp Med. 2014 211 (8): 1623-35

PMID: 24980046 · PMCID: PMC4113936 · DOI:10.1084/jem.20132121

Dendritic cells (DCs) comprise two major subsets, the interferon (IFN)-producing plasmacytoid DCs (pDCs) and antigen-presenting classical DCs (cDCs). The development of pDCs is promoted by E protein transcription factor E2-2, whereas E protein antagonist Id2 is specifically absent from pDCs. Conversely, Id2 is prominently expressed in cDCs and promotes CD8(+) cDC development. The mechanisms that control the balance between E and Id proteins during DC subset specification remain unknown. We found that the loss of Mtg16, a transcriptional cofactor of the ETO protein family, profoundly impaired pDC development and pDC-dependent IFN response. The residual Mtg16-deficient pDCs showed aberrant phenotype, including the expression of myeloid marker CD11b. Conversely, the development of cDC progenitors (pre-DCs) and of CD8(+) cDCs was enhanced. Genome-wide expression and DNA-binding analysis identified Id2 as a direct target of Mtg16. Mtg16-deficient cDC progenitors and pDCs showed aberrant induction of Id2, and the deletion of Id2 facilitated the impaired development of Mtg16-deficient pDCs. Thus, Mtg16 promotes pDC differentiation and restricts cDC development in part by repressing Id2, revealing a cell-intrinsic mechanism that controls subset balance during DC development.

© 2014 Ghosh et al.

MeSH Terms (18)

Animals Base Sequence Cell Differentiation Cell Line Cell Proliferation Chromatin Dendritic Cells E2F2 Transcription Factor Gene Deletion Humans Inhibitor of Differentiation Protein 2 Mice Mice, Inbred C57BL Molecular Sequence Data Nuclear Proteins Repressor Proteins Stem Cells Transcription Factors

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