Kari Huppert
Last active: 4/4/2011

Profile

Kari A. Huppert is a Senior Research Specialist in Dr. Huppert’s laboratory, part of the Vanderbilt Center for Stem Cell Biology. In 1995, she received her B.S. in Microbiology from Purdue University. Kari has been employed in both academia and biotechnology industrial research settings. Her first research experience was at Indiana University in the laboratory of Dr. Marc A. T. Muskavitch, performing various genetic screens in Drosophila melanogaster to isolate modifiers of Delta, a ligand of the Notch pathway. This experience positioned her to lead a group at Exelixis Inc. in San Francisco, California that contributed to publicly available molecularly defined genome-wide gene disruptions within the Drosophila genome, derived from transposable elements. In addition, Kari isolated a hypomorphic allele of Drosophila Presenilin, the catalytic component of the gamma-secretase complex responsible for the intracellular cleavage and activation of Notch. Using this allele in an enhancer and suppressor screen, enabled identification of previously undescribed genes involved in extracellular matrix and vesicular trafficking of Presenilin and Notch biology. After working at Exelixis Inc. for 5 years, Kari moved back to the Midwest to work with Dr. Raphael Kopan at Washington University School of Medicine in St. Louis, Missouri. There she worked as the lab manager for 3 years. During this time Kari worked on a project to map Notch1 activation during embryonic mouse development. Kari then moved back to an industrial setting at the Donald Danforth Plant Science Center in St. Louis for 2.5 years. There she worked with Arabidopsis thaliana, maize, and grape vines. Specifically she worked on a project to determine the molecular signature of nutrient deprivation in maize by qRT-PCR. In addition, she worked on a project to define powdery mildew susceptibility in Cabernet Sauvignon. The ultimate goal of this project is to enable growth of Cabernet Sauvignon in the Midwest. In the Huppert laboratory, Kari is concentrating on defining the cell lineages that require Notch signaling to support fetal hepatoblast progenitor proliferation and liver morphogenesis.

Publications

The following timeline graph is generated from all co-authored publications.

Most recent publication(s) are shown below:

  1. Notch signaling regulates formation of the three-dimensional architecture of intrahepatic bile ducts in mice. Sparks EE, Huppert KA, Brown MA, Washington MK, Huppert SS (2010) Hepatology 51(4): 1391-400
    › Primary publication · 20069650 (PubMed) · PMC2995854 (PubMed Central)
  2. The Arabidopsis transcription factor MYB77 modulates auxin signal transduction. Shin R, Burch AY, Huppert KA, Tiwari SB, Murphy AS, Guilfoyle TJ, Schachtman DP (2007) Plant Cell 19(8): 2440-53
    › Primary publication · 17675404 (PubMed) · PMC2002618 (PubMed Central)
  3. Presenilin-based genetic screens in Drosophila melanogaster identify novel notch pathway modifiers. Mahoney MB, Parks AL, Ruddy DA, Tiong SY, Esengil H, Phan AC, Philandrinos P, Winter CG, Chatterjee R, Huppert K, Fisher WW, L'Archeveque L, Mapa FA, Woo W, Ellis MC, Curtis D (2006) Genetics 172(4): 2309-24
    › Primary publication · 16415372 (PubMed) · PMC1456381 (PubMed Central)
  4. Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. Parks AL, Cook KR, Belvin M, Dompe NA, Fawcett R, Huppert K, Tan LR, Winter CG, Bogart KP, Deal JE, Deal-Herr ME, Grant D, Marcinko M, Miyazaki WY, Robertson S, Shaw KJ, Tabios M, Vysotskaia V, Zhao L, Andrade RS, Edgar KA, Howie E, Killpack K, Milash B, Norton A, Thao D, Whittaker K, Winner MA, Friedman L, Margolis J, Singer MA, Kopczynski C, Curtis D, Kaufman TC, Plowman GD, Duyk G, Francis-Lang HL (2004) Nat Genet 36(3): 288-92
    › Primary publication · 14981519 (PubMed)