Melanie Ohi
Last active: 3/3/2020


None provided


The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Structural analysis of lecithin:cholesterol acyltransferase bound to high density lipoprotein particles. Manthei KA, Patra D, Wilson CJ, Fawaz MV, Piersimoni L, Shenkar JC, Yuan W, Andrews PC, Engen JR, Schwendeman A, Ohi MD, Tesmer JJG (2020) Commun Biol 3(1): 28
    › Primary publication · 31942029 (PubMed) · PMC6962161 (PubMed Central)
  2. Cryo-EM Structures of Centromeric Tri-nucleosomes Containing a Central CENP-A Nucleosome. Takizawa Y, Ho CH, Tachiwana H, Matsunami H, Kobayashi W, Suzuki M, Arimura Y, Hori T, Fukagawa T, Ohi MD, Wolf M, Kurumizaka H (2020) Structure 28(1): 44-53.e4
    › Primary publication · 31711756 (PubMed)
  3. Structure of the Cag type IV secretion system. Chung JM, Sheedlo MJ, Campbell AM, Sawhney N, Frick-Cheng AE, Lacy DB, Cover TL, Ohi MD (2019) Elife
    › Primary publication · 31210639 (PubMed) · PMC6620104 (PubMed Central)
  4. Allosteric Regulation of Oligomerization by a B Trafficking G-Protein Is Corrupted in Methylmalonic Aciduria. Ruetz M, Campanello GC, McDevitt L, Yokom AL, Yadav PK, Watkins D, Rosenblatt DS, Ohi MD, Southworth DR, Banerjee R (2019) Cell Chem Biol 26(7): 960-969.e4
    › Primary publication · 31056463 (PubMed) · PMC6642015 (PubMed Central)
  5. Cryo-EM Analysis Reveals Structural Basis of Helicobacter pylori VacA Toxin Oligomerization. Su M, Erwin AL, Campbell AM, Pyburn TM, Salay LE, Hanks JL, Lacy DB, Akey DL, Cover TL, Ohi MD (2019) J Mol Biol 431(10): 1956-1965
    › Primary publication · 30954575 (PubMed) · PMC6625667 (PubMed Central)
  6. Intracellular Degradation of Helicobacter pylori VacA Toxin as a Determinant of Gastric Epithelial Cell Viability. Foegeding NJ, Raghunathan K, Campbell AM, Kim SW, Lau KS, Kenworthy AK, Cover TL, Ohi MD (2019) Infect Immun 87(4)
    › Primary publication · 30692181 (PubMed) · PMC6434121 (PubMed Central)
  7. Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein. Gilchuk P, Kuzmina N, Ilinykh PA, Huang K, Gunn BM, Bryan A, Davidson E, Doranz BJ, Turner HL, Fusco ML, Bramble MS, Hoff NA, Binshtein E, Kose N, Flyak AI, Flinko R, Orlandi C, Carnahan R, Parrish EH, Sevy AM, Bombardi RG, Singh PK, Mukadi P, Muyembe-Tamfum JJ, Ohi MD, Saphire EO, Lewis GK, Alter G, Ward AB, Rimoin AW, Bukreyev A, Crowe JE (2018) Immunity 49(2): 363-374.e10
    › Primary publication · 30029854 (PubMed) · PMC6104738 (PubMed Central)
  8. Microtubule minus-end aster organization is driven by processive HSET-tubulin clusters. Norris SR, Jung S, Singh P, Strothman CE, Erwin AL, Ohi MD, Zanic M, Ohi R (2018) Nat Commun 9(1): 2659
    › Primary publication · 29985404 (PubMed) · PMC6037785 (PubMed Central)
  9. Determinants of Raft Partitioning of the Helicobacter pylori Pore-Forming Toxin VacA. Raghunathan K, Foegeding NJ, Campbell AM, Cover TL, Ohi MD, Kenworthy AK (2018) Infect Immun 86(5)
    › Primary publication · 29531133 (PubMed) · PMC5913846 (PubMed Central)
  10. Human antibody recognition of antigenic site IV on Pneumovirus fusion proteins. Mousa JJ, Binshtein E, Human S, Fong RH, Alvarado G, Doranz BJ, Moore ML, Ohi MD, Crowe JE (2018) PLoS Pathog 14(2): e1006837
    › Primary publication · 29470533 (PubMed) · PMC5823459 (PubMed Central)
  11. Structural basis of arrestin-3 activation and signaling. Chen Q, Perry NA, Vishnivetskiy SA, Berndt S, Gilbert NC, Zhuo Y, Singh PK, Tholen J, Ohi MD, Gurevich EV, Brautigam CA, Klug CS, Gurevich VV, Iverson TM (2017) Nat Commun 8(1): 1427
    › Primary publication · 29127291 (PubMed) · PMC5681653 (PubMed Central)
  12. Structural and functional analyses of the spliceosome requires a multi-disciplinary approach. Ohi MD (2017) Methods : 1-2
    › Primary publication · 28780959 (PubMed) · PMC5881893 (PubMed Central)
  13. Use of a neutralizing antibody helps identify structural features critical for binding of toxin TcdA to the host cell surface. Kroh HK, Chandrasekaran R, Rosenthal K, Woods R, Jin X, Ohi MD, Nyborg AC, Rainey GJ, Warrener P, Spiller BW, Lacy DB (2017) J Biol Chem 292(35): 14401-14412
    › Primary publication · 28705932 (PubMed) · PMC5582835 (PubMed Central)
  14. Peripheral myelin protein 22 alters membrane architecture. Mittendorf KF, Marinko JT, Hampton CM, Ke Z, Hadziselimovic A, Schlebach JP, Law CL, Li J, Wright ER, Sanders CR, Ohi MD (2017) Sci Adv 3(7): e1700220
    › Primary publication · 28695207 (PubMed) · PMC5498104 (PubMed Central)
  15. Epitopes and Mechanism of Action of the Clostridium difficile Toxin A-Neutralizing Antibody Actoxumab. Hernandez LD, Kroh HK, Hsieh E, Yang X, Beaumont M, Sheth PR, DiNunzio E, Rutherford SA, Ohi MD, Ermakov G, Xiao L, Secore S, Karczewski J, Racine F, Mayhood T, Fischer P, Sher X, Gupta P, Lacy DB, Therien AG (2017) J Mol Biol 429(7): 1030-1044
    › Primary publication · 28232034 (PubMed) · PMC7373199 (PubMed Central)
  16. Structural basis for nonneutralizing antibody competition at antigenic site II of the respiratory syncytial virus fusion protein. Mousa JJ, Sauer MF, Sevy AM, Finn JA, Bates JT, Alvarado G, King HG, Loerinc LB, Fong RH, Doranz BJ, Correia BE, Kalyuzhniy O, Wen X, Jardetzky TS, Schief WR, Ohi MD, Meiler J, Crowe JE (2016) Proc Natl Acad Sci U S A 113(44): E6849-E6858
    › Primary publication · 27791117 (PubMed) · PMC5098655 (PubMed Central)
  17. While the revolution will not be crystallized, biochemistry reigns supreme. Takizawa Y, Binshtein E, Erwin AL, Pyburn TM, Mittendorf KF, Ohi MD (2017) Protein Sci 26(1): 69-81
    › Primary publication · 27673321 (PubMed) · PMC5192976 (PubMed Central)
  18. A Nonoligomerizing Mutant Form of Helicobacter pylori VacA Allows Structural Analysis of the p33 Domain. González-Rivera C, Campbell AM, Rutherford SA, Pyburn TM, Foegeding NJ, Barke TL, Spiller BW, McClain MS, Ohi MD, Lacy DB, Cover TL (2016) Infect Immun 84(9): 2662-70
    › Primary publication · 27382020 (PubMed) · PMC4995914 (PubMed Central)
  19. Structural organization of membrane-inserted hexamers formed by Helicobacter pylori VacA toxin. Pyburn TM, Foegeding NJ, González-Rivera C, McDonald NA, Gould KL, Cover TL, Ohi MD (2016) Mol Microbiol 102(1): 22-36
    › Primary publication · 27309820 (PubMed) · PMC5035229 (PubMed Central)
  20. An Overview of Helicobacter pylori VacA Toxin Biology. Foegeding NJ, Caston RR, McClain MS, Ohi MD, Cover TL (2016) Toxins (Basel) 8(6)
    › Primary publication · 27271669 (PubMed) · PMC4926140 (PubMed Central)
  21. The Tubulation Activity of a Fission Yeast F-BAR Protein Is Dispensable for Its Function in Cytokinesis. McDonald NA, Takizawa Y, Feoktistova A, Xu P, Ohi MD, Vander Kooi CW, Gould KL (2016) Cell Rep 14(3): 534-546
    › Primary publication · 26776521 (PubMed) · PMC4731314 (PubMed Central)
  22. Molecular and Structural Analysis of the Helicobacter pylori cag Type IV Secretion System Core Complex. Frick-Cheng AE, Pyburn TM, Voss BJ, McDonald WH, Ohi MD, Cover TL (2016) mBio 7(1): e02001-15
    › Primary publication · 26758182 (PubMed) · PMC4725015 (PubMed Central)
  23. Oligomerization but Not Membrane Bending Underlies the Function of Certain F-BAR Proteins in Cell Motility and Cytokinesis. McDonald NA, Vander Kooi CW, Ohi MD, Gould KL (2015) Dev Cell 35(6): 725-36
    › Primary publication · 26702831 (PubMed) · PMC4691284 (PubMed Central)
  24. Biased Brownian motion as a mechanism to facilitate nanometer-scale exploration of the microtubule plus end by a kinesin-8. Shin Y, Du Y, Collier SE, Ohi MD, Lang MJ, Ohi R (2015) Proc Natl Acad Sci U S A 112(29): E3826-35
    › Primary publication · 26150501 (PubMed) · PMC4517267 (PubMed Central)
  25. Cryo-electron microscopy and the amazing race to atomic resolution. Binshtein E, Ohi MD (2015) Biochemistry 54(20): 3133-41
    › Primary publication · 25955078 (PubMed)
  26. Glycolytic enzymes localize to ribonucleoprotein granules in Drosophila germ cells, bind Tudor and protect from transposable elements. Gao M, Thomson TC, Creed TM, Tu S, Loganathan SN, Jackson CA, McCluskey P, Lin Y, Collier SE, Weng Z, Lasko P, Ohi MD, Arkov AL (2015) EMBO Rep 16(3): 379-86
    › Primary publication · 25600116 (PubMed) · PMC4364877 (PubMed Central)
  27. Kinesin-12 Kif15 targets kinetochore fibers through an intrinsic two-step mechanism. Sturgill EG, Das DK, Takizawa Y, Shin Y, Collier SE, Ohi MD, Hwang W, Lang MJ, Ohi R (2014) Curr Biol 24(19): 2307-13
    › Primary publication · 25264249 (PubMed) · PMC4207087 (PubMed Central)
  28. Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5. Collier SE, Voehler M, Peng D, Ohi R, Gould KL, Reiter NJ, Ohi MD (2014) Biochemistry 53(41): 6439-51
    › Primary publication · 25263959 (PubMed) · PMC4204884 (PubMed Central)
  29. Gle1 functions during mRNA export in an oligomeric complex that is altered in human disease. Folkmann AW, Collier SE, Zhan X, Aditi , Ohi MD, Wente SR (2013) Cell 155(3): 582-93
    › Primary publication · 24243016 (PubMed) · PMC3855398 (PubMed Central)
  30. Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10. Livesay SB, Collier SE, Bitton DA, Bähler J, Ohi MD (2013) Eukaryot Cell 12(11): 1472-89
    › Primary publication · 24014766 (PubMed) · PMC3837936 (PubMed Central)
  31. Molecular assembly of botulinum neurotoxin progenitor complexes. Benefield DA, Dessain SK, Shine N, Ohi MD, Lacy DB (2013) Proc Natl Acad Sci U S A 110(14): 5630-5
    › Primary publication · 23509303 (PubMed) · PMC3619295 (PubMed Central)
  32. Nature of KaiB-KaiC binding in the cyanobacterial circadian oscillator. Pattanayek R, Yadagiri KK, Ohi MD, Egli M (2013) Cell Cycle 12(5): 810-7
    › Primary publication · 23388462 (PubMed) · PMC3610728 (PubMed Central)
  33. Structural analysis of the oligomeric states of Helicobacter pylori VacA toxin. Chambers MG, Pyburn TM, González-Rivera C, Collier SE, Eli I, Yip CK, Takizawa Y, Lacy DB, Cover TL, Ohi MD (2013) J Mol Biol 425(3): 524-35
    › Primary publication · 23178866 (PubMed) · PMC3612943 (PubMed Central)
  34. The human metapneumovirus fusion protein mediates entry via an interaction with RGD-binding integrins. Cox RG, Livesay SB, Johnson M, Ohi MD, Williams JV (2012) J Virol 86(22): 12148-60
    › Primary publication · 22933271 (PubMed) · PMC3486500 (PubMed Central)
  35. Tailoring of membrane proteins by alternative splicing of pre-mRNA. Mittendorf KF, Deatherage CL, Ohi MD, Sanders CR (2012) Biochemistry 51(28): 5541-56
    › Primary publication · 22708632 (PubMed) · PMC3448030 (PubMed Central)
  36. Fission yeast Dma1 requires RING domain dimerization for its ubiquitin ligase activity and mitotic checkpoint function. Johnson AE, Collier SE, Ohi MD, Gould KL (2012) J Biol Chem 287(31): 25741-8
    › Primary publication · 22669973 (PubMed) · PMC3406662 (PubMed Central)
  37. Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. Chen JS, Lu LX, Ohi MD, Creamer KM, English C, Partridge JF, Ohi R, Gould KL (2011) J Cell Biol 195(4): 583-93
    › Primary publication · 22065639 (PubMed) · PMC3257533 (PubMed Central)
  38. The Chlamydia effector chlamydial outer protein N (CopN) sequesters tubulin and prevents microtubule assembly. Archuleta TL, Du Y, English CA, Lory S, Lesser C, Ohi MD, Ohi R, Spiller BW (2011) J Biol Chem 286(39): 33992-8
    › Primary publication · 21841198 (PubMed) · PMC3190796 (PubMed Central)
  39. Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19. Ren L, McLean JR, Hazbun TR, Fields S, Vander Kooi C, Ohi MD, Gould KL (2011) PLoS One 6(2): e16719
    › Primary publication · 21386897 (PubMed) · PMC3046128 (PubMed Central)
  40. State of the APC/C: organization, function, and structure. McLean JR, Chaix D, Ohi MD, Gould KL (2011) Crit Rev Biochem Mol Biol 46(2): 118-36
    › Primary publication · 21261459 (PubMed) · PMC4856037 (PubMed Central)
  41. Structural organization of the functional domains of Clostridium difficile toxins A and B. Pruitt RN, Chambers MG, Ng KK, Ohi MD, Lacy DB (2010) Proc Natl Acad Sci U S A 107(30): 13467-72
    › Primary publication · 20624955 (PubMed) · PMC2922184 (PubMed Central)
  42. Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site. Roberts-Galbraith RH, Ohi MD, Ballif BA, Chen JS, McLeod I, McDonald WH, Gygi SP, Yates JR, Gould KL (2010) Mol Cell 39(1): 86-99
    › Primary publication · 20603077 (PubMed) · PMC2916701 (PubMed Central)
  43. Reconstitution of Helicobacter pylori VacA toxin from purified components. González-Rivera C, Gangwer KA, McClain MS, Eli IM, Chambers MG, Ohi MD, Lacy DB, Cover TL (2010) Biochemistry 49(27): 5743-52
    › Primary publication · 20527875 (PubMed) · PMC2910095 (PubMed Central)
  44. The Prp19 WD40 domain contains a conserved protein interaction region essential for its function. Vander Kooi CW, Ren L, Xu P, Ohi MD, Gould KL, Chazin WJ (2010) Structure 18(5): 584-93
    › Primary publication · 20462492 (PubMed) · PMC2871033 (PubMed Central)
  45. Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1. Ohi MD, Feoktistova A, Ren L, Yip C, Cheng Y, Chen JS, Yoon HJ, Wall JS, Huang Z, Penczek PA, Gould KL, Walz T (2007) Mol Cell 28(5): 871-85
    › Primary publication · 18082611 (PubMed) · PMC2197158 (PubMed Central)
  46. Structural characterization of the fission yeast U5.U2/U6 spliceosome complex. Ohi MD, Ren L, Wall JS, Gould KL, Walz T (2007) Proc Natl Acad Sci U S A 104(9): 3195-200
    › Primary publication · 17360628 (PubMed) · PMC1805518 (PubMed Central)
  47. Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity. Huang DT, Hunt HW, Zhuang M, Ohi MD, Holton JM, Schulman BA (2007) Nature 445(7126): 394-8
    › Primary publication · 17220875 (PubMed) · PMC2821831 (PubMed Central)
  48. The Prp19 U-box crystal structure suggests a common dimeric architecture for a class of oligomeric E3 ubiquitin ligases. Vander Kooi CW, Ohi MD, Rosenberg JA, Oldham ML, Newcomer ME, Gould KL, Chazin WJ (2006) Biochemistry 45(1): 121-30
    › Primary publication · 16388587 (PubMed) · PMC2570371 (PubMed Central)
  49. Ubiquitin-like protein Hub1 is required for pre-mRNA splicing and localization of an essential splicing factor in fission yeast. Wilkinson CR, Dittmar GA, Ohi MD, Uetz P, Jones N, Finley D (2004) Curr Biol 14(24): 2283-8
    › Primary publication · 15620657 (PubMed)
  50. Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Ohi MD, Vander Kooi CW, Rosenberg JA, Ren L, Hirsch JP, Chazin WJ, Walz T, Gould KL (2005) Mol Cell Biol 25(1): 451-60
    › Primary publication · 15601865 (PubMed) · PMC538785 (PubMed Central)
  51. Structural insights into the U-box, a domain associated with multi-ubiquitination. Ohi MD, Vander Kooi CW, Rosenberg JA, Chazin WJ, Gould KL (2003) Nat Struct Biol 10(4): 250-5
    › Primary publication · 12627222 (PubMed) · PMC5881891 (PubMed Central)
  52. Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. Ohi MD, Gould KL (2002) RNA 8(6): 798-815
    › Primary publication · 12088152 (PubMed) · PMC1370298 (PubMed Central)
  53. Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Ohi MD, Link AJ, Ren L, Jennings JL, McDonald WH, Gould KL (2002) Mol Cell Biol 22(7): 2011-24
    › Primary publication · 11884590 (PubMed) · PMC133674 (PubMed Central)