Jeffrey Spraggins
Last active: 3/18/2020

Profile

My research focuses on the development of next-generation molecular imaging technologies and advanced applications for imaging mass spectrometry (IMS). I have been performing research in the field of imaging mass spectrometry for 10 years and have more than 15 years of experience in biological mass spectrometry. I have also been Team Leader for all instrumentation development efforts in this NIH funded National Research Resource for Imaging Mass Spectrometry. My training and expertise is in FT-ICR MS instrumentation and much of my work has centered on advancing the capabilities and applications of high performance platforms for molecular imaging. Traditionally thought of as only applicable for small molecule and lipid MALDI experiments, recently our team has developed instrumental modifications and methods that enable FT-ICR imaging of intact proteins (Analytical Chemistry, 2018 and Proteomics, 2016). These results have dramatically enhanced our ability to identify proteins in imaging experiments by providing the spectral performance necessary to resolve complex protein mixtures (including PTMs) and by linking IMS to spatially targeted LC-MS/MS data by mass accuracy (Analytical Chemistry, 2019). I have also been part of a team focused on multimodal image fusion (Nature Methods, 2015).  This work aims to create new imaging modalities that synergistically combine data from different imaging experiments (e.g. microscopy and IMS). More recently, I have lead a project in collaboration with Bruker Daltonics to develop a trapped ion mobility spectrometry MALDI platform for high spatial resolution and specificity imaging (Analytical Chemistry, 2019). Our team has begun to apply these diverse advanced imaging technologies for the construction and analysis of 3-D multimodal molecular atlases. As part of the Human Biomolecular Atlas Program (HuBMAP, NIH), I am Director of Research for the Vanderbilt University Biomolecular Mutlimodal Imaging Center (BIOMIC) where we are bringing together 3-D imaging mass spectrometry with a variety of in vivo and ex vivo imaging technologies to create new modalities of molecularly-informed medical imaging. This unique combination of analytical technologies is dramatically expanding our ability to elucidate the molecular drivers of biology and disease.

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Integrated molecular imaging technologies for investigation of metals in biological systems: A brief review. Perry WJ, Weiss A, Van de Plas R, Spraggins JM, Caprioli RM, Skaar EP (2020) Curr Opin Chem Biol : 127-135
    › Primary publication · 32087551 (PubMed) · PMC7237308 (PubMed Central)
  2. Uncovering matrix effects on lipid analyses in MALDI imaging mass spectrometry experiments. Perry WJ, Patterson NH, Prentice BM, Neumann EK, Caprioli RM, Spraggins JM (2020) J Mass Spectrom 55(4): e4491
    › Primary publication · 31860760 (PubMed) · PMC7383219 (PubMed Central)
  3. exhibits heterogeneous siderophore production within the vertebrate host. Perry WJ, Spraggins JM, Sheldon JR, Grunenwald CM, Heinrichs DE, Cassat JE, Skaar EP, Caprioli RM (2019) Proc Natl Acad Sci U S A 116(44): 21980-21982
    › Primary publication · 31611408 (PubMed) · PMC6825271 (PubMed Central)
  4. The human body at cellular resolution: the NIH Human Biomolecular Atlas Program. HuBMAP Consortium (2019) Nature 574(7777): 187-192
    › Primary publication · 31597973 (PubMed) · PMC6800388 (PubMed Central)
  5. High-Performance Molecular Imaging with MALDI Trapped Ion-Mobility Time-of-Flight (timsTOF) Mass Spectrometry. Spraggins JM, Djambazova KV, Rivera ES, Migas LG, Neumann EK, Fuetterer A, Suetering J, Goedecke N, Ly A, Van de Plas R, Caprioli RM (2019) Anal Chem 91(22): 14552-14560
    › Primary publication · 31593446 (PubMed) · PMC7382025 (PubMed Central)
  6. Two Specific Sulfatide Species Are Dysregulated during Renal Development in a Mouse Model of Alport Syndrome. Gessel MM, Spraggins JM, Voziyan PA, Abrahamson DR, Caprioli RM, Hudson BG (2019) Lipids 54(6-7): 411-418
    › Primary publication · 31197846 (PubMed) · PMC6655412 (PubMed Central)
  7. MicroLESA: Integrating Autofluorescence Microscopy, In Situ Micro-Digestions, and Liquid Extraction Surface Analysis for High Spatial Resolution Targeted Proteomic Studies. Ryan DJ, Patterson NH, Putnam NE, Wilde AD, Weiss A, Perry WJ, Cassat JE, Skaar EP, Caprioli RM, Spraggins JM (2019) Anal Chem 91(12): 7578-7585
    › Primary publication · 31149808 (PubMed) · PMC6652190 (PubMed Central)
  8. Imaging mass spectrometry enables molecular profiling of mouse and human pancreatic tissue. Prentice BM, Hart NJ, Phillips N, Haliyur R, Judd A, Armandala R, Spraggins JM, Lowe CL, Boyd KL, Stein RW, Wright CV, Norris JL, Powers AC, Brissova M, Caprioli RM (2019) Diabetologia 62(6): 1036-1047
    › Primary publication · 30955045 (PubMed) · PMC6553460 (PubMed Central)
  9. Protein identification strategies in MALDI imaging mass spectrometry: a brief review. Ryan DJ, Spraggins JM, Caprioli RM (2019) Curr Opin Chem Biol : 64-72
    › Primary publication · 30476689 (PubMed) · PMC6382520 (PubMed Central)
  10. Viewing the Future of IR through Molecular Histology: An Overview of Imaging Mass Spectrometry. Cressman ENK, Spraggins JM (2018) J Vasc Interv Radiol 29(11): 1543-1546.e1
    › Primary publication · 30274858 (PubMed) · PMC6800995 (PubMed Central)
  11. Response of Secondary Metabolism of Hypogean Actinobacterial Genera to Chemical and Biological Stimuli. Covington BC, Spraggins JM, Ynigez-Gutierrez AE, Hylton ZB, Bachmann BO (2018) Appl Environ Microbiol 84(19)
    › Primary publication · 30030223 (PubMed) · PMC6146984 (PubMed Central)
  12. Regional differences in brain glucose metabolism determined by imaging mass spectrometry. Kleinridders A, Ferris HA, Reyzer ML, Rath M, Soto M, Manier ML, Spraggins J, Yang Z, Stanton RC, Caprioli RM, Kahn CR (2018) Mol Metab : 113-121
    › Primary publication · 29681509 (PubMed) · PMC6001904 (PubMed Central)
  13. Integrated molecular imaging reveals tissue heterogeneity driving host-pathogen interactions. Cassat JE, Moore JL, Wilson KJ, Stark Z, Prentice BM, Van de Plas R, Perry WJ, Zhang Y, Virostko J, Colvin DC, Rose KL, Judd AM, Reyzer ML, Spraggins JM, Grunenwald CM, Gore JC, Caprioli RM, Skaar EP (2018) Sci Transl Med 10(432)
    › Primary publication · 29540616 (PubMed) · PMC6005374 (PubMed Central)
  14. Enhanced Ion Transmission Efficiency up to m/ z 24 000 for MALDI Protein Imaging Mass Spectrometry. Prentice BM, Ryan DJ, Van de Plas R, Caprioli RM, Spraggins JM (2018) Anal Chem 90(8): 5090-5099
    › Primary publication · 29444410 (PubMed) · PMC6905630 (PubMed Central)
  15. Bis(monoacylglycero)phosphate lipids in the retinal pigment epithelium implicate lysosomal/endosomal dysfunction in a model of Stargardt disease and human retinas. Anderson DMG, Ablonczy Z, Koutalos Y, Hanneken AM, Spraggins JM, Calcutt MW, Crouch RK, Caprioli RM, Schey KL (2017) Sci Rep 7(1): 17352
    › Primary publication · 29229934 (PubMed) · PMC5725462 (PubMed Central)
  16. Protein identification in imaging mass spectrometry through spatially targeted liquid micro-extractions. Ryan DJ, Nei D, Prentice BM, Rose KL, Caprioli RM, Spraggins JM (2018) Rapid Commun Mass Spectrom 32(5): 442-450
    › Primary publication · 29226434 (PubMed) · PMC5812809 (PubMed Central)
  17. Connecting imaging mass spectrometry and magnetic resonance imaging-based anatomical atlases for automated anatomical interpretation and differential analysis. Verbeeck N, Spraggins JM, Murphy MJM, Wang HD, Deutch AY, Caprioli RM, Van de Plas R (2017) Biochim Biophys Acta Proteins Proteom 1865(7): 967-977
    › Primary publication · 28254588 (PubMed)
  18. Integrated, High-Throughput, Multiomics Platform Enables Data-Driven Construction of Cellular Responses and Reveals Global Drug Mechanisms of Action. Norris JL, Farrow MA, Gutierrez DB, Palmer LD, Muszynski N, Sherrod SD, Pino JC, Allen JL, Spraggins JM, Lubbock AL, Jordan A, Burns W, Poland JC, Romer C, Manier ML, Nei YW, Prentice BM, Rose KL, Hill S, Van de Plas R, Tsui T, Braman NM, Keller MR, Rutherford SA, Lobdell N, Lopez CF, Lacy DB, McLean JA, Wikswo JP, Skaar EP, Caprioli RM (2017) J Proteome Res 16(3): 1364-1375
    › Primary publication · 28088864 (PubMed)
  19. Next-generation technologies for spatial proteomics: Integrating ultra-high speed MALDI-TOF and high mass resolution MALDI FTICR imaging mass spectrometry for protein analysis. Spraggins JM, Rizzo DG, Moore JL, Noto MJ, Skaar EP, Caprioli RM (2016) Proteomics 16(11-12): 1678-89
    › Primary publication · 27060368 (PubMed) · PMC5117945 (PubMed Central)
  20. Phospholipid profiling identifies acyl chain elongation as a ubiquitous trait and potential target for the treatment of lung squamous cell carcinoma. Marien E, Meister M, Muley T, Gomez Del Pulgar T, Derua R, Spraggins JM, Van de Plas R, Vanderhoydonc F, Machiels J, Binda MM, Dehairs J, Willette-Brown J, Hu Y, Dienemann H, Thomas M, Schnabel PA, Caprioli RM, Lacal JC, Waelkens E, Swinnen JV (2016) Oncotarget 7(11): 12582-97
    › Primary publication · 26862848 (PubMed) · PMC4914306 (PubMed Central)
  21. MALDI Imaging Mass Spectrometry Spatially Maps Age-Related Deamidation and Truncation of Human Lens Aquaporin-0. Wenke JL, Rose KL, Spraggins JM, Schey KL (2015) Invest Ophthalmol Vis Sci 56(12): 7398-405
    › Primary publication · 26574799 (PubMed) · PMC4675204 (PubMed Central)
  22. Decellularization of intact tissue enables MALDI imaging mass spectrometry analysis of the extracellular matrix. Gessel M, Spraggins JM, Voziyan P, Hudson BG, Caprioli RM (2015) J Mass Spectrom 50(11): 1288-93
    › Primary publication · 26505774 (PubMed) · PMC5320948 (PubMed Central)
  23. Determination of N-retinylidene-N-retinylethanolamine (A2E) levels in central and peripheral areas of human retinal pigment epithelium. Adler L, Boyer NP, Anderson DM, Spraggins JM, Schey KL, Hanneken A, Ablonczy Z, Crouch RK, Koutalos Y (2015) Photochem Photobiol Sci 14(11): 1983-90
    › Primary publication · 26323192 (PubMed) · PMC4626405 (PubMed Central)
  24. MALDI FTICR IMS of Intact Proteins: Using Mass Accuracy to Link Protein Images with Proteomics Data. Spraggins JM, Rizzo DG, Moore JL, Rose KL, Hammer ND, Skaar EP, Caprioli RM (2015) J Am Soc Mass Spectrom 26(6): 974-85
    › Primary publication · 25904064 (PubMed) · PMC4442642 (PubMed Central)
  25. High spatial resolution imaging mass spectrometry of human optic nerve lipids and proteins. Anderson DM, Spraggins JM, Rose KL, Schey KL (2015) J Am Soc Mass Spectrom 26(6): 940-7
    › Primary publication · 25893273 (PubMed) · PMC5650057 (PubMed Central)
  26. Non-small cell lung cancer is characterized by dramatic changes in phospholipid profiles. Marien E, Meister M, Muley T, Fieuws S, Bordel S, Derua R, Spraggins J, Van de Plas R, Dehairs J, Wouters J, Bagadi M, Dienemann H, Thomas M, Schnabel PA, Caprioli RM, Waelkens E, Swinnen JV (2015) Int J Cancer 137(7): 1539-48
    › Primary publication · 25784292 (PubMed) · PMC4503522 (PubMed Central)
  27. Image fusion of mass spectrometry and microscopy: a multimodality paradigm for molecular tissue mapping. Van de Plas R, Yang J, Spraggins J, Caprioli RM (2015) Nat Methods 12(4): 366-72
    › Primary publication · 25707028 (PubMed) · PMC4382398 (PubMed Central)
  28. A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry. Manier ML, Spraggins JM, Reyzer ML, Norris JL, Caprioli RM (2014) J Mass Spectrom 49(8): 665-73
    › Primary publication · 25044893 (PubMed) · PMC4126081 (PubMed Central)
  29. Diabetic nephropathy induces alterations in the glomerular and tubule lipid profiles. Grove KJ, Voziyan PA, Spraggins JM, Wang S, Paueksakon P, Harris RC, Hudson BG, Caprioli RM (2014) J Lipid Res 55(7): 1375-85
    › Primary publication · 24864273 (PubMed) · PMC4076088 (PubMed Central)
  30. High resolution MALDI imaging mass spectrometry of retinal tissue lipids. Anderson DM, Ablonczy Z, Koutalos Y, Spraggins J, Crouch RK, Caprioli RM, Schey KL (2014) J Am Soc Mass Spectrom 25(8): 1394-403
    › Primary publication · 24819461 (PubMed) · PMC4180438 (PubMed Central)
  31. The utilization of fluorescence to identify the components of lipofuscin by imaging mass spectrometry. Ablonczy Z, Smith N, Anderson DM, Grey AC, Spraggins J, Koutalos Y, Schey KL, Crouch RK (2014) Proteomics 14(7-8): 936-44
    › Primary publication · 24453194 (PubMed) · PMC4017854 (PubMed Central)
  32. High-resolution matrix-assisted laser desorption ionization-imaging mass spectrometry of lipids in rodent optic nerve tissue. Anderson DM, Mills D, Spraggins J, Lambert WS, Calkins DJ, Schey KL (2013) Mol Vis : 581-92
    › Primary publication · 23559852 (PubMed) · PMC3611942 (PubMed Central)
  33. Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies. Thiery G, Mernaugh RL, Yan H, Spraggins JM, Yang J, Parl FF, Caprioli RM (2012) J Am Soc Mass Spectrom 23(10): 1689-96
    › Primary publication · 22869296 (PubMed) · PMC3525520 (PubMed Central)
  34. Enhanced sensitivity for high spatial resolution lipid analysis by negative ion mode matrix assisted laser desorption ionization imaging mass spectrometry. Angel PM, Spraggins JM, Baldwin HS, Caprioli R (2012) Anal Chem 84(3): 1557-64
    › Primary publication · 22243218 (PubMed) · PMC3277660 (PubMed Central)
  35. High-speed MALDI-TOF imaging mass spectrometry: rapid ion image acquisition and considerations for next generation instrumentation. Spraggins JM, Caprioli RM (2011) J Am Soc Mass Spectrom 22(6): 1022-31
    › Primary publication · 21953043 (PubMed) · PMC3514015 (PubMed Central)
  36. MALDI imaging of lipid biochemistry in tissues by mass spectrometry. Berry KA, Hankin JA, Barkley RM, Spraggins JM, Caprioli RM, Murphy RC (2011) Chem Rev 111(10): 6491-512
    › Primary publication · 21942646 (PubMed) · PMC3199966 (PubMed Central)
  37. Fragmentation mechanisms of oxidized peptides elucidated by SID, RRKM modeling, and molecular dynamics. Spraggins JM, Lloyd JA, Johnston MV, Laskin J, Ridge DP (2009) J Am Soc Mass Spectrom 20(9): 1579-92
    › Primary publication · 19560936 (PubMed)
  38. Peptide ozonolysis: product structures and relative reactivities for oxidation of tyrosine and histidine residues. Lloyd JA, Spraggins JM, Johnston MV, Laskin J (2006) J Am Soc Mass Spectrom 17(9): 1289-98
    › Primary publication · 16820303 (PubMed)