Conrad Wagner
Last active: 1/20/2015

Profile

Research Description: Our laboratory has been involved with various aspects of one-carbon metabolism for many years. These include the roles of the coenzyme forms of the vitamin, folic acid, and the enzymes that carry out these functions. Our approach has been to investigate every available aspect of Biochemistry to accomplish this including enzymology, mass spectrometry, crystallography, molecular biology and nutritional manipulation. Our latest work has focused on developing a mouse knock-out of the enzyme GNMT which is responsible for the regulation of levels of S-adenosymethionine (SAM). This GNMT knockout mouse spontaneously develops fatty liver and hepatocellular carcinoma that is a result of methylation of tumor suppressors in the promoter region of the gene. SAM, a metabolic product of methionine metabolism, is the major donor of methyl groups in the body. This line of investigation has led us to the discovery of two new metabolic diseases. We have recently shown that the histone demethylase, LSD1, is a folate binding protein in which the folate probably serves to scavenge the formaldehyde that is a product of the demethylase reaction. This finding suggests that the levels of folate in the diet can play a role in gene expression. More recently we have shown that products of methionine breakdown in serum may serve as markers for the risk of developing metastasis of prostate cancer.

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. TRAIL-producing NK cells contribute to liver injury and related fibrogenesis in the context of GNMT deficiency. Fernández-Álvarez S, Gutiérrez-de Juan V, Zubiete-Franco I, Barbier-Torres L, Lahoz A, Parés A, Luka Z, Wagner C, Lu SC, Mato JM, Martínez-Chantar ML, Beraza N (2015) Lab Invest 95(2): 223-36
    › Primary publication · 25531568 (PubMed) · PMC4310762 (PubMed Central)
  2. S-Adenosylmethionine increases circulating very-low density lipoprotein clearance in non-alcoholic fatty liver disease. Martínez-Uña M, Varela-Rey M, Mestre D, Fernández-Ares L, Fresnedo O, Fernandez-Ramos D, Gutiérrez-de Juan V, Martin-Guerrero I, García-Orad A, Luka Z, Wagner C, Lu SC, García-Monzón C, Finnell RH, Aurrekoetxea I, Buqué X, Martínez-Chantar ML, Mato JM, Aspichueta P (2015) J Hepatol 62(3): 673-81
    › Primary publication · 25457203 (PubMed) · PMC4336596 (PubMed Central)
  3. Folate in demethylation: the crystal structure of the rat dimethylglycine dehydrogenase complexed with tetrahydrofolate. Luka Z, Pakhomova S, Loukachevitch LV, Newcomer ME, Wagner C (2014) Biochem Biophys Res Commun 449(4): 392-8
    › Primary publication · 24858690 (PubMed) · PMC4113215 (PubMed Central)
  4. Crystal structure of the histone lysine specific demethylase LSD1 complexed with tetrahydrofolate. Luka Z, Pakhomova S, Loukachevitch LV, Calcutt MW, Newcomer ME, Wagner C (2014) Protein Sci 23(7): 993-8
    › Primary publication · 24715612 (PubMed) · PMC4088983 (PubMed Central)
  5. Plasma folate concentrations and colorectal cancer risk: a case-control study nested within the Shanghai Men's Health Study. Takata Y, Shrubsole MJ, Li H, Cai Q, Gao J, Wagner C, Wu J, Zheng W, Xiang YB, Shu XO (2014) Int J Cancer 135(9): 2191-8
    › Primary publication · 24692023 (PubMed) · PMC4134766 (PubMed Central)
  6. Glycine N-methyltransferase expression in the hippocampus and its role in neurogenesis and cognitive performance. Carrasco M, Rabaneda LG, Murillo-Carretero M, Ortega-Martínez S, Martínez-Chantar ML, Woodhoo A, Luka Z, Wagner C, Lu SC, Mato JM, Micó JA, Castro C (2014) Hippocampus 24(7): 840-52
    › Primary publication · 24687756 (PubMed) · PMC4065208 (PubMed Central)
  7. S-adenosylmethionine levels regulate the schwann cell DNA methylome. Varela-Rey M, Iruarrizaga-Lejarreta M, Lozano JJ, Aransay AM, Fernandez AF, Lavin JL, Mósen-Ansorena D, Berdasco M, Turmaine M, Luka Z, Wagner C, Lu SC, Esteller M, Mirsky R, Jessen KR, Fraga MF, Martínez-Chantar ML, Mato JM, Woodhoo A (2014) Neuron 81(5): 1024-1039
    › Primary publication · 24607226 (PubMed) · PMC3960855 (PubMed Central)
  8. Metabolic profiling of total homocysteine and related compounds in hyperhomocysteinemia: utility and limitations in diagnosing the cause of puzzling thrombophilia in a family. Stabler SP, Korson M, Jethva R, Allen RH, Kraus JP, Spector EB, Wagner C, Mudd SH (2013) JIMD Rep : 149-63
    › Primary publication · 23733603 (PubMed) · PMC3755560 (PubMed Central)
  9. Excess S-adenosylmethionine reroutes phosphatidylethanolamine towards phosphatidylcholine and triglyceride synthesis. Martínez-Uña M, Varela-Rey M, Cano A, Fernández-Ares L, Beraza N, Aurrekoetxea I, Martínez-Arranz I, García-Rodríguez JL, Buqué X, Mestre D, Luka Z, Wagner C, Alonso C, Finnell RH, Lu SC, Martínez-Chantar ML, Aspichueta P, Mato JM (2013) Hepatology 58(4): 1296-305
    › Primary publication · 23505042 (PubMed) · PMC3720726 (PubMed Central)
  10. Hepatoma cells from mice deficient in glycine N-methyltransferase have increased RAS signaling and activation of liver kinase B1. Martínez-López N, García-Rodríguez JL, Varela-Rey M, Gutiérrez V, Fernández-Ramos D, Beraza N, Aransay AM, Schlangen K, Lozano JJ, Aspichueta P, Luka Z, Wagner C, Evert M, Calvisi DF, Lu SC, Mato JM, Martínez-Chantar ML (2012) Gastroenterology 143(3): 787-798.e13
    › Primary publication · 22687285 (PubMed) · PMC3429651 (PubMed Central)
  11. Inhibition of natural killer cells protects the liver against acute injury in the absence of glycine N-methyltransferase. Gomez-Santos L, Luka Z, Wagner C, Fernandez-Alvarez S, Lu SC, Mato JM, Martinez-Chantar ML, Beraza N (2012) Hepatology 56(2): 747-59
    › Primary publication · 22392635 (PubMed) · PMC3378767 (PubMed Central)
  12. Two patients with hepatic mtDNA depletion syndromes and marked elevations of S-adenosylmethionine and methionine. Mudd SH, Wagner C, Luka Z, Stabler SP, Allen RH, Schroer R, Wood T, Wang J, Wong LJ (2012) Mol Genet Metab 105(2): 228-36
    › Primary publication · 22137549 (PubMed) · PMC3264801 (PubMed Central)
  13. Differences in folate-protein interactions result in differing inhibition of native rat liver and recombinant glycine N-methyltransferase by 5-methyltetrahydrofolate. Luka Z, Pakhomova S, Loukachevitch LV, Newcomer ME, Wagner C (2012) Biochim Biophys Acta 1824(2): 286-91
    › Primary publication · 22037183 (PubMed) · PMC3253920 (PubMed Central)
  14. Serum methionine metabolites are risk factors for metastatic prostate cancer progression. Stabler S, Koyama T, Zhao Z, Martinez-Ferrer M, Allen RH, Luka Z, Loukachevitch LV, Clark PE, Wagner C, Bhowmick NA (2011) PLoS One 6(8): e22486
    › Primary publication · 21853037 (PubMed) · PMC3154200 (PubMed Central)
  15. High-frequency ultrasound imaging for longitudinal evaluation of non-alcoholic fatty liver disease progression in mice. Fernández-Domínguez I, Echevarria-Uraga JJ, Gómez N, Luka Z, Wagner C, Lu SC, Mato JM, Martínez-Chantar ML, Rodríguez-Cuesta J (2011) Ultrasound Med Biol 37(7): 1161-9
    › Primary publication · 21645964 (PubMed) · PMC3118979 (PubMed Central)
  16. Histone demethylase LSD1 is a folate-binding protein. Luka Z, Moss F, Loukachevitch LV, Bornhop DJ, Wagner C (2011) Biochemistry 50(21): 4750-6
    › Primary publication · 21510664 (PubMed) · PMC3111596 (PubMed Central)
  17. Sarcosine, folate metabolism and prostate cancer--is there a link? Wagner C, Luka Z (2011) J Urol 185(2): 385-6
    › Primary publication · 21168165 (PubMed)
  18. Some more comments on 'folate deficiency in chronic pancreatitis'. Wagner C (2010) JOP 11(6): 646-7; author reply 651-3
    › Primary publication · 21068506 (PubMed)
  19. S-adenosylhomocysteine hydrolase deficiency: two siblings with fetal hydrops and fatal outcomes. Grubbs R, Vugrek O, Deisch J, Wagner C, Stabler S, Allen R, Barić I, Rados M, Mudd SH (2010) J Inherit Metab Dis 33(6): 705-13
    › Primary publication · 20852937 (PubMed)
  20. Enzymatic activity of methionine adenosyltransferase variants identified in patients with persistent hypermethioninemia. Fernández-Irigoyen J, Santamaría E, Chien YH, Hwu WL, Korman SH, Faghfoury H, Schulze A, Hoganson GE, Stabler SP, Allen RH, Wagner C, Mudd SH, Corrales FJ (2010) Mol Genet Metab 101(2-3): 172-7
    › Primary publication · 20675163 (PubMed)
  21. Fatty liver and fibrosis in glycine N-methyltransferase knockout mice is prevented by nicotinamide. Varela-Rey M, Martínez-López N, Fernández-Ramos D, Embade N, Calvisi DF, Woodhoo A, Rodríguez J, Fraga MF, Julve J, Rodríguez-Millán E, Frades I, Torres L, Luka Z, Wagner C, Esteller M, Lu SC, Martínez-Chantar ML, Mato JM (2010) Hepatology 52(1): 105-14
    › Primary publication · 20578266 (PubMed) · PMC2921576 (PubMed Central)
  22. Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples. Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M (2010) Proteomics Clin Appl 4(4): 416-25
    › Primary publication · 20535238 (PubMed) · PMC2882112 (PubMed Central)
  23. HuR/methyl-HuR and AUF1 regulate the MAT expressed during liver proliferation, differentiation, and carcinogenesis. Vázquez-Chantada M, Fernández-Ramos D, Embade N, Martínez-Lopez N, Varela-Rey M, Woodhoo A, Luka Z, Wagner C, Anglim PP, Finnell RH, Caballería J, Laird-Offringa IA, Gorospe M, Lu SC, Mato JM, Martínez-Chantar ML (2010) Gastroenterology 138(5): 1943-53
    › Primary publication · 20102719 (PubMed) · PMC2860011 (PubMed Central)
  24. The role of stem cells/progenitor cells in liver carcinogenesis in glycine N-methyltransferase deficient mice. Martinez-Chantar ML, Lu SC, Mato JM, Luka Z, Wagner C, French BA, French SW (2010) Exp Mol Pathol 88(2): 234-7
    › Primary publication · 20080087 (PubMed) · PMC3030975 (PubMed Central)
  25. Impaired liver regeneration in mice lacking glycine N-methyltransferase. Varela-Rey M, Fernández-Ramos D, Martínez-López N, Embade N, Gómez-Santos L, Beraza N, Vázquez-Chantada M, Rodríguez J, Luka Z, Wagner C, Lu SC, Martínez-Chantar ML, Mato JM (2009) Hepatology 50(2): 443-52
    › Primary publication · 19582817 (PubMed) · PMC2805126 (PubMed Central)
  26. Glycine N-methyltransferase and regulation of S-adenosylmethionine levels. Luka Z, Mudd SH, Wagner C (2009) J Biol Chem 284(34): 22507-11
    › Primary publication · 19483083 (PubMed) · PMC2755656 (PubMed Central)
  27. Hepatocellular carcinoma in GNMT-/- mice. Wagner C, Luka Z, Mato JM (2009) Toxicol Appl Pharmacol 237(2): 246; author reply 247
    › Primary publication · 19345238 (PubMed)
  28. Dietary B vitamin and methionine intakes and plasma folate are not associated with colorectal cancer risk in Chinese women. Shrubsole MJ, Yang G, Gao YT, Chow WH, Shu XO, Cai Q, Rothman N, Gao J, Wagner C, Zheng W (2009) Cancer Epidemiol Biomarkers Prev 18(3): 1003-6
    › Primary publication · 19240230 (PubMed) · PMC2677023 (PubMed Central)
  29. Changes in S-adenosylmethionine and GSH homeostasis during endotoxemia in mice. Ko K, Yang H, Noureddin M, Iglesia-Ara A, Xia M, Wagner C, Luka Z, Mato JM, Lu SC (2008) Lab Invest 88(10): 1121-9
    › Primary publication · 18695670 (PubMed) · PMC4467989 (PubMed Central)
  30. Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate. Luka Z, Loukachevitch LV, Wagner C (2008) Biochim Biophys Acta 1784(9): 1342-6
    › Primary publication · 18501206 (PubMed) · PMC2576408 (PubMed Central)
  31. Plasma S-adenosylhomocysteine versus homocysteine as a marker for vascular disease. Wagner C, Koury MJ (2008) J Nutr 138(5): 980; author reply 981
    › Primary publication · 18424611 (PubMed)
  32. Loss of the glycine N-methyltransferase gene leads to steatosis and hepatocellular carcinoma in mice. Martínez-Chantar ML, Vázquez-Chantada M, Ariz U, Martínez N, Varela M, Luka Z, Capdevila A, Rodríguez J, Aransay AM, Matthiesen R, Yang H, Calvisi DF, Esteller M, Fraga M, Lu SC, Wagner C, Mato JM (2008) Hepatology 47(4): 1191-9
    › Primary publication · 18318442 (PubMed) · PMC2405897 (PubMed Central)
  33. S-Adenosylhomocysteine: a better indicator of vascular disease than homocysteine? Wagner C, Koury MJ (2007) Am J Clin Nutr 86(6): 1581-5
    › Primary publication · 18065573 (PubMed)
  34. Destabilization of human glycine N-methyltransferase by H176N mutation. Luka Z, Pakhomova S, Luka Y, Newcomer ME, Wagner C (2007) Protein Sci 16(9): 1957-64
    › Primary publication · 17660255 (PubMed) · PMC2206963 (PubMed Central)
  35. A simple rapid immunoassay for S-adenosylhomocysteine in plasma. Capdevila A, Burk RF, Freedman J, Frantzen F, Alfheim I, Wagner C (2007) J Nutr Biochem 18(12): 827-31
    › Primary publication · 17509856 (PubMed) · PMC2196216 (PubMed Central)
  36. Prevention of brain disease from severe 5,10-methylenetetrahydrofolate reductase deficiency. Strauss KA, Morton DH, Puffenberger EG, Hendrickson C, Robinson DL, Wagner C, Stabler SP, Allen RH, Chwatko G, Jakubowski H, Niculescu MD, Mudd SH (2007) Mol Genet Metab 91(2): 165-75
    › Primary publication · 17409006 (PubMed)
  37. Methyl balance and transmethylation fluxes in humans. Mudd SH, Brosnan JT, Brosnan ME, Jacobs RL, Stabler SP, Allen RH, Vance DE, Wagner C (2007) Am J Clin Nutr 85(1): 19-25
    › Primary publication · 17209172 (PubMed)
  38. 5-methyltetrahydrofolate is bound in intersubunit areas of rat liver folate-binding protein glycine N-methyltransferase. Luka Z, Pakhomova S, Loukachevitch LV, Egli M, Newcomer ME, Wagner C (2007) J Biol Chem 282(6): 4069-75
    › Primary publication · 17158459 (PubMed)
  39. A glycine N-methyltransferase knockout mouse model for humans with deficiency of this enzyme. Luka Z, Capdevila A, Mato JM, Wagner C (2006) Transgenic Res 15(3): 393-7
    › Primary publication · 16779654 (PubMed) · PMC2792375 (PubMed Central)
  40. S-adenosylhomocysteine hydrolase deficiency in a 26-year-old man. Buist NR, Glenn B, Vugrek O, Wagner C, Stabler S, Allen RH, Pogribny I, Schulze A, Zeisel SH, Barić I, Mudd SH (2006) J Inherit Metab Dis 29(4): 538-45
    › Primary publication · 16736098 (PubMed) · PMC2430673 (PubMed Central)
  41. Identification of phosphorylation sites in glycine N-methyltransferase from rat liver. Luka Z, Ham AJ, Norris JL, Yeo EJ, Yermalitsky V, Glenn B, Caprioli RM, Liebler DC, Wagner C (2006) Protein Sci 15(4): 785-94
    › Primary publication · 16522797 (PubMed) · PMC2242492 (PubMed Central)
  42. S-Adenosylhomocysteine hydrolase deficiency: a second patient, the younger brother of the index patient, and outcomes during therapy. Barić I, Cuk M, Fumić K, Vugrek O, Allen RH, Glenn B, Maradin M, Pazanin L, Pogribny I, Rados M, Sarnavka V, Schulze A, Stabler S, Wagner C, Zeisel SH, Mudd SH (2005) J Inherit Metab Dis 28(6): 885-902
    › Primary publication · 16435181 (PubMed) · PMC2441944 (PubMed Central)
  43. Relationship between plasma S-adenosylhomocysteine concentration and glomerular filtration rate in children. Jabs K, Koury MJ, Dupont WD, Wagner C (2006) Metabolism 55(2): 252-7
    › Primary publication · 16423634 (PubMed)
  44. Homocysteine synthesis is elevated but total remethylation is unchanged by the methylenetetrahydrofolate reductase 677C->T polymorphism and by dietary folate restriction in young women. Davis SR, Quinlivan EP, Shelnutt KP, Ghandour H, Capdevila A, Coats BS, Wagner C, Shane B, Selhub J, Bailey LB, Shuster JJ, Stacpoole PW, Gregory JF (2005) J Nutr 135(5): 1045-50
    › Primary publication · 15867279 (PubMed)
  45. The methylenetetrahydrofolate reductase 677C->T polymorphism and dietary folate restriction affect plasma one-carbon metabolites and red blood cell folate concentrations and distribution in women. Davis SR, Quinlivan EP, Shelnutt KP, Maneval DR, Ghandour H, Capdevila A, Coats BS, Wagner C, Selhub J, Bailey LB, Shuster JJ, Stacpoole PW, Gregory JF (2005) J Nutr 135(5): 1040-4
    › Primary publication · 15867278 (PubMed)
  46. Creatine metabolism in combined methylmalonic aciduria and homocystinuria. Bodamer OA, Sahoo T, Beaudet AL, O'Brien WE, Bottiglieri T, Stöckler-Ipsiroglu S, Wagner C, Scaglia F (2005) Ann Neurol 57(4): 557-60
    › Primary publication · 15786446 (PubMed)
  47. The Arabidopsis HOMOLOGY-DEPENDENT GENE SILENCING1 gene codes for an S-adenosyl-L-homocysteine hydrolase required for DNA methylation-dependent gene silencing. Rocha PS, Sheikh M, Melchiorre R, Fagard M, Boutet S, Loach R, Moffatt B, Wagner C, Vaucheret H, Furner I (2005) Plant Cell 17(2): 404-17
    › Primary publication · 15659630 (PubMed) · PMC548815 (PubMed Central)
  48. Glycine N-methyltransferases: a comparison of the crystal structures and kinetic properties of recombinant human, mouse and rat enzymes. Pakhomova S, Luka Z, Grohmann S, Wagner C, Newcomer ME (2004) Proteins 57(2): 331-7
    › Primary publication · 15340920 (PubMed)
  49. S-adenosylhomocysteine hydrolase deficiency in a human: a genetic disorder of methionine metabolism. Baric I, Fumic K, Glenn B, Cuk M, Schulze A, Finkelstein JD, James SJ, Mejaski-Bosnjak V, Pazanin L, Pogribny IP, Rados M, Sarnavka V, Scukanec-Spoljar M, Allen RH, Stabler S, Uzelac L, Vugrek O, Wagner C, Zeisel S, Mudd SH (2004) Proc Natl Acad Sci U S A 101(12): 4234-9
    › Primary publication · 15024124 (PubMed) · PMC384724 (PubMed Central)
  50. Glycine N -methyltransferase deficiency: a new patient with a novel mutation. Augoustides-Savvopoulou P, Luka Z, Karyda S, Stabler SP, Allen RH, Patsiaoura K, Wagner C, Mudd SH (2003) J Inherit Metab Dis 26(8): 745-59
    › Primary publication · 14739680 (PubMed)
  51. Effect of naturally occurring mutations in human glycine N-methyltransferase on activity and conformation. Luka Z, Wagner C (2003) Biochem Biophys Res Commun 312(4): 1067-72
    › Primary publication · 14651980 (PubMed)
  52. Human glycine N-methyltransferase is unfolded by urea through a compact monomer state. Luka Z, Wagner C (2003) Arch Biochem Biophys 420(1): 153-60
    › Primary publication · 14622985 (PubMed)
  53. Maternal methionine adenosyltransferase I/III deficiency: reproductive outcomes in a woman with four pregnancies. Mudd SH, Tangerman A, Stabler SP, Allen RH, Wagner C, Zeisel SH, Levy HL (2003) J Inherit Metab Dis 26(5): 443-58
    › Primary publication · 14518826 (PubMed)
  54. Bacterial fermentation of dimethyl-beta-propiothetin. WAGNER C, STADTMAN ER (1962) Arch Biochem Biophys : 331-6
    › Primary publication · 14004329 (PubMed)
  55. Infantile hypermethioninemia and hyperhomocysteinemia due to high methionine intake: a diagnostic trap. Harvey Mudd S, Braverman N, Pomper M, Tezcan K, Kronick J, Jayakar P, Garganta C, Ampola MG, Levy HL, McCandless SE, Wiltse H, Stabler SP, Allen RH, Wagner C, Borschel MW (2003) Mol Genet Metab 79(1): 6-16
    › Primary publication · 12765841 (PubMed)
  56. Expression and purification of glycine N-methyltransferases in Escherichia coli. Luka Z, Wagner C (2003) Protein Expr Purif 28(2): 280-6
    › Primary publication · 12699692 (PubMed)
  57. Insertional inactivation of the methionine s-methyltransferase gene eliminates the s-methylmethionine cycle and increases the methylation ratio. Kocsis MG, Ranocha P, Gage DA, Simon ES, Rhodes D, Peel GJ, Mellema S, Saito K, Awazuhara M, Li C, Meeley RB, Tarczynski MC, Wagner C, Hanson AD (2003) Plant Physiol 131(4): 1808-15
    › Primary publication · 12692340 (PubMed) · PMC166937 (PubMed Central)
  58. Elevated plasma total homocysteine in severe methionine adenosyltransferase I/III deficiency. Stabler SP, Steegborn C, Wahl MC, Oliveriusova J, Kraus JP, Allen RH, Wagner C, Mudd SH (2002) Metabolism 51(8): 981-8
    › Primary publication · 12145770 (PubMed)
  59. Adverse event associated with methionine loading test: a case report. Cottington EM, LaMantia C, Stabler SP, Allen RH, Tangerman A, Wagner C, Zeisel SH, Mudd SH (2002) Arterioscler Thromb Vasc Biol 22(6): 1046-50
    › Primary publication · 12067919 (PubMed)
  60. William J. Darby, 1913-2001. Sandstead HH, Wagner C (2002) J Nutr 132(6): 1103-6
    › Primary publication · 12042417 (PubMed)
  61. Mutations in human glycine N-methyltransferase give insights into its role in methionine metabolism. Luka Z, Cerone R, Phillips JA, Mudd HS, Wagner C (2002) Hum Genet 110(1): 68-74
    › Primary publication · 11810299 (PubMed)
  62. Impact of extracellular folate levels on global gene expression. Jhaveri MS, Wagner C, Trepel JB (2001) Mol Pharmacol 60(6): 1288-95
    › Primary publication · 11723236 (PubMed)
  63. Plasma S-adenosylhomocysteine is a more sensitive indicator of cardiovascular disease than plasma homocysteine. Kerins DM, Koury MJ, Capdevila A, Rana S, Wagner C (2001) Am J Clin Nutr 74(6): 723-9
    › Primary publication · 11722952 (PubMed)
  64. Glycine N-methyltransferase deficiency: a novel inborn error causing persistent isolated hypermethioninaemia. Mudd SH, Cerone R, Schiaffino MC, Fantasia AR, Minniti G, Caruso U, Lorini R, Watkins D, Matiaszuk N, Rosenblatt DS, Schwahn B, Rozen R, LeGros L, Kotb M, Capdevila A, Luka Z, Finkelstein JD, Tangerman A, Stabler SP, Allen RH, Wagner C (2001) J Inherit Metab Dis 24(4): 448-64
    › Primary publication · 11596649 (PubMed)
  65. On the role of conserved histidine 106 in 10-formyltetrahydrofolate dehydrogenase catalysis: connection between hydrolase and dehydrogenase mechanisms. Krupenko SA, Vlasov AP, Wagner C (2001) J Biol Chem 276(26): 24030-7
    › Primary publication · 11320079 (PubMed)
  66. Inhibition of liver methionine adenosyltransferase gene expression by 3-methylcolanthrene: protective effect of S-adenosylmethionine. Carretero MV, Latasa MU, Garcia-Trevijano ER, Corrales FJ, Wagner C, Mato JM, Avila MA (2001) Biochem Pharmacol 61(9): 1119-28
    › Primary publication · 11301045 (PubMed)
  67. Isolated hypermethioninemia: measurements of S-adenosylmethionine and choline. Mudd SH, Jenden DJ, Capdevila A, Roch M, Levy HL, Wagner C (2000) Metabolism 49(12): 1542-7
    › Primary publication · 11145114 (PubMed)
  68. Inhibition of glycine N-methyltransferase by 5-methyltetrahydrofolate pentaglutamate. Yeo EJ, Briggs WT, Wagner C (1999) J Biol Chem 274(53): 37559-64
    › Primary publication · 10608809 (PubMed)
  69. Aspartate 142 is involved in both hydrolase and dehydrogenase catalytic centers of 10-formyltetrahydrofolate dehydrogenase. Krupenko SA, Wagner C (1999) J Biol Chem 274(50): 35777-84
    › Primary publication · 10585460 (PubMed)
  70. Measurement of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent isoindoles. Capdevila A, Wagner C (1998) Anal Biochem 264(2): 180-4
    › Primary publication · 9866680 (PubMed)
  71. Overexpression of functional hydrolase domain of rat liver 10-formyltetrahydrofolate dehydrogenase in Escherichia coli. Krupenko SA, Wagner C (1998) Protein Expr Purif 14(1): 146-52
    › Primary publication · 9758763 (PubMed)
  72. Crystal structure of apo-glycine N-methyltransferase (GNMT). Pattanayek R, Newcomer ME, Wagner C (1998) Protein Sci 7(6): 1326-31
    › Primary publication · 9655336 (PubMed) · PMC2144038 (PubMed Central)
  73. Transport of rat liver glycine N-methyltransferase into rat liver nuclei. Krupenko NI, Wagner C (1997) J Biol Chem 272(43): 27140-6
    › Primary publication · 9341155 (PubMed)
  74. Pancreatic exocrine secretion is blocked by inhibitors of methylation. Capdevila A, Decha-Umphai W, Song KH, Borchardt RT, Wagner C (1997) Arch Biochem Biophys 345(1): 47-55
    › Primary publication · 9281310 (PubMed)
  75. Transport of S-adenosylmethionine in isolated rat liver mitochondria. Horne DW, Holloway RS, Wagner C (1997) Arch Biochem Biophys 343(2): 201-6
    › Primary publication · 9224731 (PubMed)
  76. Domain structure of rat 10-formyltetrahydrofolate dehydrogenase. Resolution of the amino-terminal domain as 10-formyltetrahydrofolate hydrolase. Krupenko SA, Wagner C, Cook RJ (1997) J Biol Chem 272(15): 10273-8
    › Primary publication · 9092578 (PubMed)
  77. Expression, purification, and properties of the aldehyde dehydrogenase homologous carboxyl-terminal domain of rat 10-formyltetrahydrofolate dehydrogenase. Krupenko SA, Wagner C, Cook RJ (1997) J Biol Chem 272(15): 10266-72
    › Primary publication · 9092577 (PubMed)
  78. Covalent binding of acetaminophen to N-10-formyltetrahydrofolate dehydrogenase in mice. Pumford NR, Halmes NC, Martin BM, Cook RJ, Wagner C, Hinson JA (1997) J Pharmacol Exp Ther 280(1): 501-5
    › Primary publication · 8996234 (PubMed)
  79. Symposium on the subcellular compartmentation of folate metabolism. Wagner C (1996) J Nutr 126(4 Suppl): 1228S-34S
    › Primary publication · 8642461 (PubMed)
  80. Baculovirus expression and purification of rat 10-formyltetrahydrofolate dehydrogenase. Krupenko SA, Horstman DA, Wagner C, Cook RJ (1995) Protein Expr Purif 6(4): 457-64
    › Primary publication · 8527931 (PubMed)
  81. Hepatic one-carbon metabolism in early folate deficiency in rats. Balaghi M, Horne DW, Wagner C (1993) Biochem J : 145-9
    › Primary publication · 8471033 (PubMed) · PMC1132493 (PubMed Central)
  82. Pancreatic one-carbon metabolism in early folate deficiency in rats. Balaghi M, Horne DW, Woodward SC, Wagner C (1993) Am J Clin Nutr 58(2): 198-203
    › Primary publication · 8338048 (PubMed)
  83. DNA methylation in folate deficiency: use of CpG methylase. Balaghi M, Wagner C (1993) Biochem Biophys Res Commun 193(3): 1184-90
    › Primary publication · 8323540 (PubMed)
  84. Tissue distribution of glycine N-methyltransferase, a major folate-binding protein of liver. Yeo EJ, Wagner C (1994) Proc Natl Acad Sci U S A 91(1): 210-4
    › Primary publication · 8278367 (PubMed) · PMC42916 (PubMed Central)
  85. 10-Formyltetrahydrofolate dehydrogenase: identification of the natural folate ligand, covalent labeling, and partial tryptic digestion. Wagner C, Briggs WT, Horne DW, Cook RJ (1995) Arch Biochem Biophys 316(1): 141-7
    › Primary publication · 7840609 (PubMed)
  86. Cysteine 707 is involved in the dehydrogenase activity site of rat 10-formyltetrahydrofolate dehydrogenase. Krupenko SA, Wagner C, Cook RJ (1995) J Biol Chem 270(2): 519-22
    › Primary publication · 7822273 (PubMed)
  87. Recombinant 10-formyltetrahydrofolate dehydrogenase catalyses both dehydrogenase and hydrolase reactions utilizing the synthetic substrate 10-formyl-5,8-dideazafolate. Krupenko SA, Wagner C, Cook RJ (1995) Biochem J : 651-5
    › Primary publication · 7702556 (PubMed) · PMC1136571 (PubMed Central)
  88. Enzymatic activities of rat liver cytosol 10-formyltetrahydrofolate dehydrogenase. Cook RJ, Wagner C (1995) Arch Biochem Biophys 321(2): 336-44
    › Primary publication · 7646059 (PubMed)
  89. Folate deficiency inhibits pancreatic amylase secretion in rats. Balaghi M, Wagner C (1995) Am J Clin Nutr 61(1): 90-6
    › Primary publication · 7529961 (PubMed)
  90. Enzymic synthesis of (d)-L-5-methyltetrahydropteroylglutamate of high specific radioactivity. Horne DW, Briggs WT, Wagner C (1980) Methods Enzymol : 545-7
    › Primary publication · 7374497 (PubMed)
  91. Purification and characterization of a folate binding protein from rat liver cytosol. Suzuki N, Wagner C (1980) Arch Biochem Biophys 199(1): 236-48
    › Primary publication · 7356330 (PubMed)
  92. High-pressure liquid chromatographic separation of the naturally occurring folic acid monoglutamate derivatives. Horne DW, Briggs WT, Wagner C (1981) Anal Biochem 116(2): 393-7
    › Primary publication · 7316172 (PubMed)
  93. Measurement of a folate binding protein from rat liver cytosol by radioimmunoassay. Cook RJ, Wagner C (1981) Arch Biochem Biophys 208(2): 358-64
    › Primary publication · 7259194 (PubMed)
  94. Purification and partial characterization of rat liver folate binding protein: cytosol I. Cook RJ, Wagner C (1982) Biochemistry 21(18): 4427-34
    › Primary publication · 6897000 (PubMed)
  95. Properties of folic acid gamma-glutamyl hydrolase (conjugase) in rat bile and plasma. Horne DW, Krumdieck CL, Wagner C (1981) J Nutr 111(3): 442-9
    › Primary publication · 6894161 (PubMed)
  96. Cellular folate binding proteins; function and significance. Wagner C (1982) Annu Rev Nutr : 229-48
    › Primary publication · 6821190 (PubMed)
  97. Glycine N-methyltransferase is a folate binding protein of rat liver cytosol. Cook RJ, Wagner C (1984) Proc Natl Acad Sci U S A 81(12): 3631-4
    › Primary publication · 6587377 (PubMed) · PMC345272 (PubMed Central)
  98. Identification of the covalently bound flavin of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria. Cook RJ, Misono KS, Wagner C (1984) J Biol Chem 259(20): 12475-80
    › Primary publication · 6490627 (PubMed)
  99. Covalent binding of folic acid to dimethylglycine dehydrogenase. Wagner C, Briggs WT, Cook RJ (1984) Arch Biochem Biophys 233(2): 457-61
    › Primary publication · 6486795 (PubMed)
  100. Identification of the folate-binding proteins of rat liver mitochondria as dimethylglycine dehydrogenase and sarcosine dehydrogenase. Flavoprotein nature and enzymatic properties of the purified proteins. Wittwer AJ, Wagner C (1981) J Biol Chem 256(8): 4109-15
    › Primary publication · 6163778 (PubMed)
  101. Identification of the folate-binding proteins of rat liver mitochondria as dimethylglycine dehydrogenase and sarcosine dehydrogenase. Purification and folate-binding characteristics. Wittwer AJ, Wagner C (1981) J Biol Chem 256(8): 4102-8
    › Primary publication · 6163777 (PubMed)
  102. Identification of folate binding protein of mitochondria as dimethylglycine dehydrogenase. Wittwer AJ, Wagner C (1980) Proc Natl Acad Sci U S A 77(8): 4484-8
    › Primary publication · 6159630 (PubMed) · PMC349868 (PubMed Central)
  103. Preparation and properties of trimethylsulfonium-tetrahydrofolate methyltransferase. Wagner C, Lusty SM, Kung HF, Rogers NL (1967) J Biol Chem 242(6): 1287-93
    › Primary publication · 6023571 (PubMed)
  104. Spectral absorption characteristics of human methemalbumin. Daniels CA, Wagner C, Hartmann RC, Jenkins DE (1967) Am J Clin Pathol 47(5): 631-7
    › Primary publication · 6022994 (PubMed)
  105. Trimethylsulfonium-tetrahydrofolate methyltransferase, a novel enzyme in the utilization of 1 carbon units. Wagner C, Lusty SM, Kung HF, Rogers NL (1966) J Biol Chem 241(8): 1923-5
    › Primary publication · 5945864 (PubMed)
  106. Tryptophan degradation in the developing chick. Wagner C, Payne NA, Briggs W (1969) Exp Cell Res 55(3): 330-8
    › Primary publication · 5815055 (PubMed)
  107. Gamma-glutamylmethylamide. A new intermediate in the metabolism of methylamine. Kung HF, Wagner C (1969) J Biol Chem 244(15): 4136-40
    › Primary publication · 5800436 (PubMed)
  108. Oxidation of C-1 compounds by Pseudomonas sp. MS. Kung HF, Wagner C (1970) Biochem J 116(3): 357-65
    › Primary publication · 5435683 (PubMed) · PMC1185373 (PubMed Central)
  109. Malabsorption of thiamin in folate-deficient rats. Howard L, Wagner C, Schenker S (1974) J Nutr 104(8): 1024-32
    › Primary publication · 4853073 (PubMed)
  110. The utilization of formate by human erythrocytes. Wagner C, Levitch ME (1973) Biochim Biophys Acta 304(3): 623-33
    › Primary publication · 4726849 (PubMed)
  111. The metabolism of lactate and pyruvate by Pseudomonas AM1. Salem AR, Wagner C, Hacking AJ, Quayle JR (1973) J Gen Microbiol 76(2): 375-88
    › Primary publication · 4723074 (PubMed)
  112. The uptake of acetate by Escherichia coli w. Wagner C, Odom R, Briggs WT (1972) Biochem Biophys Res Commun 47(5): 1036-43
    › Primary publication · 4555245 (PubMed)
  113. Decreased aspartate aminotransferase ("SGOT") activity in serum of uremic patients. Warnock LG, Stone WJ, Wagner C (1974) Clin Chem 20(9): 1213-6
    › Primary publication · 4412711 (PubMed)
  114. Carbon assimilation pathways during growth of Pseudomonas AM1 on methylamine and Pseudomonas MS on methylamine and trimethylsulphonium salts. Wagner C, Quayle JR (1972) J Gen Microbiol 72(3): 485-91
    › Primary publication · 4405379 (PubMed)
  115. The enzymatic synthesis of N-methylalanine. Kung HF, Wagner C (1970) Biochim Biophys Acta 201(3): 513-6
    › Primary publication · 4314464 (PubMed)
  116. Regulation of enzymes involved in the conversion of tryptophan to nicotinamide adenine dinucleotide in a colorless strain of Xanthomonas pruni. Brown AT, Wagner C (1970) J Bacteriol 101(2): 456-63
    › Primary publication · 4313053 (PubMed) · PMC284928 (PubMed Central)
  117. Regulation of tryptophan pyrrolase activity in Xanthomonas pruni. Wagner C, Brown AT (1970) J Bacteriol 104(1): 90-7
    › Primary publication · 4248693 (PubMed) · PMC248187 (PubMed Central)
  118. A simple method for the synthesis of nicotinic acid mononucleotide. Wagner C (1968) Anal Biochem 25(1): 472-6
    › Primary publication · 4236516 (PubMed)
  119. High molecular weight complexes of folic acid in mammalian tissues. Zamierowski M, Wagner C (1974) Biochem Biophys Res Commun 60(1): 81-7
    › Primary publication · 4213566 (PubMed)
  120. Quantitative determination of secondary amines: measurement of N-methylalanine. Lin CM, Wagner C (1974) Anal Biochem 60(1): 278-84
    › Primary publication · 4137152 (PubMed)
  121. Kynurenine formamidase. Purification and characterization of the adult chicken liver enzyme and immunochemical analyses of the enzyme of developing chicks. Bailey CG, Wagner C (1974) J Biol Chem 249(14): 4439-44
    › Primary publication · 4135584 (PubMed)
  122. The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria. Cook RJ, Misono KS, Wagner C (1985) J Biol Chem 260(24): 12998-3002
    › Primary publication · 4055729 (PubMed)
  123. Folate-binding proteins. Wagner C (1985) Nutr Rev 43(10): 293-9
    › Primary publication · 3909007 (PubMed)
  124. Inhibition of glycine N-methyltransferase activity by folate derivatives: implications for regulation of methyl group metabolism. Wagner C, Briggs WT, Cook RJ (1985) Biochem Biophys Res Commun 127(3): 746-52
    › Primary publication · 3838667 (PubMed)
  125. Dimethylglycine dehydrogenase and sarcosine dehydrogenase: mitochondrial folate-binding proteins from rat liver. Cook RJ, Wagner C (1986) Methods Enzymol : 255-60
    › Primary publication · 3702694 (PubMed)
  126. Purification of rat liver folate-binding protein: cytosol I. Cook RJ, Wagner C (1986) Methods Enzymol : 251-5
    › Primary publication · 3517563 (PubMed)
  127. Effect of human milk folate binding protein on folate intestinal transport. Said HM, Horne DW, Wagner C (1986) Arch Biochem Biophys 251(1): 114-20
    › Primary publication · 2878641 (PubMed)
  128. Effect of dietary methyl group deficiency on folate metabolism in rats. Horne DW, Cook RJ, Wagner C (1989) J Nutr 119(4): 618-21
    › Primary publication · 2784833 (PubMed)
  129. Phosphorylation modulates the activity of glycine N-methyltransferase, a folate binding protein. In vitro phosphorylation is inhibited by the natural folate ligand. Wagner C, Decha-Umphai W, Corbin J (1989) J Biol Chem 264(16): 9638-42
    › Primary publication · 2722853 (PubMed)
  130. Effect of dietary methyl group deficiency on one-carbon metabolism in rats. Cook RJ, Horne DW, Wagner C (1989) J Nutr 119(4): 612-7
    › Primary publication · 2703919 (PubMed)
  131. Measurement of dimethylglycine in biological fluids. Porter DH, Lin M, Wagner C (1985) Anal Biochem 151(2): 299-303
    › Primary publication · 2420226 (PubMed)
  132. Enzymatic properties of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver. Porter DH, Cook RJ, Wagner C (1985) Arch Biochem Biophys 243(2): 396-407
    › Primary publication · 2417560 (PubMed)
  133. Pipecolic acid biosynthesis in Rhizoctonia leguminicola. II. Saccharopine oxidase: a unique flavin enzyme involved in pipecolic acid biosynthesis. Wickwire BM, Wagner C, Broquist HP (1990) J Biol Chem 265(25): 14748-53
    › Primary publication · 2394693 (PubMed)
  134. Isolation and characterization of cDNA clones for rat liver 10-formyltetrahydrofolate dehydrogenase. Cook RJ, Lloyd RS, Wagner C (1991) J Biol Chem 266(8): 4965-73
    › Primary publication · 1848231 (PubMed)
  135. Methyl group metabolism in the pancreas of folate-deficient rats. Balaghi M, Wagner C (1992) J Nutr 122(7): 1391-6
    › Primary publication · 1619467 (PubMed)
  136. Purification and properties of pancreatic glycine N-methyltransferase. Yeo EJ, Wagner C (1992) J Biol Chem 267(34): 24669-74
    › Primary publication · 1332963 (PubMed)
  137. Folate transport in the choroid plexus. Chen CP, Wagner C (1975) Life Sci 16(10): 1571-81
    › Primary publication · 1195980 (PubMed)
  138. Vitamin B6 deficiency in uremia. Stone WJ, Warnock LG, Wagner C (1975) Am J Clin Nutr 28(9): 950-7
    › Primary publication · 1163479 (PubMed)
  139. A functional, active transport system for methotrexate in freshly isolated hepatocytes. Horne DW, Briggs WT, Wagner C (1976) Biochem Biophys Res Commun 68(1): 70-6
    › Primary publication · 1082335 (PubMed)
  140. Abnormal regulation of plasma pyridoxal 5'-phosphate in patients with liver disease. Mitchell D, Wagner C, Stone WJ, Wilkinson GR, Schenker S (1976) Gastroenterology 71(6): 1043-9
    › Primary publication · 992265 (PubMed)
  141. Effect of folacin deficiency on folacin-binding proteins in the rat. Zamierowski MM, Wagner C (1977) J Nutr 107(10): 1937-45
    › Primary publication · 903836 (PubMed)
  142. Increased plasma clearance of pyridoxal 5'-phosphate in vitamin B6-deficient uremic man. Spannuth CL, Warnock LG, Wagner C, Stone WJ (1977) J Lab Clin Med 90(4): 632-7
    › Primary publication · 903695 (PubMed)
  143. Identification of folate binding proteins in rat liver. Zamierowski MM, Wagner C (1977) J Biol Chem 252(3): 933-8
    › Primary publication · 838705 (PubMed)
  144. Ethanol stimulates 5-methyltetrahydrofolate accumulation in isolated rat liver cells. Horne DW, Briggs WT, Wagner C (1978) Biochem Pharmacol 27(16): 2069-74
    › Primary publication · 718731 (PubMed)
  145. Transport of 5-methyltetrahydrofolic acid and folic acid in freshly isolated hepatocytes. Horne DW, Briggs WT, Wagner C (1978) J Biol Chem 253(10): 3529-35
    › Primary publication · 649587 (PubMed)
  146. The determination of thiamin pyrophosphate in blood and other tissues, and its correlation with erythrocyte transketolase activity. Warnock LG, Prudhomme CR, Wagner C (1978) J Nutr 108(3): 421-7
    › Primary publication · 627916 (PubMed)
  147. Enzymatic preparation of high specific activity radiolabeled (I)-L-5-methyltetrahydropteroylglutamate. Horne DW, Briggs WT, Wagner C (1977) Anal Biochem 83(2): 615-21
    › Primary publication · 603044 (PubMed)
  148. Studies on the transport mechanism of 5-methyltetrahydrofolic acid in freshly isolated hepatocytes: effect of ethanol. Horne DW, Briggs WT, Wagner C (1979) Arch Biochem Biophys 196(2): 557-65
    › Primary publication · 485165 (PubMed)
  149. The peracid cleavage of 5-methyltetrahydrofolic acid at the C9-N10 bridge. Baugh CM, Braverman EB, Nair MG, Horne DW, Briggs WT, Wagner C (1979) Anal Biochem 92(2): 366-9
    › Primary publication · 443537 (PubMed)
  150. Enzymes involved in the assimilation of one-carbon units by Pseudomonas MS. Wagner C, Levitch ME (1975) J Bacteriol 122(3): 905-10
    › Primary publication · 238950 (PubMed) · PMC246141 (PubMed Central)
  151. Purification and characterization of N-methylalanine dehydrogenase. Lin MC, Wagner C (1975) J Biol Chem 250(10): 3746-51
    › Primary publication · 236301 (PubMed)