Ryan Stark
Last active: 8/6/2020


My current work focuses on the inflammatory effect of infection on an often overlooked component of innate immunity, the endothelium. Specifically, I am interested how endothelial cells are affected during infection, with specific focus on endothelial nitric oxide synthase dysfunction. Furthermore, I am exploring how priming of the endothelium with a TLR4 agonist, monophosphoryl lipid a (MPLA), alters the pro-inflammatory responses of activated endothelial cells in a models of burn-injury and acquired infections. The long term goal of this work is to understand the mechanisms of endothelial dysfunction during infections and find modalities to mitigate the effects of infection on endothelial activation through the use of immunomodulators.


The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. TNFα and Reactive Oxygen Signaling in Vascular Smooth Muscle Cells in Hypertension and Atherosclerosis. Lamb FS, Choi H, Miller MR, Stark RJ (2020) Am J Hypertens 33(10): 902-913
    › Primary publication · 32498083 (PubMed)
  2. Neuronal ASIC1A As a Cerebral pH Sensor: Bringing the Flow. Stark RJ, Choi H, Lamb FS (2019) Circ Res 125(10): 921-923
    › Primary publication · 31647772 (PubMed) · PMC6993881 (PubMed Central)
  3. Endothelial-Dependent Vasomotor Dysfunction in Infants After Cardiopulmonary Bypass. Krispinsky LT, Stark RJ, Parra DA, Luan L, Bichell DP, Pietsch JB, Lamb FS (2020) Pediatr Crit Care Med 21(1): 42-49
    › Primary publication · 31246738 (PubMed)
  4. Monophosphoryl Lipid a Attenuates Multiorgan Dysfunction During Post-Burn Pseudomonas Aeruginosa Pneumonia in Sheep. Fukuda S, Ihara K, Bohannon JK, Hernandez A, Patil NK, Luan L, Stothers C, Stark R, Prough DS, Herndon DN, Sherwood ER, Enkhbaatar P (2020) Shock 53(3): 307-316
    › Primary publication · 31045990 (PubMed) · PMC6937402 (PubMed Central)
  5. Late immune consequences of combat trauma: a review of trauma-related immune dysfunction and potential therapies. Thompson KB, Krispinsky LT, Stark RJ (2019) Mil Med Res 6(1): 11
    › Primary publication · 31014397 (PubMed) · PMC6480837 (PubMed Central)
  6. Toll-like receptor 3-mediated inflammation by p38 is enhanced by endothelial nitric oxide synthase knockdown. Koch SR, Choi H, Mace EH, Stark RJ (2019) Cell Commun Signal 17(1): 33
    › Primary publication · 30987646 (PubMed) · PMC6466662 (PubMed Central)
  7. Apoptosis signal-regulating kinase 1 activation by Nox1-derived oxidants is required for TNFα receptor endocytosis. Choi H, Stark RJ, Raja BS, Dikalova A, Lamb FS (2019) Am J Physiol Heart Circ Physiol 316(6): H1528-H1537
    › Primary publication · 30925081 (PubMed) · PMC6620688 (PubMed Central)
  8. Endothelial nitric oxide synthase modulates Toll-like receptor 4-mediated IL-6 production and permeability via nitric oxide-independent signaling. Stark RJ, Koch SR, Choi H, Mace EH, Dikalov SI, Sherwood ER, Lamb FS (2018) FASEB J 32(2): 945-956
    › Primary publication · 29061842 (PubMed) · PMC5888395 (PubMed Central)
  9. Comparative Transcriptome Profiles of Human Blood in Response to the Toll-like Receptor 4 Ligands Lipopolysaccharide and Monophosphoryl Lipid A. Luan L, Patil NK, Guo Y, Hernandez A, Bohannon JK, Fensterheim BA, Wang J, Xu Y, Enkhbaatar P, Stark R, Sherwood ER (2017) Sci Rep : 40050
    › Primary publication · 28053314 (PubMed) · PMC5215261 (PubMed Central)
  10. Potentiation and tolerance of toll-like receptor priming in human endothelial cells. Koch SR, Lamb FS, Hellman J, Sherwood ER, Stark RJ (2017) Transl Res : 53-67.e4
    › Primary publication · 27567430 (PubMed) · PMC5253081 (PubMed Central)
  11. Endothelial cell tolerance to lipopolysaccharide challenge is induced by monophosphoryl lipid A. Stark RJ, Choi H, Koch SR, Fensterheim BA, Lamb FS, Sherwood ER (2016) Clin Sci (Lond) 130(6): 451-61
    › Primary publication · 26669797 (PubMed) · PMC4744094 (PubMed Central)
  12. c-Jun N-terminal kinase attenuates TNFα signaling by reducing Nox1-dependent endosomal ROS production in vascular smooth muscle cells. Choi H, Dikalova A, Stark RJ, Lamb FS (2015) Free Radic Biol Med : 219-27
    › Primary publication · 26001727 (PubMed)
  13. Monophosphoryl lipid A inhibits the cytokine response of endothelial cells challenged with LPS. Stark R, Choi H, Koch S, Lamb F, Sherwood E (2015) Innate Immun 21(6): 565-74
    › Primary publication · 25540284 (PubMed) · PMC4480205 (PubMed Central)
  14. Estimating intracardiac and extracardiac shunting in the setting of complex congenital heart disease. Stark RJ, Shekerdemian LS (2013) Ann Pediatr Cardiol 6(2): 145-51
    › Primary publication · 24688231 (PubMed) · PMC3957443 (PubMed Central)
  15. Platelet-derived Toll-like receptor 4 (Tlr-4) is sufficient to promote microvascular thrombosis in endotoxemia. Stark RJ, Aghakasiri N, Rumbaut RE (2012) PLoS One 7(7): e41254
    › Primary publication · 22911769 (PubMed) · PMC3401143 (PubMed Central)
  16. Extracorporeal membrane oxygenation support of a severe metabolic crisis in a child with methylmalonic acidemia. Stark RJ, Naik-Mathuria BJ, Lam FW, Olutoye OO, Sutton VR, Shekerdemian LS (2012) ASAIO J 58(4): 438-9
    › Primary publication · 22711065 (PubMed)
  17. Modulation of CD4 Th cell differentiation by ganglioside GD1a in vitro. Shen W, Falahati R, Stark R, Leitenberg D, Ladisch S (2005) J Immunol 175(8): 4927-34
    › Primary publication · 16210594 (PubMed)