Chee Lim
Last active: 2/19/2015

Profile

The over-riding research theme in our lab is to understand the mechanisms that regulate cardiac sarcomere turnover. The cardiac sarcomere is a complex and highly ordered ensemble of contractile and regulatory proteins designed to generate force. To maintain functional sarcomeres, precise turnover of proteins is required that balances new protein synthesis and incorporation into the sarcomere with removal and degradation of worn out or damaged proteins. Titin is a giant elastic protein, spanning half a sarcomere, and forms the structural ‘backbone’ of the sarcomere; yet, the mechanisms regulating the turnover of titin are completely unknown. The goal of our lab is to use an integrative molecular biology approach to evaluate a number of key aspects in a model we have proposed for titin turnover. Our lab employs a wide range of experimental techniques in molecular biology, physiology, and bioinformatics. Experimental model systems used in our lab include neonatal and adult cardiomyocytes, isolated Langendorff-perfused hearts, mouse myocardial infarction, and genetically modified mouse models.

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Upstream open reading frame in 5'-untranslated region reduces titin mRNA translational efficiency. Cadar AG, Zhong L, Lin A, Valenzuela MO, Lim CC (2014) Biochem Biophys Res Commun 453(1): 185-91
    › Primary publication · 25264194 (PubMed) · PMC4250383 (PubMed Central)
  2. Differential activation of natriuretic peptide receptors modulates cardiomyocyte proliferation during development. Becker JR, Chatterjee S, Robinson TY, Bennett JS, Panáková D, Galindo CL, Zhong L, Shin JT, Coy SM, Kelly AE, Roden DM, Lim CC, MacRae CA (2014) Development 141(2): 335-45
    › Primary publication · 24353062 (PubMed) · PMC3879815 (PubMed Central)
  3. Poly(ε-caprolactone)-carbon nanotube composite scaffolds for enhanced cardiac differentiation of human mesenchymal stem cells. Crowder SW, Liang Y, Rath R, Park AM, Maltais S, Pintauro PN, Hofmeister W, Lim CC, Wang X, Sung HJ (2013) Nanomedicine (Lond) 8(11): 1763-76
    › Primary publication · 23530764 (PubMed) · PMC3809159 (PubMed Central)
  4. 26S proteasome regulation of Ankrd1/CARP in adult rat ventricular myocytes and human microvascular endothelial cells. Samaras SE, Chen B, Koch SR, Sawyer DB, Lim CC, Davidson JM (2012) Biochem Biophys Res Commun 425(4): 830-5
    › Primary publication · 22892129 (PubMed) · PMC3460693 (PubMed Central)
  5. The continuing evolution of the Langendorff and ejecting murine heart: new advances in cardiac phenotyping. Liao R, Podesser BK, Lim CC (2012) Am J Physiol Heart Circ Physiol 303(2): H156-67
    › Primary publication · 22636675 (PubMed) · PMC3404701 (PubMed Central)
  6. Disruption of a GATA4/Ankrd1 signaling axis in cardiomyocytes leads to sarcomere disarray: implications for anthracycline cardiomyopathy. Chen B, Zhong L, Roush SF, Pentassuglia L, Peng X, Samaras S, Davidson JM, Sawyer DB, Lim CC (2012) PLoS One 7(4): e35743
    › Primary publication · 22532871 (PubMed) · PMC3332030 (PubMed Central)
  7. Neuregulin-1β regulation of embryonic endothelial progenitor cell survival. Safa RN, Peng XY, Pentassuglia L, Lim CC, Lamparter M, Silverstein C, Walker J, Chen B, Geisberg C, Hatzopoulos AK, Sawyer DB (2011) Am J Physiol Heart Circ Physiol 300(4): H1311-9
    › Primary publication · 21239627 (PubMed) · PMC3075022 (PubMed Central)
  8. Mechanisms of anthracycline cardiac injury: can we identify strategies for cardioprotection? Sawyer DB, Peng X, Chen B, Pentassuglia L, Lim CC (2010) Prog Cardiovasc Dis 53(2): 105-13
    › Primary publication · 20728697 (PubMed) · PMC2933091 (PubMed Central)