Kevin Schey
Last active: 4/4/2019

Profile

The Schey lab is interested in both method/instrument development in proteomics analysis as well as in applications of state-of-the-art proteomics technologies in the study of development and disease. Method development/technology projects include methods for integral membrane protein analysis, tandem mass spectrometry of intact proteins (top-down proteomics), spatially-resolved proteomics analysis, and quantitative proteomics. The major area of application is human lens protein modifications and protein-protein interactions. Other areas of interest are: proteomics analysis of heart valve development, and invertebrate (shrimp and oyster) innate immunity. Integral membrane proteins play key roles in signaling, transport, and adhesion, yet they remain difficult to analyze using conventional proteomics methods. Our lab has developed methods to analyze integral membrane proteins and membrane associated proteins including their modifications. In addition, we have developed methods for membrane proteome analysis. Currently we are developing methods for spatially-resolved studies (see below), for tissue imaging of membrane proteins, and for examining the membrane protein phosphoproteome. Standard proteomics analyses typically require tryptic digestion of the protein sample followed by LC/MS/MS analysis of the peptides in order to identify and quantitate the proteins present in the sample. This bottom-up approach relies on inferring protein level information from peptide level measurements. Top-down analysis involves analysis of the intact proteins for identification but requires sophisticated technologies and software. We have developed a method for top-down proteomics analysis in a MALDI TOF-TOF platform and generated a new software application for the interpretation of resulting data. Spatially-resolved proteomics represents a critically important area of development that takes advantage of recent advances in instrumental sensitivity. The ability to examine region-specific changes in a proteome has wide applicability in both development and disease processes. Two approaches are being developed in our lab to address this issue: 1) laser capture microsdissection (LCM) coupled with shotgun proteomics analysis and 2) MALDI tissue imaging. We have combined our membrane protein analysis protocols with LCM capture to examine region-specific changes in the ocular lens membrane proteome. Also, we have developed new sample preparation methods to allow MALDI imaging of membrane proteins. The MALDI imaging method is being applied to lens aging, chick and mouse heart development, shrimp viral infection, brain changes in addiction, and lung infection. Our long-standing project on ocular lens membrane proteins is focused on how these proteins change as lens cells differentiate and as they age. Since the proteins in the lens core are as old as the individual, the lens serves as a model tissue to study protein aging. Age- and cataract-specific modifications have been identified and regional changes in the membrane proteome are being examined. Functional consequences of lens protein modification are also being examined with confocal microscopic, molecular biology, and spectroscopic methods.

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Multistate design of influenza antibodies improves affinity and breadth against seasonal viruses. Sevy AM, Wu NC, Gilchuk IM, Parrish EH, Burger S, Yousif D, Nagel MBM, Schey KL, Wilson IA, Crowe JE, Meiler J (2019) Proc Natl Acad Sci U S A 116(5): 1597-1602
    › Primary publication · 30642961 (PubMed) · PMC6358683 (PubMed Central)
  2. Human mAbs to Staphylococcus aureus IsdA Provide Protection Through Both Heme-Blocking and Fc-Mediated Mechanisms. Bennett MR, Bombardi RG, Kose N, Parrish EH, Nagel MB, Petit RA, Read TD, Schey KL, Thomsen IP, Skaar EP, Crowe JE (2019) J Infect Dis 219(8): 1264-1273
    › Primary publication · 30496483 (PubMed) · PMC6452300 (PubMed Central)
  3. Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20. Wang Z, Schey KL (2018) Invest Ophthalmol Vis Sci 59(13): 5648-5658
    › Primary publication · 30489624 (PubMed) · PMC6266727 (PubMed Central)
  4. Carcinogenic Strains Selectively Dysregulate the Gastric Proteome, Which May Be Associated with Stomach Cancer Progression. Noto JM, Rose KL, Hachey AJ, Delgado AG, Romero-Gallo J, Wroblewski LE, Schneider BG, Shah SC, Cover TL, Wilson KT, Israel DA, Roa JC, Schey KL, Zavros Y, Piazuelo MB, Peek RM (2019) Mol Cell Proteomics 18(2): 352-371
    › Primary publication · 30455363 (PubMed) · PMC6356085 (PubMed Central)
  5. Spontaneous cross-linking of proteins at aspartate and asparagine residues is mediated via a succinimide intermediate. Friedrich MG, Wang Z, Schey KL, Truscott RJW (2018) Biochem J 475(20): 3189-3200
    › Primary publication · 30181147 (PubMed)
  6. Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury. Noble KV, Reyzer ML, Barth JL, McDonald H, Tuck M, Schey KL, Krug EL, Lang H (2018) Front Mol Neurosci : 243
    › Primary publication · 30065626 (PubMed) · PMC6056684 (PubMed Central)
  7. Quantification of thioether-linked glutathione modifications in human lens proteins. Wang Z, Schey KL (2018) Exp Eye Res : 83-89
    › Primary publication · 29879394 (PubMed) · PMC6167147 (PubMed Central)
  8. Yale School of Public Health Symposium on tissue imaging mass spectrometry: illuminating phenotypic heterogeneity and drug disposition at the molecular level. Charkoftaki G, Rattray NJW, Andrén PE, Caprioli RM, Castellino S, Duncan MW, Goodwin RJA, Schey KL, Shahidi-Latham SK, Veselkov KA, Johnson CH, Vasiliou V (2018) Hum Genomics 12(1): 10
    › Primary publication · 29482659 (PubMed) · PMC5828306 (PubMed Central)
  9. A specific phosphorylation regulates the protective role of αA-crystallin in diabetes. Ruebsam A, Dulle JE, Myers AM, Sakrikar D, Green KM, Khan NW, Schey K, Fort PE (2018) JCI Insight 3(4)
    › Primary publication · 29467334 (PubMed) · PMC5916248 (PubMed Central)
  10. Optic Nerve Regeneration After Crush Remodels the Injury Site: Molecular Insights From Imaging Mass Spectrometry. Stark DT, Anderson DMG, Kwong JMK, Patterson NH, Schey KL, Caprioli RM, Caprioli J (2018) Invest Ophthalmol Vis Sci 59(1): 212-222
    › Primary publication · 29340649 (PubMed) · PMC5770179 (PubMed Central)
  11. DehydroalanylGly, a new post translational modification resulting from the breakdown of glutathione. Friedrich MG, Wang Z, Schey KL, Truscott RJW (2018) Biochim Biophys Acta Gen Subj 1862(4): 907-913
    › Primary publication · 29309825 (PubMed)
  12. The Role of Aquaporins in Ocular Lens Homeostasis. Schey KL, Petrova RS, Gletten RB, Donaldson PJ (2017) Int J Mol Sci 18(12)
    › Primary publication · 29231874 (PubMed) · PMC5751294 (PubMed Central)
  13. Bis(monoacylglycero)phosphate lipids in the retinal pigment epithelium implicate lysosomal/endosomal dysfunction in a model of Stargardt disease and human retinas. Anderson DMG, Ablonczy Z, Koutalos Y, Hanneken AM, Spraggins JM, Calcutt MW, Crouch RK, Caprioli RM, Schey KL (2017) Sci Rep 7(1): 17352
    › Primary publication · 29229934 (PubMed) · PMC5725462 (PubMed Central)
  14. Loss of αB-crystallin function in zebrafish reveals critical roles in the development of the lens and stress resistance of the heart. Mishra S, Wu SY, Fuller AW, Wang Z, Rose KL, Schey KL, Mchaourab HS (2018) J Biol Chem 293(2): 740-753
    › Primary publication · 29162721 (PubMed) · PMC5767876 (PubMed Central)
  15. Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells. Petrova RS, Webb KF, Vaghefi E, Walker K, Schey KL, Donaldson PJ (2018) Am J Physiol Cell Physiol 314(2): C191-C201
    › Primary publication · 29118028 (PubMed) · PMC5866439 (PubMed Central)
  16. MALDI (matrix assisted laser desorption ionization) Imaging Mass Spectrometry (IMS) of skin: Aspects of sample preparation. de Macedo CS, Anderson DM, Schey KL (2017) Talanta : 325-335
    › Primary publication · 28738588 (PubMed)
  17. Hotspots of age-related protein degradation: the importance of neighboring residues for the formation of non-disulfide crosslinks derived from cysteine. Friedrich MG, Wang Z, Oakley AJ, Schey KL, Truscott RJW (2017) Biochem J 474(14): 2475-2487
    › Primary publication · 28592682 (PubMed) · PMC5685506 (PubMed Central)
  18. Imaging MS of Rodent Ocular Tissues and the Optic Nerve. Anderson DMG, Lambert W, Calkins DJ, Ablonczy Z, Crouch RK, Caprioli RM, Schey KL (2017) Methods Mol Biol : 15-27
    › Primary publication · 28523496 (PubMed)
  19. Identification of a direct Aquaporin-0 binding site in the lens-specific cytoskeletal protein filensin. Wang Z, Schey KL (2017) Exp Eye Res : 23-29
    › Primary publication · 28259670 (PubMed) · PMC5471609 (PubMed Central)
  20. Corneal haze phenotype in Aldh3a1-null mice: In vivo confocal microscopy and tissue imaging mass spectrometry. Chen Y, Jester JV, Anderson DM, Marchitti SA, Schey KL, Thompson DC, Vasiliou V (2017) Chem Biol Interact : 9-14
    › Primary publication · 28038895 (PubMed)
  21. Spatial distributions of glutathione and its endogenous conjugates in normal bovine lens and a model of lens aging. Nye-Wood MG, Spraggins JM, Caprioli RM, Schey KL, Donaldson PJ, Grey AC (2017) Exp Eye Res : 70-78
    › Primary publication · 27838309 (PubMed) · PMC5359059 (PubMed Central)
  22. Identification and Functional Assessment of Age-Dependent Truncations to Cx46 and Cx50 in the Human Lens. Slavi N, Wang Z, Harvey L, Schey KL, Srinivas M (2016) Invest Ophthalmol Vis Sci 57(13): 5714-5722
    › Primary publication · 27787559 (PubMed) · PMC5089213 (PubMed Central)
  23. Expression of Cataract-linked γ-Crystallin Variants in Zebrafish Reveals a Proteostasis Network That Senses Protein Stability. Wu SY, Zou P, Fuller AW, Mishra S, Wang Z, Schey KL, Mchaourab HS (2016) J Biol Chem 291(49): 25387-25397
    › Primary publication · 27770023 (PubMed) · PMC5207241 (PubMed Central)
  24. Spatially Directed Proteomics of the Human Lens Outer Cortex Reveals an Intermediate Filament Switch Associated With the Remodeling Zone. Wenke JL, McDonald WH, Schey KL (2016) Invest Ophthalmol Vis Sci 57(10): 4108-14
    › Primary publication · 27537260 (PubMed) · PMC4991037 (PubMed Central)
  25. Old Proteins in Man: A Field in its Infancy. Truscott RJW, Schey KL, Friedrich MG (2016) Trends Biochem Sci 41(8): 654-664
    › Primary publication · 27426990 (PubMed) · PMC5500981 (PubMed Central)
  26. Spatial distributions of phosphorylated membrane proteins aquaporin 0 and MP20 across young and aged human lenses. Gutierrez DB, Garland DL, Schwacke JH, Hachey DL, Schey KL (2016) Exp Eye Res : 59-65
    › Primary publication · 27339748 (PubMed) · PMC5791148 (PubMed Central)
  27. Back cover: Imaging mass spectrometry of the visual system: Advancing the molecular understanding of retina degenerations. Bowrey HE, Anderson DM, Pallitto P, Gutierrez DB, Fan J, Crouch RK, Schey KL, Ablonczy Z (2016) Proteomics Clin Appl 10(4): NA
    › Primary publication · 27061327 (PubMed)
  28. MALDI imaging mass spectrometry of Pacific White Shrimp L. vannamei and identification of abdominal muscle proteins. Schey KL, Hachey AJ, Rose KL, Grey AC (2016) Proteomics 16(11-12): 1767-74
    › Primary publication · 26990122 (PubMed)
  29. 3-D imaging mass spectrometry of protein distributions in mouse Neurofibromatosis 1 (NF1)-associated optic glioma. Anderson DM, Van de Plas R, Rose KL, Hill S, Schey KL, Solga AC, Gutmann DH, Caprioli RM (2016) J Proteomics : 77-84
    › Primary publication · 26883872 (PubMed) · PMC4983271 (PubMed Central)
  30. Proteomic Analysis of Lipid Raft-Like Detergent-Resistant Membranes of Lens Fiber Cells. Wang Z, Schey KL (2015) Invest Ophthalmol Vis Sci 56(13): 8349-60
    › Primary publication · 26747763 (PubMed) · PMC4699431 (PubMed Central)
  31. MALDI imaging reveals lipid changes in the skin of leprosy patients before and after multidrug therapy (MDT). de Macedo CS, Anderson DM, Pascarelli BM, Spraggins JM, Sarno EN, Schey KL, Pessolani MC (2015) J Mass Spectrom 50(12): 1374-85
    › Primary publication · 26634971 (PubMed)
  32. Imaging mass spectrometry of the visual system: Advancing the molecular understanding of retina degenerations. Bowrey HE, Anderson DM, Pallitto P, Gutierrez DB, Fan J, Crouch RK, Schey KL, Ablonczy Z (2016) Proteomics Clin Appl 10(4): 391-402
    › Primary publication · 26586164 (PubMed)
  33. MALDI Imaging Mass Spectrometry Spatially Maps Age-Related Deamidation and Truncation of Human Lens Aquaporin-0. Wenke JL, Rose KL, Spraggins JM, Schey KL (2015) Invest Ophthalmol Vis Sci 56(12): 7398-405
    › Primary publication · 26574799 (PubMed) · PMC4675204 (PubMed Central)
  34. Proteomic Analysis of Macular Fluid Associated With Advanced Glaucomatous Excavation. Patel S, Ling J, Kim SJ, Schey KL, Rose K, Kuchtey RW (2016) JAMA Ophthalmol 134(1): 108-10
    › Primary publication · 26540576 (PubMed) · PMC5523109 (PubMed Central)
  35. Determination of N-retinylidene-N-retinylethanolamine (A2E) levels in central and peripheral areas of human retinal pigment epithelium. Adler L, Boyer NP, Anderson DM, Spraggins JM, Schey KL, Hanneken A, Ablonczy Z, Crouch RK, Koutalos Y (2015) Photochem Photobiol Sci 14(11): 1983-90
    › Primary publication · 26323192 (PubMed) · PMC4626405 (PubMed Central)
  36. A2E and Lipofuscin. Crouch RK, Koutalos Y, Kono M, Schey K, Ablonczy Z (2015) Prog Mol Biol Transl Sci : 449-63
    › Primary publication · 26310170 (PubMed)
  37. Activation of the Endogenous Renin-Angiotensin-Aldosterone System or Aldosterone Administration Increases Urinary Exosomal Sodium Channel Excretion. Qi Y, Wang X, Rose KL, MacDonald WH, Zhang B, Schey KL, Luther JM (2016) J Am Soc Nephrol 27(2): 646-56
    › Primary publication · 26113616 (PubMed) · PMC4731116 (PubMed Central)
  38. High spatial resolution imaging mass spectrometry of human optic nerve lipids and proteins. Anderson DM, Spraggins JM, Rose KL, Schey KL (2015) J Am Soc Mass Spectrom 26(6): 940-7
    › Primary publication · 25893273 (PubMed) · PMC5650057 (PubMed Central)
  39. Proteomics characterization of exosome cargo. Schey KL, Luther JM, Rose KL (2015) Methods : 75-82
    › Primary publication · 25837312 (PubMed) · PMC4591097 (PubMed Central)
  40. A method to prevent protein delocalization in imaging mass spectrometry of non-adherent tissues: application to small vertebrate lens imaging. Anderson DM, Floyd KA, Barnes S, Clark JM, Clark JI, Mchaourab H, Schey KL (2015) Anal Bioanal Chem 407(8): 2311-20
    › Primary publication · 25665708 (PubMed) · PMC4379205 (PubMed Central)
  41. Role of αA-crystallin-derived αA66-80 peptide in guinea pig lens crystallin aggregation and insolubilization. Raju M, Mooney BP, Thakkar KM, Giblin FJ, Schey KL, Sharma KK (2015) Exp Eye Res : 151-60
    › Primary publication · 25639202 (PubMed) · PMC4352376 (PubMed Central)
  42. Spatial distributions of AQP5 and AQP0 in embryonic and postnatal mouse lens development. Petrova RS, Schey KL, Donaldson PJ, Grey AC (2015) Exp Eye Res : 124-35
    › Primary publication · 25595964 (PubMed) · PMC4352382 (PubMed Central)
  43. Synergistic effect of pro-inflammatory TNFα and IL-17 in periostin mediated collagen deposition: potential role in liver fibrosis. Amara S, Lopez K, Banan B, Brown SK, Whalen M, Myles E, Ivy MT, Johnson T, Schey KL, Tiriveedhi V (2015) Mol Immunol 64(1): 26-35
    › Primary publication · 25467797 (PubMed) · PMC4282823 (PubMed Central)
  44. High resolution MALDI imaging mass spectrometry of retinal tissue lipids. Anderson DM, Ablonczy Z, Koutalos Y, Spraggins J, Crouch RK, Caprioli RM, Schey KL (2014) J Am Soc Mass Spectrom 25(8): 1394-403
    › Primary publication · 24819461 (PubMed) · PMC4180438 (PubMed Central)
  45. Imbalanced expression of Vcan mRNA splice form proteins alters heart morphology and cellular protein profiles. Burns TA, Dours-Zimmermann MT, Zimmermann DR, Krug EL, Comte-Walters S, Reyes L, Davis MA, Schey KL, Schwacke JH, Kern CB, Mjaatvedt CH (2014) PLoS One 9(2): e89133
    › Primary publication · 24586547 (PubMed) · PMC3930639 (PubMed Central)
  46. Differential localization of G protein βγ subunits. Betke KM, Rose KL, Friedman DB, Baucum AJ, Hyde K, Schey KL, Hamm HE (2014) Biochemistry 53(14): 2329-43
    › Primary publication · 24568373 (PubMed) · PMC4004276 (PubMed Central)
  47. Activation of EGFR and ERBB2 by Helicobacter pylori results in survival of gastric epithelial cells with DNA damage. Chaturvedi R, Asim M, Piazuelo MB, Yan F, Barry DP, Sierra JC, Delgado AG, Hill S, Casero RA, Bravo LE, Dominguez RL, Correa P, Polk DB, Washington MK, Rose KL, Schey KL, Morgan DR, Peek RM, Wilson KT (2014) Gastroenterology 146(7): 1739-51.e14
    › Primary publication · 24530706 (PubMed) · PMC4035375 (PubMed Central)
  48. The utilization of fluorescence to identify the components of lipofuscin by imaging mass spectrometry. Ablonczy Z, Smith N, Anderson DM, Grey AC, Spraggins J, Koutalos Y, Schey KL, Crouch RK (2014) Proteomics 14(7-8): 936-44
    › Primary publication · 24453194 (PubMed) · PMC4017854 (PubMed Central)
  49. Tyrosine/cysteine cluster sensitizing human γD-crystallin to ultraviolet radiation-induced photoaggregation in vitro. Schafheimer N, Wang Z, Schey K, King J (2014) Biochemistry 53(6): 979-90
    › Primary publication · 24410332 (PubMed) · PMC3954642 (PubMed Central)
  50. Hands-on workshops as an effective means of learning advanced technologies including genomics, proteomics and bioinformatics. Reisdorph N, Stearman R, Kechris K, Phang TL, Reisdorph R, Prenni J, Erle DJ, Coldren C, Schey K, Nesvizhskii A, Geraci M (2013) Genomics Proteomics Bioinformatics 11(6): 368-77
    › Primary publication · 24316330 (PubMed) · PMC4049090 (PubMed Central)
  51. Aquaporins in the eye: expression, function, and roles in ocular disease. Schey KL, Wang Z, L Wenke J, Qi Y (2014) Biochim Biophys Acta 1840(5): 1513-23
    › Primary publication · 24184915 (PubMed) · PMC4572841 (PubMed Central)
  52. Human protein aging: modification and crosslinking through dehydroalanine and dehydrobutyrine intermediates. Wang Z, Lyons B, Truscott RJ, Schey KL (2014) Aging Cell 13(2): 226-34
    › Primary publication · 24134651 (PubMed) · PMC4114717 (PubMed Central)
  53. Similar molecules spatially correlate with lipofuscin and N-retinylidene-N-retinylethanolamine in the mouse but not in the human retinal pigment epithelium. Ablonczy Z, Higbee D, Grey AC, Koutalos Y, Schey KL, Crouch RK (2013) Arch Biochem Biophys 539(2): 196-202
    › Primary publication · 23969078 (PubMed) · PMC3818512 (PubMed Central)
  54. Lack of correlation between the spatial distribution of A2E and lipofuscin fluorescence in the human retinal pigment epithelium. Ablonczy Z, Higbee D, Anderson DM, Dahrouj M, Grey AC, Gutierrez D, Koutalos Y, Schey KL, Hanneken A, Crouch RK (2013) Invest Ophthalmol Vis Sci 54(8): 5535-42
    › Primary publication · 23847313 (PubMed) · PMC3747789 (PubMed Central)
  55. MALDI imaging and in situ identification of integral membrane proteins from rat brain tissue sections. Nicklay JJ, Harris GA, Schey KL, Caprioli RM (2013) Anal Chem 85(15): 7191-6
    › Primary publication · 23829295 (PubMed) · PMC3782084 (PubMed Central)
  56. L-type calcium channels play a critical role in maintaining lens transparency by regulating phosphorylation of aquaporin-0 and myosin light chain and expression of connexins. Maddala R, Nagendran T, de Ridder GG, Schey KL, Rao PV (2013) PLoS One 8(5): e64676
    › Primary publication · 23734214 (PubMed) · PMC3667166 (PubMed Central)
  57. Spatially-directed protein identification from tissue sections by top-down LC-MS/MS with electron transfer dissociation. Schey KL, Anderson DM, Rose KL (2013) Anal Chem 85(14): 6767-74
    › Primary publication · 23718750 (PubMed) · PMC3749783 (PubMed Central)
  58. High-resolution matrix-assisted laser desorption ionization-imaging mass spectrometry of lipids in rodent optic nerve tissue. Anderson DM, Mills D, Spraggins J, Lambert WS, Calkins DJ, Schey KL (2013) Mol Vis : 581-92
    › Primary publication · 23559852 (PubMed) · PMC3611942 (PubMed Central)
  59. Mass spectrometry of membrane proteins: a focus on aquaporins. Schey KL, Grey AC, Nicklay JJ (2013) Biochemistry 52(22): 3807-17
    › Primary publication · 23394619 (PubMed) · PMC4570568 (PubMed Central)
  60. Proteomics and phosphoproteomics analysis of human lens fiber cell membranes. Wang Z, Han J, David LL, Schey KL (2013) Invest Ophthalmol Vis Sci 54(2): 1135-43
    › Primary publication · 23349431 (PubMed) · PMC3567755 (PubMed Central)
  61. Verification and spatial localization of aquaporin-5 in the ocular lens. Grey AC, Walker KL, Petrova RS, Han J, Wilmarth PA, David LL, Donaldson PJ, Schey KL (2013) Exp Eye Res : 94-102
    › Primary publication · 23313152 (PubMed) · PMC3583522 (PubMed Central)
  62. Identification of a novel metal binding protein, segon, in plasma of the eastern oyster, Crassostrea virginica. Xue Q, Gauthier J, Schey K, Li Y, Cooper R, Anderson R, La Peyre J (2012) Comp Biochem Physiol B Biochem Mol Biol 163(1): 74-85
    › Primary publication · 22580268 (PubMed)
  63. Molecule-specific imaging and quantitation of A2E in the RPE. Ablonczy Z, Gutierrez DB, Grey AC, Schey KL, Crouch RK (2012) Adv Exp Med Biol : 75-81
    › Primary publication · 22183318 (PubMed) · PMC3817715 (PubMed Central)
  64. Proteomic analysis of urine exosomes by multidimensional protein identification technology (MudPIT). Wang Z, Hill S, Luther JM, Hachey DL, Schey KL (2012) Proteomics 12(2): 329-38
    › Primary publication · 22106071 (PubMed) · PMC3517144 (PubMed Central)
  65. Spatial analysis of human lens aquaporin-0 post-translational modifications by MALDI mass spectrometry tissue profiling. Gutierrez DB, Garland D, Schey KL (2011) Exp Eye Res 93(6): 912-20
    › Primary publication · 22036630 (PubMed) · PMC3267704 (PubMed Central)
  66. Cryptococcus neoformans modulates extracellular killing by neutrophils. Qureshi A, Grey A, Rose KL, Schey KL, Del Poeta M (2011) Front Microbiol : 193
    › Primary publication · 21960987 (PubMed) · PMC3177079 (PubMed Central)
  67. Structural basis for the Trembler-J phenotype of Charcot-Marie-Tooth disease. Sakakura M, Hadziselimovic A, Wang Z, Schey KL, Sanders CR (2011) Structure 19(8): 1160-9
    › Primary publication · 21827951 (PubMed) · PMC3154623 (PubMed Central)
  68. Aquaporin-0 interacts with the FERM domain of ezrin/radixin/moesin proteins in the ocular lens. Wang Z, Schey KL (2011) Invest Ophthalmol Vis Sci 52(8): 5079-87
    › Primary publication · 21642618 (PubMed) · PMC3176042 (PubMed Central)
  69. AKAP signaling in reinstated cocaine seeking revealed by iTRAQ proteomic analysis. Reissner KJ, Uys JD, Schwacke JH, Comte-Walters S, Rutherford-Bethard JL, Dunn TE, Blumer JB, Schey KL, Kalivas PW (2011) J Neurosci 31(15): 5648-58
    › Primary publication · 21490206 (PubMed) · PMC3595532 (PubMed Central)
  70. Protein profile of exosomes from trabecular meshwork cells. Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL (2011) J Proteomics 74(6): 796-804
    › Primary publication · 21362503 (PubMed) · PMC3085584 (PubMed Central)
  71. Spatial localization of A2E in the retinal pigment epithelium. Grey AC, Crouch RK, Koutalos Y, Schey KL, Ablonczy Z (2011) Invest Ophthalmol Vis Sci 52(7): 3926-33
    › Primary publication · 21357388 (PubMed) · PMC3175942 (PubMed Central)
  72. Role of sphingomyelin synthase in controlling the antimicrobial activity of neutrophils against Cryptococcus neoformans. Qureshi A, Subathra M, Grey A, Schey K, Del Poeta M, Luberto C (2010) PLoS One 5(12): e15587
    › Primary publication · 21203393 (PubMed) · PMC3011003 (PubMed Central)
  73. Tight binding of proteins to membranes from older human cells. Truscott RJ, Comte-Walters S, Ablonczy Z, Schwacke JH, Berry Y, Korlimbinis A, Friedrich MG, Schey KL (2011) Age (Dordr) 33(4): 543-54
    › Primary publication · 21181282 (PubMed) · PMC3220407 (PubMed Central)
  74. Novel fatty acid acylation of lens integral membrane protein aquaporin-0. Schey KL, Gutierrez DB, Wang Z, Wei J, Grey AC (2010) Biochemistry 49(45): 9858-65
    › Primary publication · 20942504 (PubMed) · PMC3831505 (PubMed Central)
  75. Mass spectrometry provides accurate and sensitive quantitation of A2E. Gutierrez DB, Blakeley L, Goletz PW, Schey KL, Hanneken A, Koutalos Y, Crouch RK, Ablonczy Z (2010) Photochem Photobiol Sci 9(11): 1513-9
    › Primary publication · 20931136 (PubMed) · PMC3013054 (PubMed Central)
  76. A new lysozyme from the eastern oyster, Crassostrea virginica, and a possible evolutionary pathway for i-type lysozymes in bivalves from host defense to digestion. Xue Q, Hellberg ME, Schey KL, Itoh N, Eytan RI, Cooper RK, La Peyre JF (2010) BMC Evol Biol : 213
    › Primary publication · 20633278 (PubMed) · PMC3020801 (PubMed Central)
  77. Characterization of the major plasma protein of the eastern oyster, Crassostrea virginica, and a proposed role in host defense. Itoh N, Xue QG, Schey KL, Li Y, Cooper RK, La Peyre JF (2011) Comp Biochem Physiol B Biochem Mol Biol 158(1): 9-22
    › Primary publication · 20601063 (PubMed)
  78. Tissue localization and solubilities of αA-crystallin and its numerous C-terminal truncation products in pre- and postcataractous ICR/f rat lenses. Stella DR, Floyd KA, Grey AC, Renfrow MB, Schey KL, Barnes S (2010) Invest Ophthalmol Vis Sci 51(10): 5153-61
    › Primary publication · 20435586 (PubMed) · PMC3066599 (PubMed Central)
  79. Molecular morphology of the chick heart visualized by MALDI imaging mass spectrometry. Grey AC, Gelasco AK, Section J, Moreno-Rodriguez RA, Krug EL, Schey KL (2010) Anat Rec (Hoboken) 293(5): 821-8
    › Primary publication · 20186963 (PubMed) · PMC3089908 (PubMed Central)
  80. Matrix-assisted laser desorption/ionization tissue profiling of secretoneurin in the nucleus accumbens shell from cocaine-sensitized rats. Uys JD, Grey AC, Wiggins A, Schwacke JH, Schey KL, Kalivas PW (2010) J Mass Spectrom 45(1): 97-103
    › Primary publication · 19918966 (PubMed) · PMC4153729 (PubMed Central)
  81. Posttranslational modifications of the bovine lens beaded filament proteins filensin and CP49. Wang Z, Obidike JE, Schey KL (2010) Invest Ophthalmol Vis Sci 51(3): 1565-74
    › Primary publication · 19875662 (PubMed) · PMC2868428 (PubMed Central)
  82. iQuantitator: a tool for protein expression inference using iTRAQ. Schwacke JH, Hill EG, Krug EL, Comte-Walters S, Schey KL (2009) BMC Bioinformatics : 342
    › Primary publication · 19835628 (PubMed) · PMC2770557 (PubMed Central)
  83. Phosphorylation and truncation sites of bovine lens connexin 46 and connexin 50. Wang Z, Schey KL (2009) Exp Eye Res 89(6): 898-904
    › Primary publication · 19646399 (PubMed) · PMC2783236 (PubMed Central)
  84. Quantification of protein expression changes in the aging left ventricle of Rattus norvegicus. Grant JE, Bradshaw AD, Schwacke JH, Baicu CF, Zile MR, Schey KL (2009) J Proteome Res 8(9): 4252-63
    › Primary publication · 19603826 (PubMed) · PMC2752960 (PubMed Central)
  85. Evidence indicating the existence of a novel family of serine protease inhibitors that may be involved in marine invertebrate immunity. Xue Q, Itoh N, Schey KL, Cooper RK, La Peyre JF (2009) Fish Shellfish Immunol 27(2): 250-9
    › Primary publication · 19464375 (PubMed)
  86. Age-related changes in the spatial distribution of human lens alpha-crystallin products by MALDI imaging mass spectrometry. Grey AC, Schey KL (2009) Invest Ophthalmol Vis Sci 50(9): 4319-29
    › Primary publication · 19387068 (PubMed) · PMC2760347 (PubMed Central)
  87. Confocal microscopy reveals zones of membrane remodeling in the outer cortex of the human lens. Lim JC, Walker KL, Sherwin T, Schey KL, Donaldson PJ (2009) Invest Ophthalmol Vis Sci 50(9): 4304-10
    › Primary publication · 19357350 (PubMed) · PMC4572844 (PubMed Central)
  88. MALDI imaging mass spectrometry of integral membrane proteins from ocular lens and retinal tissue. Grey AC, Chaurand P, Caprioli RM, Schey KL (2009) J Proteome Res 8(7): 3278-83
    › Primary publication · 19326924 (PubMed) · PMC2715141 (PubMed Central)
  89. Differentiation-dependent modification and subcellular distribution of aquaporin-0 suggests multiple functional roles in the rat lens. Grey AC, Li L, Jacobs MD, Schey KL, Donaldson PJ (2009) Differentiation 77(1): 70-83
    › Primary publication · 19281766 (PubMed) · PMC2696237 (PubMed Central)
  90. Protein aging: truncation of aquaporin 0 in human lens regions is a continuous age-dependent process. Korlimbinis A, Berry Y, Thibault D, Schey KL, Truscott RJ (2009) Exp Eye Res 88(5): 966-73
    › Primary publication · 19135052 (PubMed) · PMC2713177 (PubMed Central)
  91. Contributions of functional genomics and proteomics to the study of immune responses in the Pacific white leg shrimp Litopenaeus vannamei. Robalino J, Carnegie RB, O'Leary N, Ouvry-Patat SA, de la Vega E, Prior S, Gross PS, Browdy CL, Chapman RW, Schey KL, Warr G (2009) Vet Immunol Immunopathol 128(1-3): 110-8
    › Primary publication · 19070907 (PubMed)
  92. A statistical model for iTRAQ data analysis. Hill EG, Schwacke JH, Comte-Walters S, Slate EH, Oberg AL, Eckel-Passow JE, Therneau TM, Schey KL (2008) J Proteome Res 7(8): 3091-101
    › Primary publication · 18578521 (PubMed) · PMC2722948 (PubMed Central)
  93. Spatial differences in an integral membrane proteome detected in laser capture microdissected samples. Wang Z, Han J, Schey KL (2008) J Proteome Res 7(7): 2696-702
    › Primary publication · 18489132 (PubMed) · PMC2740381 (PubMed Central)
  94. MALDI tissue profiling of integral membrane proteins from ocular tissues. Thibault DB, Gillam CJ, Grey AC, Han J, Schey KL (2008) J Am Soc Mass Spectrom 19(6): 814-22
    › Primary publication · 18396059 (PubMed) · PMC2430993 (PubMed Central)
  95. Distribution of bovine and rabbit lens alpha-crystallin products by MALDI imaging mass spectrometry. Grey AC, Schey KL (2008) Mol Vis : 171-9
    › Primary publication · 18334935 (PubMed) · PMC2254960 (PubMed Central)
  96. Aquaporin 0-calmodulin interaction and the effect of aquaporin 0 phosphorylation. Rose KM, Wang Z, Magrath GN, Hazard ES, Hildebrandt JD, Schey KL (2008) Biochemistry 47(1): 339-47
    › Primary publication · 18081321 (PubMed)
  97. Fragmentation of multiply-charged intact protein ions using MALDI TOF-TOF mass spectrometry. Liu Z, Schey KL (2008) J Am Soc Mass Spectrom 19(2): 231-8
    › Primary publication · 17693096 (PubMed) · PMC2288703 (PubMed Central)
  98. Characterization of antimicrobial histone sequences and posttranslational modifications by mass spectrometry. Ouvry-Patat SA, Schey KL (2007) J Mass Spectrom 42(5): 664-74
    › Primary publication · 17405180 (PubMed)
  99. A new lysozyme from the eastern oyster (Crassostrea virginica) indicates adaptive evolution of i-type lysozymes. Xue QG, Itoh N, Schey KL, Li YL, Cooper RK, La Peyre JF (2007) Cell Mol Life Sci 64(1): 82-95
    › Primary publication · 17160350 (PubMed)
  100. A novel slow-tight binding serine protease inhibitor from eastern oyster (Crassostrea virginica) plasma inhibits perkinsin, the major extracellular protease of the oyster protozoan parasite Perkinsus marinus. Xue QG, Waldrop GL, Schey KL, Itoh N, Ogawa M, Cooper RK, Losso JN, La Peyre JF (2006) Comp Biochem Physiol B Biochem Mol Biol 145(1): 16-26
    › Primary publication · 16872855 (PubMed)
  101. MALDI tissue imaging of ocular lens alpha-crystallin. Han J, Schey KL (2006) Invest Ophthalmol Vis Sci 47(7): 2990-6
    › Primary publication · 16799044 (PubMed)
  102. ADP-ribosylation of cyclophilin A by Pseudomonas aeruginosa exoenzyme S. DiNovo AA, Schey KL, Vachon WS, McGuffie EM, Olson JC, Vincent TS (2006) Biochemistry 45(14): 4664-73
    › Primary publication · 16584201 (PubMed)
  103. The C terminus of lens aquaporin 0 interacts with the cytoskeletal proteins filensin and CP49. Lindsey Rose KM, Gourdie RG, Prescott AR, Quinlan RA, Crouch RK, Schey KL (2006) Invest Ophthalmol Vis Sci 47(4): 1562-70
    › Primary publication · 16565393 (PubMed)
  104. A multidimensional proteomic approach to identify hypertrophy-associated proteins. Lindsey ML, Goshorn DK, Comte-Walters S, Hendrick JW, Hapke E, Zile MR, Schey K (2006) Proteomics 6(7): 2225-35
    › Primary publication · 16493702 (PubMed)
  105. Circular dichroism and cross-linking studies of bacteriorhodopsin mutants. Karnaukhova E, Schey KL, Crouch RK (2006) Amino Acids 30(1): 17-23
    › Primary publication · 16477391 (PubMed)
  106. Proteomic analysis of bovine brain G protein gamma subunit processing heterogeneity. Cook LA, Schey KL, Wilcox MD, Dingus J, Ettling R, Nelson T, Knapp DR, Hildebrandt JD (2006) Mol Cell Proteomics 5(4): 671-85
    › Primary publication · 16332732 (PubMed)
  107. Optimization of a MALDI TOF-TOF mass spectrometer for intact protein analysis. Liu Z, Schey KL (2005) J Am Soc Mass Spectrom 16(4): 482-90
    › Primary publication · 15792717 (PubMed)
  108. Phosphorylation and glycosylation of bovine lens MP20. Ervin LA, Ball LE, Crouch RK, Schey KL (2005) Invest Ophthalmol Vis Sci 46(2): 627-35
    › Primary publication · 15671292 (PubMed)
  109. Antimicrobial activity of histones from hemocytes of the Pacific white shrimp. Patat SA, Carnegie RB, Kingsbury C, Gross PS, Chapman R, Schey KL (2004) Eur J Biochem 271(23-24): 4825-33
    › Primary publication · 15606770 (PubMed)
  110. Purification and characterization of lysozyme from plasma of the eastern oyster (Crassostrea virginica). Xue QG, Schey KL, Volety AK, Chu FL, La Peyre JF (2004) Comp Biochem Physiol B Biochem Mol Biol 139(1): 11-25
    › Primary publication · 15364284 (PubMed)
  111. Proteolysis and mass spectrometric analysis of an integral membrane: aquaporin 0. Han J, Schey KL (2004) J Proteome Res 3(4): 807-12
    › Primary publication · 15359735 (PubMed)
  112. Post-translational modifications of aquaporin 0 (AQP0) in the normal human lens: spatial and temporal occurrence. Ball LE, Garland DL, Crouch RK, Schey KL (2004) Biochemistry 43(30): 9856-65
    › Primary publication · 15274640 (PubMed)
  113. Diversity of metallothioneins in the American oyster, Crassostrea virginica, revealed by transcriptomic and proteomic approaches. Jenny MJ, Ringwood AH, Schey K, Warr GW, Chapman RW (2004) Eur J Biochem 271(9): 1702-12
    › Primary publication · 15096209 (PubMed)
  114. Sequence and peptide map of guinea pig aquaporin 0. Han J, Little M, David LL, Giblin FJ, Schey KL (2004) Mol Vis : 215-22
    › Primary publication · 15064681 (PubMed)
  115. Water permeability of C-terminally truncated aquaporin 0 (AQP0 1-243) observed in the aging human lens. Ball LE, Little M, Nowak MW, Garland DL, Crouch RK, Schey KL (2003) Invest Ophthalmol Vis Sci 44(11): 4820-8
    › Primary publication · 14578404 (PubMed)
  116. Gamma 2 subunit of G protein heterotrimer is an N-end rule ubiquitylation substrate. Hamilton MH, Cook LA, McRackan TR, Schey KL, Hildebrandt JD (2003) Proc Natl Acad Sci U S A 100(9): 5081-6
    › Primary publication · 12700354 (PubMed) · PMC154301 (PubMed Central)
  117. High glucose and insulin promote O-GlcNAc modification of proteins, including alpha-tubulin. Walgren JL, Vincent TS, Schey KL, Buse MG (2003) Am J Physiol Endocrinol Metab 284(2): E424-34
    › Primary publication · 12397027 (PubMed)
  118. Direct interaction of the inhibitory gamma-subunit of Rod cGMP phosphodiesterase (PDE6) with the PDE6 GAFa domains. Muradov KG, Granovsky AE, Schey KL, Artemyev NO (2002) Biochemistry 41(12): 3884-90
    › Primary publication · 11900530 (PubMed)
  119. Radioiodination of the stable metabolic fragment of bradykinin, RPPGF. Morinelli TA, Meier GP, Schey KL, Margolius HS (2001) Peptides 22(12): 2169-74
    › Primary publication · 11786206 (PubMed)
  120. Activation mechanism of Gi and Go by reactive oxygen species. Nishida M, Schey KL, Takagahara S, Kontani K, Katada T, Urano Y, Nagano T, Nagao T, Kurose H (2002) J Biol Chem 277(11): 9036-42
    › Primary publication · 11781308 (PubMed)
  121. Structural characterization of intact G protein gamma subunits by mass spectrometry. Schey KL, Busman M, Cook LA, Hamm HE, Hildebrandt JD (2002) Methods Enzymol : 586-97
    › Primary publication · 11771413 (PubMed)
  122. Characterization of deamidated G protein subunits. McIntire WE, Schey KL, Knapp DR, Dingus J, Hildebrandt JD (2002) Methods Enzymol : 481-505
    › Primary publication · 11771405 (PubMed)
  123. Separation and analysis of G protein gamma subunits. Cook LA, Wilcox MD, Dingus J, Schey KL, Hildebrandt JD (2002) Methods Enzymol : 209-33
    › Primary publication · 11771385 (PubMed)