Julie Bastarache
Last active: 5/31/2018

Profile

The focus of my laboratory is to understand the cellular and molecular regulation of the alveolar capillary barrier during acute infection and injury. I aim to uncover novel mechanisms that contribute to the development of barrier dysfunction in sepsis and ARDS with the ultimate goal of identifying new biologic targets and unique therapeutic approaches that can be translated to clinical practice. My research approach is translational, utilizing patient samples and clinical data, mouse models and cell lines to study fundamental biologic mechanisms that regulate lung responses to injury. My molecular focus is on cell-free hemoglobin and Tissue Factor and my disease focuses are ARDS and sepsis. I also have a special interest in the biologic underpinnings of gender differences in acute illness, sepsis and ARDS. My goal is to translate findings from these mechanistic studies to develop personalized care for the critically ill patients. Much of my work has focused on lung permeability as it is regulated by the epithelium and endothelium. I have extensive experience with mouse and cell culture models of lung injury and currently have over ten murine lung injury models established in my laboratory. My laboratory is currently funded with two R01’s studying the effects of cell-free hemoglobin in sepsis and ARDS, a VA Merit Review defining the non-coagulant and protective role of alveolar epithelial Tissue Factor and a Department of Defense grant to study the lung epithelial glycocalyx in exhaled breath from ARDS patients. In addition, I have several ongoing projects using the Synthetic Derivative and BioVU to understand clinical and genetic underpinnings of acute and chronic lung diseases. I am currently a mentor on Gordon Bernard, Timothy Blackwell’s and Eric Delpire's T32 training grants. I have mentored 21 students, residents and fellows and currently serve as mentor for both a clinical fellow on this T32 and a junior faculty member who currently holds three mentored grants including the prestigious Parker B. Francis Fellowship in Pulmonary Medicine. My lab is always open to interested trainees and scientists at all levels interested in exploring important biological and clinical questions using a multitude of basic and translational research tools.

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Epithelial Heparan Sulfate Contributes to Alveolar Barrier Function and Is Shed during Lung Injury. Haeger SM, Liu X, Han X, McNeil JB, Oshima K, McMurtry SA, Yang Y, Ouyang Y, Zhang F, Nozik-Grayck E, Zemans RL, Tuder RM, Bastarache JA, Linhardt RJ, Schmidt EP (2018) Am J Respir Cell Mol Biol 59(3): 363-374
    › Primary publication · 29584451 (PubMed) · PMC6189644 (PubMed Central)
  2. Cell-free hemoglobin promotes primary graft dysfunction through oxidative lung endothelial injury. Shaver CM, Wickersham N, McNeil JB, Nagata H, Miller A, Landstreet SR, Kuck JL, Diamond JM, Lederer DJ, Kawut SM, Palmer SM, Wille KM, Weinacker A, Lama VN, Crespo MM, Orens JB, Shah PD, Hage CA, Cantu E, Porteous MK, Dhillon G, McDyer J, Bastarache JA, Christie JD, Ware LB, Lung Transplant Outcomes Group (LTOG) (2018) JCI Insight 3(2)
    › Primary publication · 29367464 (PubMed) · PMC5821179 (PubMed Central)
  3. GBT1118, a compound that increases the oxygen affinity of hemoglobin, improves survival in murine hypoxic acute lung injury. Putz ND, Shaver CM, Dufu K, Li CM, Xu Q, Hutchaleelaha A, Lehrer-Graiwer J, Majka SM, Ware LB, Bastarache JA (2018) J Appl Physiol (1985) 124(4): 899-905
    › Primary publication · 29357510 (PubMed)
  4. Novel Method for Noninvasive Sampling of the Distal Airspace in Acute Respiratory Distress Syndrome. McNeil JB, Shaver CM, Kerchberger VE, Russell DW, Grove BS, Warren MA, Wickersham NE, Ware LB, McDonald WH, Bastarache JA (2018) Am J Respir Crit Care Med 197(8): 1027-1035
    › Primary publication · 29253353 (PubMed) · PMC5909163 (PubMed Central)
  5. Ascorbic acid attenuates endothelial permeability triggered by cell-free hemoglobin. Kuck JL, Bastarache JA, Shaver CM, Fessel JP, Dikalov SI, May JM, Ware LB (2018) Biochem Biophys Res Commun 495(1): 433-437
    › Primary publication · 29129689 (PubMed) · PMC5736437 (PubMed Central)
  6. Aspirin for Sepsis Prophylaxis: An Ounce of Prevention? Casey JD, Semler MW, Bastarache JA (2017) Crit Care Med 45(11): 1959-1960
    › Primary publication · 29028704 (PubMed) · PMC5658041 (PubMed Central)
  7. Endothelial glycocalyx degradation is more severe in patients with non-pulmonary sepsis compared to pulmonary sepsis and associates with risk of ARDS and other organ dysfunction. Murphy LS, Wickersham N, McNeil JB, Shaver CM, May AK, Bastarache JA, Ware LB (2017) Ann Intensive Care 7(1): 102
    › Primary publication · 28986821 (PubMed) · PMC5630541 (PubMed Central)
  8. Cell-Free Hemoglobin-mediated Increases in Vascular Permeability. A Novel Mechanism of Primary Graft Dysfunction and a New Therapeutic Target. Shaver CM, Wickersham N, McNeil JB, Nagata H, Sills G, Kuck JL, Janz DR, Bastarache JA, Ware LB (2017) Ann Am Thorac Soc 14(Supplement_3): S251-S252
    › Primary publication · 28945473 (PubMed) · PMC5711346 (PubMed Central)
  9. Oxygenation Saturation Index Predicts Clinical Outcomes in ARDS. DesPrez K, McNeil JB, Wang C, Bastarache JA, Shaver CM, Ware LB (2017) Chest 152(6): 1151-1158
    › Primary publication · 28823812 (PubMed) · PMC5812755 (PubMed Central)
  10. A common deletion in the haptoglobin gene associated with blood cholesterol levels among Chinese women. Zheng NS, Bastarache LA, Bastarache JA, Lu Y, Ware LB, Shu XO, Denny JC, Long J (2017) J Hum Genet 62(10): 911-914
    › Primary publication · 28659611 (PubMed) · PMC5824713 (PubMed Central)
  11. Prehospital aspirin use is associated with reduced risk of acute respiratory distress syndrome in critically ill patients: a propensity-adjusted analysis. Chen W, Janz DR, Bastarache JA, May AK, O'Neal HR, Bernard GR, Ware LB (2015) Crit Care Med 43(4): 801-7
    › Primary publication · 25559436 (PubMed) · PMC4359645 (PubMed Central)
  12. Randomized, placebo-controlled trial of acetaminophen for the reduction of oxidative injury in severe sepsis: the Acetaminophen for the Reduction of Oxidative Injury in Severe Sepsis trial. Janz DR, Bastarache JA, Rice TW, Bernard GR, Warren MA, Wickersham N, Sills G, Oates JA, Roberts LJ, Ware LB, Acetaminophen for the Reduction of Oxidative Injury in Severe Sepsis Study Group (2015) Crit Care Med 43(3): 534-41
    › Primary publication · 25474535 (PubMed) · PMC4336619 (PubMed Central)
  13. ARDS: new mechanistic insights, new therapeutic directions. Ware LB, Bastarache JA, Calfee CS (2014) Clin Chest Med 35(4): xv-xvi
    › Primary publication · 25453428 (PubMed)
  14. Clinical and biological heterogeneity in acute respiratory distress syndrome: direct versus indirect lung injury. Shaver CM, Bastarache JA (2014) Clin Chest Med 35(4): 639-53
    › Primary publication · 25453415 (PubMed) · PMC4254695 (PubMed Central)
  15. Elevation of plasma cell-free hemoglobin in pulmonary arterial hypertension. Brittain EL, Janz DR, Austin ED, Bastarache JA, Wheeler LA, Ware LB, Hemnes AR (2014) Chest 146(6): 1478-1485
    › Primary publication · 24945582 (PubMed) · PMC4251612 (PubMed Central)
  16. Validation of a multiplex electrochemiluminescent immunoassay platform in human and mouse samples. Bastarache JA, Koyama T, Wickersham NE, Ware LB (2014) J Immunol Methods : 13-23
    › Primary publication · 24768796 (PubMed) · PMC4120713 (PubMed Central)
  17. Thinking outside the cell: how cell-free hemoglobin can potentiate acute lung injury. Bastarache JA, Roberts LJ, Ware LB (2014) Am J Physiol Lung Cell Mol Physiol 306(3): L231-2
    › Primary publication · 24337924 (PubMed) · PMC3920200 (PubMed Central)
  18. Association between haptoglobin, hemopexin and mortality in adults with sepsis. Janz DR, Bastarache JA, Sills G, Wickersham N, May AK, Bernard GR, Ware LB (2013) Crit Care 17(6): R272
    › Primary publication · 24225252 (PubMed) · PMC4056258 (PubMed Central)
  19. Longer storage duration of red blood cells is associated with an increased risk of acute lung injury in patients with sepsis. Janz DR, Zhao Z, Koyama T, May AK, Bernard GR, Bastarache JA, Young PP, Ware LB (2013) Ann Intensive Care 3(1): 33
    › Primary publication · 24059842 (PubMed) · PMC3848804 (PubMed Central)
  20. Histoplasmomas of uncommon size. Richmond BW, Worrell JA, Bastarache JA, Gervich DH, Slattery WR, Loyd JE (2013) Chest 143(6): 1795-1798
    › Primary publication · 23732591 (PubMed)
  21. Mechanical stretch inhibits lipopolysaccharide-induced keratinocyte-derived chemokine and tissue factor expression while increasing procoagulant activity in murine lung epithelial cells. Sebag SC, Bastarache JA, Ware LB (2013) J Biol Chem 288(11): 7875-84
    › Primary publication · 23362270 (PubMed) · PMC3597825 (PubMed Central)
  22. Association between cell-free hemoglobin, acetaminophen, and mortality in patients with sepsis: an observational study. Janz DR, Bastarache JA, Peterson JF, Sills G, Wickersham N, May AK, Roberts LJ, Ware LB (2013) Crit Care Med 41(3): 784-90
    › Primary publication · 23314583 (PubMed) · PMC3578977 (PubMed Central)
  23. Cecal ligation model of sepsis in mice: new insights. Bastarache JA, Matthay MA (2013) Crit Care Med 41(1): 356-7
    › Primary publication · 23269150 (PubMed) · PMC3734801 (PubMed Central)
  24. Low levels of tissue factor lead to alveolar haemorrhage, potentiating murine acute lung injury and oxidative stress. Bastarache JA, Sebag SC, Clune JK, Grove BS, Lawson WE, Janz DR, Roberts LJ, Dworski R, Mackman N, Ware LB (2012) Thorax 67(12): 1032-9
    › Primary publication · 23033361 (PubMed) · PMC3652432 (PubMed Central)
  25. The severity of shock is associated with impaired rates of net alveolar fluid clearance in clinical acute lung injury. Zeyed YF, Bastarache JA, Matthay MA, Ware LB (2012) Am J Physiol Lung Cell Mol Physiol 303(6): L550-5
    › Primary publication · 22821995 (PubMed) · PMC3468480 (PubMed Central)
  26. Postoperative estradiol levels associate with development of primary graft dysfunction in lung transplantation patients. Bastarache JA, Diamond JM, Kawut SM, Lederer DJ, Ware LB, Christie JD (2012) Gend Med 9(3): 154-65
    › Primary publication · 22361838 (PubMed) · PMC3374892 (PubMed Central)
  27. Markers of inflammation and coagulation may be modulated by enteral feeding strategy. Bastarache JA, Ware LB, Girard TD, Wheeler AP, Rice TW (2012) JPEN J Parenter Enteral Nutr 36(6): 732-40
    › Primary publication · 22318965 (PubMed) · PMC3726194 (PubMed Central)
  28. Interferon-γ and tumor necrosis factor-α act synergistically to up-regulate tissue factor in alveolar epithelial cells. Bastarache JA, Sebag SC, Grove BS, Ware LB (2011) Exp Lung Res 37(8): 509-17
    › Primary publication · 21913843 (PubMed) · PMC3652424 (PubMed Central)
  29. Therapeutic modulation of coagulation and fibrinolysis in acute lung injury and the acute respiratory distress syndrome. Sebag SC, Bastarache JA, Ware LB (2011) Curr Pharm Biotechnol 12(9): 1481-96
    › Primary publication · 21401517 (PubMed) · PMC3893117 (PubMed Central)
  30. Accuracy and reproducibility of a multiplex immunoassay platform: a validation study. Bastarache JA, Koyama T, Wickersham NE, Mitchell DB, Mernaugh RL, Ware LB (2011) J Immunol Methods 367(1-2): 33-9
    › Primary publication · 21277854 (PubMed) · PMC3108329 (PubMed Central)
  31. Alveolar fluid clearance is faster in women with acute lung injury compared to men. Bastarache JA, Ong T, Matthay MA, Ware LB (2011) J Crit Care 26(3): 249-56
    › Primary publication · 20688464 (PubMed) · PMC2982881 (PubMed Central)
  32. Determining the aetiology of pulmonary oedema by the oedema fluid-to-plasma protein ratio. Ware LB, Fremont RD, Bastarache JA, Calfee CS, Matthay MA (2010) Eur Respir J 35(2): 331-7
    › Primary publication · 19741024 (PubMed) · PMC2819058 (PubMed Central)
  33. Procoagulant alveolar microparticles in the lungs of patients with acute respiratory distress syndrome. Bastarache JA, Fremont RD, Kropski JA, Bossert FR, Ware LB (2009) Am J Physiol Lung Cell Mol Physiol 297(6): L1035-41
    › Primary publication · 19700643 (PubMed) · PMC2793184 (PubMed Central)
  34. Development of animal models for the acute respiratory distress syndrome. Bastarache JA, Blackwell TS (2009) Dis Model Mech 2(5-6): 218-23
    › Primary publication · 19407329 (PubMed) · PMC2675821 (PubMed Central)
  35. The complex role of fibrin in acute lung injury. Bastarache JA (2009) Am J Physiol Lung Cell Mol Physiol 296(3): L275-6
    › Primary publication · 19118088 (PubMed)
  36. The coagulation cascade in sepsis. Wang L, Bastarache JA, Ware LB (2008) Curr Pharm Des 14(19): 1860-9
    › Primary publication · 18691097 (PubMed)
  37. Intra-alveolar tissue factor pathway inhibitor is not sufficient to block tissue factor procoagulant activity. Bastarache JA, Wang L, Wang Z, Albertine KH, Matthay MA, Ware LB (2008) Am J Physiol Lung Cell Mol Physiol 294(5): L874-81
    › Primary publication · 18310227 (PubMed) · PMC2764534 (PubMed Central)
  38. Computed tomography and the idiopathic form of proliferative fibrosing mediastinitis. Worrell JA, Donnelly EF, Martin JB, Bastarache JA, Loyd JE (2007) J Thorac Imaging 22(3): 235-40
    › Primary publication · 17721332 (PubMed)
  39. Acute lung injury edema fluid decreases net fluid transport across human alveolar epithelial type II cells. Lee JW, Fang X, Dolganov G, Fremont RD, Bastarache JA, Ware LB, Matthay MA (2007) J Biol Chem 282(33): 24109-19
    › Primary publication · 17580309 (PubMed) · PMC2765119 (PubMed Central)
  40. The alveolar epithelium can initiate the extrinsic coagulation cascade through expression of tissue factor. Bastarache JA, Wang L, Geiser T, Wang Z, Albertine KH, Matthay MA, Ware LB (2007) Thorax 62(7): 608-16
    › Primary publication · 17356058 (PubMed) · PMC2117249 (PubMed Central)
  41. Novel role of the human alveolar epithelium in regulating intra-alveolar coagulation. Wang L, Bastarache JA, Wickersham N, Fang X, Matthay MA, Ware LB (2007) Am J Respir Cell Mol Biol 36(4): 497-503
    › Primary publication · 17099142 (PubMed) · PMC1899324 (PubMed Central)
  42. The role of the coagulation cascade in the continuum of sepsis and acute lung injury and acute respiratory distress syndrome. Bastarache JA, Ware LB, Bernard GR (2006) Semin Respir Crit Care Med 27(4): 365-76
    › Primary publication · 16909370 (PubMed)
  43. Coagulation and fibrinolysis in human acute lung injury--new therapeutic targets? Ware LB, Bastarache JA, Wang L (2005) Keio J Med 54(3): 142-9
    › Primary publication · 16237276 (PubMed)