Claus Schneider
Last active: 4/5/2016

Profile

We are studying the biochemistry of lipid signaling. We are interested in how lipid signaling hormones are formed and what their roles are in human health and disease. 1. Discovery and characterization of novel lipid mediators The cyclooxygenase and lipoxygenase enzymes catalyze the first step in the formation of a diverse array of lipid mediators (“eicosanoids”) from arachidonic acid. We are interested in exploring novel pathways of how these mediators are formed, and to establish their role in the progression of inflammatory diseases like atherosclerosis or cancer. 2. Mechanisms of oxygenation of fatty acids Lipid peroxidation and oxidative stress are elementary pathophysiological processes involved in the progression of many diseases, e.g., atherosclerosis, Alzheimer's, and cardiovascular diseases. The lipid aldehyde, 4-hydroxynonenal (HNE), has been recognized as one of the most cytotoxic products formed during lipid peroxidation almost three decades ago. Yet despite the wealth of information on its pathological properties, only little is known about its mechanism(s) of formation. We are using model autoxidation reactions with common and unusual fatty acid hydroperoxide precursors to identify mechanistically significant intermediates that shed a light on the pathways of formation of HNE. The products are analyzed using LC-MS, NMR, GC-MS, UV, and CD spectroscopy. Part of this research is carried out in collaboration with Ned Porters group in the Department of Chemistry. 3. Curcumin as a chemopreventive agent Curcumin is the spice that gives curry its yellow color. It has also been used in traditional Asian medicine as an anti-inflammatory and wound-healing agent. In the past few years, curcumin has received increasing attention for use as a dietary cancer chemopreventive agent. We are studying the oxidative metabolism of curcumin, and we are testing the hypothesis that oxidative metabolites of curcumin are contributing to its chemopreventive activity.

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Degradation of Curcumin: From Mechanism to Biological Implications. Schneider C, Gordon ON, Edwards RL, Luis PB (2015) J Agric Food Chem 63(35): 7606-14
    › Primary publication · 25817068 (PubMed) · PMC4752206 (PubMed Central)
  2. Oxidative Transformation of Demethoxy- and Bisdemethoxycurcumin: Products, Mechanism of Formation, and Poisoning of Human Topoisomerase IIα. Gordon ON, Luis PB, Ashley RE, Osheroff N, Schneider C (2015) Chem Res Toxicol 28(5): 989-96
    › Primary publication · 25806475 (PubMed) · PMC4437832 (PubMed Central)
  3. Unraveling curcumin degradation: autoxidation proceeds through spiroepoxide and vinylether intermediates en route to the main bicyclopentadione. Gordon ON, Luis PB, Sintim HO, Schneider C (2015) J Biol Chem 290(8): 4817-28
    › Primary publication · 25564617 (PubMed) · PMC4335222 (PubMed Central)
  4. Cyclooxygenase-2 catalysis and inhibition in lipid bilayer nanodiscs. Orlando BJ, McDougle DR, Lucido MJ, Eng ET, Graham LA, Schneider C, Stokes DL, Das A, Malkowski MG (2014) Arch Biochem Biophys : 33-40
    › Primary publication · 24503478 (PubMed) · PMC3967765 (PubMed Central)
  5. Facile synthesis of deuterated and [(14) C]labeled analogs of vanillin and curcumin for use as mechanistic and analytical tools. Gordon ON, Graham LA, Schneider C (2013) J Labelled Comp Radiopharm 56(14): 696-9
    › Primary publication · 24339007 (PubMed) · PMC3943166 (PubMed Central)
  6. Isolation and characterization of two geometric allene oxide isomers synthesized from 9S-hydroperoxylinoleic acid by cytochrome P450 CYP74C3: stereochemical assignment of natural fatty acid allene oxides. Brash AR, Boeglin WE, Stec DF, Voehler M, Schneider C, Cha JK (2013) J Biol Chem 288(29): 20797-806
    › Primary publication · 23709224 (PubMed) · PMC3774351 (PubMed Central)
  7. Oxidative metabolites of curcumin poison human type II topoisomerases. Ketron AC, Gordon ON, Schneider C, Osheroff N (2013) Biochemistry 52(1): 221-7
    › Primary publication · 23253398 (PubMed) · PMC3541001 (PubMed Central)
  8. Transactivation of EGFR by LPS induces COX-2 expression in enterocytes. McElroy SJ, Hobbs S, Kallen M, Tejera N, Rosen MJ, Grishin A, Matta P, Schneider C, Upperman J, Ford H, Polk DB, Weitkamp JH (2012) PLoS One 7(5): e38373
    › Primary publication · 22675459 (PubMed) · PMC3364993 (PubMed Central)
  9. COX-2-dependent and -independent biosynthesis of dihydroxy-arachidonic acids in activated human leukocytes. Tejera N, Boeglin WE, Suzuki T, Schneider C (2012) J Lipid Res 53(1): 87-94
    › Primary publication · 22068350 (PubMed) · PMC3243484 (PubMed Central)
  10. Cyclooxygenases and lipoxygenases in cancer. Schneider C, Pozzi A (2011) Cancer Metastasis Rev 30(3-4): 277-94
    › Primary publication · 22002716 (PubMed) · PMC3798028 (PubMed Central)
  11. Applications of stereospecifically-labeled Fatty acids in oxygenase and desaturase biochemistry. Brash AR, Schneider C, Hamberg M (2012) Lipids 47(2): 101-16
    › Primary publication · 21971646 (PubMed) · PMC3315059 (PubMed Central)
  12. Biosynthesis of hemiketal eicosanoids by cross-over of the 5-lipoxygenase and cyclooxygenase-2 pathways. Griesser M, Suzuki T, Tejera N, Mont S, Boeglin WE, Pozzi A, Schneider C (2011) Proc Natl Acad Sci U S A 108(17): 6945-50
    › Primary publication · 21482803 (PubMed) · PMC3084063 (PubMed Central)
  13. Role of iPLA(2) in the regulation of Src trafficking and microglia chemotaxis. Lee SH, Schneider C, Higdon AN, Darley-Usmar VM, Chung CY (2011) Traffic 12(7): 878-89
    › Primary publication · 21438970 (PubMed) · PMC3115410 (PubMed Central)
  14. Autoxidative and cyclooxygenase-2 catalyzed transformation of the dietary chemopreventive agent curcumin. Griesser M, Pistis V, Suzuki T, Tejera N, Pratt DA, Schneider C (2011) J Biol Chem 286(2): 1114-24
    › Primary publication · 21071447 (PubMed) · PMC3020718 (PubMed Central)
  15. Formation of 4-hydroxynonenal from cardiolipin oxidation: Intramolecular peroxyl radical addition and decomposition. Liu W, Porter NA, Schneider C, Brash AR, Yin H (2011) Free Radic Biol Med 50(1): 166-78
    › Primary publication · 21047551 (PubMed) · PMC3014443 (PubMed Central)
  16. Identification and absolute configuration of dihydroxy-arachidonic acids formed by oxygenation of 5S-HETE by native and aspirin-acetylated COX-2. Mulugeta S, Suzuki T, Hernandez NT, Griesser M, Boeglin WE, Schneider C (2010) J Lipid Res 51(3): 575-85
    › Primary publication · 19752399 (PubMed) · PMC2817587 (PubMed Central)
  17. Convergence of the 5-LOX and COX-2 pathways: heme-catalyzed cleavage of the 5S-HETE-derived di-endoperoxide into aldehyde fragments. Griesser M, Boeglin WE, Suzuki T, Schneider C (2009) J Lipid Res 50(12): 2455-62
    › Primary publication · 19553698 (PubMed) · PMC2781317 (PubMed Central)
  18. Evidence for an ionic intermediate in the transformation of fatty acid hydroperoxide by a catalase-related allene oxide synthase from the Cyanobacterium Acaryochloris marina. Gao B, Boeglin WE, Zheng Y, Schneider C, Brash AR (2009) J Biol Chem 284(33): 22087-98
    › Primary publication · 19531485 (PubMed) · PMC2755933 (PubMed Central)
  19. Biosynthesis of a linoleic acid allylic epoxide: mechanistic comparison with its chemical synthesis and leukotriene A biosynthesis. Niisuke K, Boeglin WE, Murray JJ, Schneider C, Brash AR (2009) J Lipid Res 50(7): 1448-55
    › Primary publication · 19244216 (PubMed) · PMC2694342 (PubMed Central)
  20. An update on products and mechanisms of lipid peroxidation. Schneider C (2009) Mol Nutr Food Res 53(3): 315-21
    › Primary publication · 19006094 (PubMed) · PMC2868362 (PubMed Central)
  21. Intermolecular peroxyl radical reactions during autoxidation of hydroxy and hydroperoxy arachidonic acids generate a novel series of epoxidized products. Schneider C, Boeglin WE, Yin H, Porter NA, Brash AR (2008) Chem Res Toxicol 21(4): 895-903
    › Primary publication · 18324788 (PubMed)
  22. Routes to 4-hydroxynonenal: fundamental issues in the mechanisms of lipid peroxidation. Schneider C, Porter NA, Brash AR (2008) J Biol Chem 283(23): 15539-43
    › Primary publication · 18285327 (PubMed) · PMC2414272 (PubMed Central)
  23. A 49-kDa mini-lipoxygenase from Anabaena sp. PCC 7120 retains catalytically complete functionality. Zheng Y, Boeglin WE, Schneider C, Brash AR (2008) J Biol Chem 283(8): 5138-47
    › Primary publication · 18070874 (PubMed)
  24. Enzymatic synthesis of a bicyclobutane fatty acid by a hemoprotein lipoxygenase fusion protein from the cyanobacterium Anabaena PCC 7120. Schneider C, Niisuke K, Boeglin WE, Voehler M, Stec DF, Porter NA, Brash AR (2007) Proc Natl Acad Sci U S A 104(48): 18941-5
    › Primary publication · 18025466 (PubMed) · PMC2141887 (PubMed Central)
  25. Enantiomeric separation of hydroxy and hydroperoxy eicosanoids by chiral column chromatography. Schneider C, Yu Z, Boeglin WE, Zheng Y, Brash AR (2007) Methods Enzymol : 145-57
    › Primary publication · 17954233 (PubMed)
  26. The hepoxilin connection in the epidermis. Brash AR, Yu Z, Boeglin WE, Schneider C (2007) FEBS J 274(14): 3494-3502
    › Primary publication · 17608720 (PubMed)
  27. Control of oxygenation in lipoxygenase and cyclooxygenase catalysis. Schneider C, Pratt DA, Porter NA, Brash AR (2007) Chem Biol 14(5): 473-88
    › Primary publication · 17524979 (PubMed) · PMC2692746 (PubMed Central)
  28. Epidermal lipoxygenase products of the hepoxilin pathway selectively activate the nuclear receptor PPARalpha. Yu Z, Schneider C, Boeglin WE, Brash AR (2007) Lipids 42(6): 491-7
    › Primary publication · 17436029 (PubMed)
  29. Human and mouse eLOX3 have distinct substrate specificities: implications for their linkage with lipoxygenases in skin. Yu Z, Schneider C, Boeglin WE, Brash AR (2006) Arch Biochem Biophys 455(2): 188-96
    › Primary publication · 17045234 (PubMed) · PMC2636205 (PubMed Central)
  30. Molecular dynamics simulations of arachidonic acid-derived pentadienyl radical intermediate complexes with COX-1 and COX-2: insights into oxygenation regio- and stereoselectivity. Furse KE, Pratt DA, Schneider C, Brash AR, Porter NA, Lybrand TP (2006) Biochemistry 45(10): 3206-18
    › Primary publication · 16519515 (PubMed) · PMC2504531 (PubMed Central)
  31. Synthesis of dihydroperoxides of linoleic and linolenic acids and studies on their transformation to 4-hydroperoxynonenal. Schneider C, Boeglin WE, Yin H, Ste DF, Hachey DL, Porter NA, Brash AR (2005) Lipids 40(11): 1155-62
    › Primary publication · 16459928 (PubMed)
  32. Convergent oxygenation of arachidonic acid by 5-lipoxygenase and cyclooxygenase-2. Schneider C, Boeglin WE, Yin H, Stec DF, Voehler M (2006) J Am Chem Soc 128(3): 720-1
    › Primary publication · 16417352 (PubMed) · PMC2532595 (PubMed Central)
  33. On the relationships of substrate orientation, hydrogen abstraction, and product stereochemistry in single and double dioxygenations by soybean lipoxygenase-1 and its Ala542Gly mutant. Coffa G, Imber AN, Maguire BC, Laxmikanthan G, Schneider C, Gaffney BJ, Brash AR (2005) J Biol Chem 280(46): 38756-66
    › Primary publication · 16157595 (PubMed) · PMC1351262 (PubMed Central)
  34. A comprehensive model of positional and stereo control in lipoxygenases. Coffa G, Schneider C, Brash AR (2005) Biochem Biophys Res Commun 338(1): 87-92
    › Primary publication · 16111652 (PubMed)
  35. Detection and subcellular localization of two 15S-lipoxygenases in human cornea. Chang MS, Schneider C, Roberts RL, Shappell SB, Haselton FR, Boeglin WE, Brash AR (2005) Invest Ophthalmol Vis Sci 46(3): 849-56
    › Primary publication · 15728540 (PubMed)
  36. Mutations associated with a congenital form of ichthyosis (NCIE) inactivate the epidermal lipoxygenases 12R-LOX and eLOX3. Yu Z, Schneider C, Boeglin WE, Brash AR (2005) Biochim Biophys Acta 1686(3): 238-47
    › Primary publication · 15629692 (PubMed)
  37. Chemistry and biology of vitamin E. Schneider C (2005) Mol Nutr Food Res 49(1): 7-30
    › Primary publication · 15580660 (PubMed)
  38. Human cyclo-oxygenase-1 and an alternative splice variant: contrasts in expression of mRNA, protein and catalytic activities. Schneider C, Boeglin WE, Brash AR (2005) Biochem J 385(Pt 1): 57-64
    › Primary publication · 15361066 (PubMed) · PMC1134673 (PubMed Central)
  39. Autoxidative transformation of chiral omega6 hydroxy linoleic and arachidonic acids to chiral 4-hydroxy-2E-nonenal. Schneider C, Porter NA, Brash AR (2004) Chem Res Toxicol 17(7): 937-41
    › Primary publication · 15257619 (PubMed)
  40. Induction of apoptosis in colorectal carcinoma cells treated with 4-hydroxy-2-nonenal and structurally related aldehydic products of lipid peroxidation. West JD, Ji C, Duncan ST, Amarnath V, Schneider C, Rizzo CJ, Brash AR, Marnett LJ (2004) Chem Res Toxicol 17(4): 453-62
    › Primary publication · 15089087 (PubMed)
  41. Upregulation of 8-lipoxygenase in the dermatitis of IkappaB-alpha-deficient mice. Schneider C, Strayhorn WD, Brantley DM, Nanney LB, Yull FE, Brash AR (2004) J Invest Dermatol 122(3): 691-8
    › Primary publication · 15086555 (PubMed)
  42. Identification of two cyclooxygenase active site residues, Leucine 384 and Glycine 526, that control carbon ring cyclization in prostaglandin biosynthesis. Schneider C, Boeglin WE, Brash AR (2004) J Biol Chem 279(6): 4404-14
    › Primary publication · 14594816 (PubMed)
  43. The lipoxygenase gene ALOXE3 implicated in skin differentiation encodes a hydroperoxide isomerase. Yu Z, Schneider C, Boeglin WE, Marnett LJ, Brash AR (2003) Proc Natl Acad Sci U S A 100(16): 9162-7
    › Primary publication · 12881489 (PubMed) · PMC170889 (PubMed Central)
  44. Analysis of cyclooxygenase-substrate interactions using stereospecifically-labeled arachidonic acids. Schneider C, Boeglin WE, Brash AR (2002) Adv Exp Med Biol : 49-53
    › Primary publication · 12664563 (PubMed)
  45. Lipoxygenase-catalyzed formation of R-configuration hydroperoxides. Schneider C, Brash AR (2002) Prostaglandins Other Lipid Mediat : 291-301
    › Primary publication · 12432924 (PubMed)
  46. Detection of the 15-acetate of prostaglandin E2 methyl ester as a prominent component of the prostaglandins in the gorgonian coral Plexaura homomalla. Schneider C, Manier ML, Hachey DL, Brash AR (2002) Lipids 37(2): 217-21
    › Primary publication · 11908914 (PubMed)
  47. Control of prostaglandin stereochemistry at the 15-carbon by cyclooxygenases-1 and -2. A critical role for serine 530 and valine 349. Schneider C, Boeglin WE, Prusakiewicz JJ, Rowlinson SW, Marnett LJ, Samel N, Brash AR (2002) J Biol Chem 277(1): 478-85
    › Primary publication · 11677234 (PubMed)
  48. Amino acid determinants in cyclooxygenase-2 oxygenation of the endocannabinoid 2-arachidonylglycerol. Kozak KR, Prusakiewicz JJ, Rowlinson SW, Schneider C, Marnett LJ (2001) J Biol Chem 276(32): 30072-7
    › Primary publication · 11402053 (PubMed)
  49. Biogenesis of volatile aldehydes from fatty acid hydroperoxides: molecular cloning of a hydroperoxide lyase (CYP74C) with specificity for both the 9- and 13-hydroperoxides of linoleic and linolenic acids. Tijet N, Schneider C, Muller BL, Brash AR (2001) Arch Biochem Biophys 386(2): 281-9
    › Primary publication · 11368353 (PubMed)
  50. Detection and cellular localization of 12R-lipoxygenase in human tonsils. Schneider C, Keeney DS, Boeglin WE, Brash AR (2001) Arch Biochem Biophys 386(2): 268-74
    › Primary publication · 11368351 (PubMed)
  51. Two distinct pathways of formation of 4-hydroxynonenal. Mechanisms of nonenzymatic transformation of the 9- and 13-hydroperoxides of linoleic acid to 4-hydroxyalkenals. Schneider C, Tallman KA, Porter NA, Brash AR (2001) J Biol Chem 276(24): 20831-8
    › Primary publication · 11259420 (PubMed)
  52. 15S-Hydroxyeicosatetraenoic acid activates peroxisome proliferator-activated receptor gamma and inhibits proliferation in PC3 prostate carcinoma cells. Shappell SB, Gupta RA, Manning S, Whitehead R, Boeglin WE, Schneider C, Case T, Price J, Jack GS, Wheeler TM, Matusik RJ, Brash AR, Dubois RN (2001) Cancer Res 61(2): 497-503
    › Primary publication · 11212240 (PubMed)
  53. Enantiomeric separation of hydroxy eicosanoids by chiral column chromatography: effect of the alcohol modifier. Schneider C, Boeglin WE, Brash AR (2000) Anal Biochem 287(1): 186-9
    › Primary publication · 11078601 (PubMed)
  54. Synthesis and applications of stereospecifically (3)H-labeled arachidonic acids as mechanistic probes for lipoxygenase and cyclooxygenase catalysis. Schneider C, Boeglin WE, Lai S, Cha JK, Brash AR (2000) Anal Biochem 284(1): 125-35
    › Primary publication · 10933865 (PubMed)
  55. Spatial requirements for 15-(R)-hydroxy-5Z,8Z,11Z, 13E-eicosatetraenoic acid synthesis within the cyclooxygenase active site of murine COX-2. Why acetylated COX-1 does not synthesize 15-(R)-hete. Rowlinson SW, Crews BC, Goodwin DC, Schneider C, Gierse JK, Marnett LJ (2000) J Biol Chem 275(9): 6586-91
    › Primary publication · 10692466 (PubMed)
  56. Stereospecificity of hydrogen abstraction in the conversion of arachidonic acid to 15R-HETE by aspirin-treated cyclooxygenase-2. Implications for the alignment of substrate in the active site. Schneider C, Brash AR (2000) J Biol Chem 275(7): 4743-6
    › Primary publication · 10671506 (PubMed)