Ryoma Ohi
Faculty Member
Last active: 4/18/2017

Profile

Our group studies animal cell division. During this process, the microtubule cytoskeleton is organized into a transient structure called the mitotic spindle. This apparatus attaches replicated chromosomes via kinetochores and generates forces that power the division of chromosomes among two daughter cells. We apply a multidisciplinary approach to examine the process of mitotic spindle assembly and the interface between kinetochores and microtubules. We use quantitative light microscopy to investigate protein behavior in the mitotic spindle, biochemistry to understand the function of key mitotic regulators, and small molecules to perturb spindle function. Our long term goal is to use our findings to develop new anti-mitotic oncological therapies. Currently, our lab is studying how several families of kinesin-like proteins impact spindle assembly and function in animal cells. We are particularly interested in the roles of the kinesin-8s at spindle microtubule plus-ends, the mechanisms by which the kinesin-5 and kinesin-12 motors enforce spindle bipolarity, and the roles of kinesin-13s during cytokinesis.

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Eg5 Inhibitors Have Contrasting Effects on Microtubule Stability and Metaphase Spindle Integrity. Chen GY, Kang YJ, Gayek AS, Youyen W, Tüzel E, Ohi R, Hancock WO (2017) ACS Chem Biol
    › Primary publication · 28165699 (PubMed)
  2. Resistance is not futile: Surviving Eg5 inhibition. Dumas ME, Sturgill EG, Ohi R (2016) Cell Cycle 15(21): 2845-2847
    › Primary publication · 27356105 (PubMed) · PMC5105924 (PubMed Central)
  3. CDK-1 Inhibition in G2 Stabilizes Kinetochore-Microtubules in the following Mitosis. Gayek AS, Ohi R (2016) PLoS One 11(6): e0157491
    › Primary publication · 27281342 (PubMed) · PMC4900577 (PubMed Central)
  4. Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities. Pfaltzgraff ER, Roth GM, Miller PM, Gintzig AG, Ohi R, Bader DM (2016) Mol Biol Cell 27(13): 1990-9
    › Primary publication · 27146114 (PubMed) · PMC4927273 (PubMed Central)
  5. Kinesin-5 inhibitor resistance is driven by kinesin-12. Sturgill EG, Norris SR, Guo Y, Ohi R (2016) J Cell Biol 213(2): 213-27
    › Primary publication · 27091450 (PubMed) · PMC5084272 (PubMed Central)
  6. Ahead of the Curve: New Insights into Microtubule Dynamics. Ohi R, Zanic M (2016) F1000Res
    › Primary publication · 26998244 (PubMed) · PMC4786905 (PubMed Central)
  7. Expansion and concatenation of non-muscle myosin IIA filaments drive cellular contractile system formation during interphase and mitosis. Fenix AM, Taneja N, Buttler CA, Lewis J, Van Engelenburg SB, Ohi R, Burnette DT (2016) Mol Biol Cell
    › Primary publication · 26960797 (PubMed) · PMC4850034 (PubMed Central)
  8. The Timing of Midzone Stabilization during Cytokinesis Depends on Myosin II Activity and an Interaction between INCENP and Actin. Landino J, Ohi R (2016) Curr Biol 26(5): 698-706
    › Primary publication · 26898472 (PubMed) · PMC4783181 (PubMed Central)
  9. Biased Brownian motion as a mechanism to facilitate nanometer-scale exploration of the microtubule plus end by a kinesin-8. Shin Y, Du Y, Collier SE, Ohi MD, Lang MJ, Ohi R (2015) Proc Natl Acad Sci U S A 112(29): E3826-35
    › Primary publication · 26150501 (PubMed) · PMC4517267 (PubMed Central)
  10. Fanning the flames of CIN. Gayek AS, Ohi R (2015) Cell Cycle 14(16): 2560
    › Primary publication · 25714682 (PubMed) · PMC5242322 (PubMed Central)
  11. Kinesin-12 Kif15 targets kinetochore fibers through an intrinsic two-step mechanism. Sturgill EG, Das DK, Takizawa Y, Shin Y, Collier SE, Ohi MD, Hwang W, Lang MJ, Ohi R (2014) Curr Biol 24(19): 2307-13
    › Primary publication · 25264249 (PubMed) · PMC4207087 (PubMed Central)
  12. Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5. Collier SE, Voehler M, Peng D, Ohi R, Gould KL, Reiter NJ, Ohi MD (2014) Biochemistry 53(41): 6439-51
    › Primary publication · 25263959 (PubMed) · PMC4204884 (PubMed Central)
  13. Kinetochore-microtubule stability governs the metaphase requirement for Eg5. Gayek AS, Ohi R (2014) Mol Biol Cell 25(13): 2051-60
    › Primary publication · 24807901 (PubMed) · PMC4072578 (PubMed Central)
  14. Move in for the kill: motile microtubule regulators. Su X, Ohi R, Pellman D (2012) Trends Cell Biol 22(11): 567-75
    › Primary publication · 22959403 (PubMed) · PMC3482944 (PubMed Central)