Joern-Hendrik Weitkamp
Last active: 4/11/2016

Profile

Our laboratory focuses on the development of intestinal immune regulation in preterm infants. Premature infants are at risk for severe inflammatory complications such as necrotizing enterocolitis (NEC). I am a board-certified Pediatrician with additional post-doctoral training in Pediatric Infectious Diseases and Neonatal-Perinatal Medicine. For my post-doctoral training I created new technologies to compare the genetic antibody repertoire in virus-specific B cells between infants and adults. I expanded my research to study the development of T regulatory cells in the intestine of premature infants. I developed the collaborative infrastructure for the efficient procurement of fresh intestinal surgical tissue specimens from preterm infants with gastrointestinal pathology and by establishing methods for the analysis of single viable lymphocytes from these tissue sections. This surgical tissue repository has been a critical resource for human NEC tissue for numerous collaborators in the nation.  Our laboratory developed tools that for the first time permit the study of the ontogeny and function of immune effector and suppressor cells in the premature intestine. To overcome the ethical and technical limitations of human immunology research in this population, we customized multicolor flow cytometry protocols and microfluidic cell trap devices to study single immune cells isolated from surgically resected tissue specimens from infants with and without NEC. Using simultaneous sampling of intestinal tissue and feces during surgical resection, we have now established how the intestinal microbiome differs between the mucosal surface and stool, reflective of the distinct biogeography of the neonatal gastrointestinal tract and determined the specific intestinal microbiome profile in preterm infants with NEC

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Intrauterine Growth Restriction Alters Mouse Intestinal Architecture during Development. Fung CM, White JR, Brown AS, Gong H, Weitkamp JH, Frey MR, McElroy SJ (2016) PLoS One 11(1): e0146542
    › Primary publication · 26745886 (PubMed) · PMC4706418 (PubMed Central)
  2. Heart rate characteristic index monitoring for bloodstream infection in an NICU: a 3-year experience. Coggins SA, Weitkamp JH, Grunwald L, Stark AR, Reese J, Walsh W, Wynn JL (2016) Arch Dis Child Fetal Neonatal Ed 101(4): F329-32
    › Primary publication · 26518312 (PubMed) · PMC4851911 (PubMed Central)
  3. A newborn with severe skeletal dysplasia. Maalouf FI, Coggins SA, Phillips JA, Stefanescu BM, Weitkamp JH (2016) Arch Dis Child Educ Pract Ed 101(3): 147
    › Primary publication · 26307691 (PubMed)
  4. Neonatal CD71+ Erythroid Cells Do Not Modify Murine Sepsis Mortality. Wynn JL, Scumpia PO, Stocks BT, Romano-Keeler J, Alrifai MW, Liu JH, Kim AS, Alford CE, Matta P, Weitkamp JH, Moore DJ (2015) J Immunol 195(3): 1064-70
    › Primary publication · 26101326 (PubMed) · PMC4506905 (PubMed Central)
  5. Oligodendroglial maldevelopment in the cerebellum after postnatal hyperoxia and its prevention by minocycline. Scheuer T, Brockmöller V, Blanco Knowlton M, Weitkamp JH, Ruhwedel T, Mueller S, Endesfelder S, Bührer C, Schmitz T (2015) Glia 63(10): 1825-39
    › Primary publication · 25964099 (PubMed) · PMC4534324 (PubMed Central)
  6. Infectious causes of necrotizing enterocolitis. Coggins SA, Wynn JL, Weitkamp JH (2015) Clin Perinatol 42(1): 133-54, ix
    › Primary publication · 25678001 (PubMed) · PMC4328138 (PubMed Central)
  7. Maternal influences on fetal microbial colonization and immune development. Romano-Keeler J, Weitkamp JH (2015) Pediatr Res 77(1-2): 189-95
    › Primary publication · 25310759 (PubMed) · PMC4289016 (PubMed Central)
  8. CD8αα⁺ innate-type lymphocytes in the intestinal epithelium mediate mucosal immunity. Van Kaer L, Algood HMS, Singh K, Parekh VV, Greer MJ, Piazuelo MB, Weitkamp JH, Matta P, Chaturvedi R, Wilson KT, Olivares-Villagómez D (2014) Immunity 41(3): 451-464
    › Primary publication · 25220211 (PubMed) · PMC4169715 (PubMed Central)
  9. Small intestinal intraepithelial TCRγδ+ T lymphocytes are present in the premature intestine but selectively reduced in surgical necrotizing enterocolitis. Weitkamp JH, Rosen MJ, Zhao Z, Koyama T, Geem D, Denning TL, Rock MT, Moore DJ, Halpern MD, Matta P, Denning PW (2014) PLoS One 9(6): e99042
    › Primary publication · 24905458 (PubMed) · PMC4048281 (PubMed Central)
  10. Early life establishment of site-specific microbial communities in the gut. Romano-Keeler J, Moore DJ, Wang C, Brucker RM, Fonnesbeck C, Slaughter JC, Li H, Curran DP, Meng S, Correa H, Lovvorn HN, Tang YW, Bordenstein S, George AL, Weitkamp JH (2014) Gut Microbes 5(2): 192-201
    › Primary publication · 24637795 (PubMed) · PMC4063844 (PubMed Central)
  11. Congenital radiocontrast agent in the neonatal gut. Huang TK, Reese J, Weitkamp JH, Stancombe BB (2014) J Pediatr 164(5): 1236-1236.e1
    › Primary publication · 24534573 (PubMed)
  12. Use of a computerized C-reactive protein (CRP) based sepsis evaluation in very low birth weight (VLBW) infants: a five-year experience. Coggins SA, Wynn JL, Hill ML, Slaughter JC, Ozdas-Weitkamp A, Jalloh O, Waitman LR, Carnevale RJ, Weitkamp JH (2013) PLoS One 8(11): e78602
    › Primary publication · 24244325 (PubMed) · PMC3823853 (PubMed Central)
  13. Incidence of catheter-related bloodstream infections in neonates following removal of peripherally inserted central venous catheters. Casner M, Hoesli SJ, Slaughter JC, Hill M, Weitkamp JH (2014) Pediatr Crit Care Med 15(1): 42-8
    › Primary publication · 24141656 (PubMed)
  14. More than a gut feeling: predicting surgical necrotising enterocolitis. Weitkamp JH (2014) Gut 63(8): 1205-6
    › Primary publication · 24064006 (PubMed) · PMC4032372 (PubMed Central)
  15. Medical decision support using machine learning for early detection of late-onset neonatal sepsis. Mani S, Ozdas A, Aliferis C, Varol HA, Chen Q, Carnevale R, Chen Y, Romano-Keeler J, Nian H, Weitkamp JH (2014) J Am Med Inform Assoc 21(2): 326-36
    › Primary publication · 24043317 (PubMed) · PMC3932458 (PubMed Central)
  16. Mapping the New World of Necrotizing Enterocolitis (NEC): Review and Opinion. Gordon P, Christensen R, Weitkamp JH, Maheshwari A (2012) EJ Neonatol Res 2(4): 145-172
    › Primary publication · 23730536 (PubMed) · PMC3666872 (PubMed Central)
  17. Abnormal heart rate characteristics before clinical diagnosis of necrotizing enterocolitis. Stone ML, Tatum PM, Weitkamp JH, Mukherjee AB, Attridge J, McGahren ED, Rodgers BM, Lake DE, Moorman JR, Fairchild KD (2013) J Perinatol 33(11): 847-50
    › Primary publication · 23722974 (PubMed) · PMC4026091 (PubMed Central)
  18. Polymicrogyria and congenital parvovirus b19 infection. Schulert GS, Walsh WF, Weitkamp JH (2011) AJP Rep 1(2): 105-10
    › Primary publication · 23705097 (PubMed) · PMC3653533 (PubMed Central)
  19. STAT6 deficiency ameliorates severity of oxazolone colitis by decreasing expression of claudin-2 and Th2-inducing cytokines. Rosen MJ, Chaturvedi R, Washington MK, Kuhnhein LA, Moore PD, Coggeshall SS, McDonough EM, Weitkamp JH, Singh AB, Coburn LA, Williams CS, Yan F, Van Kaer L, Peebles RS, Wilson KT (2013) J Immunol 190(4): 1849-58
    › Primary publication · 23303670 (PubMed) · PMC3563924 (PubMed Central)
  20. Smad7 inhibits autocrine expression of TGF-β2 in intestinal epithelial cells in baboon necrotizing enterocolitis. Namachivayam K, Blanco CL, MohanKumar K, Jagadeeswaran R, Vasquez M, McGill-Vargas L, Garzon SA, Jain SK, Gill RK, Freitag NE, Weitkamp JH, Seidner SR, Maheshwari A (2013) Am J Physiol Gastrointest Liver Physiol 304(2): G167-80
    › Primary publication · 23154975 (PubMed) · PMC3543645 (PubMed Central)
  21. Safety and effectiveness of meropenem in infants with suspected or complicated intra-abdominal infections. Cohen-Wolkowiez M, Poindexter B, Bidegain M, Weitkamp JH, Schelonka RL, Randolph DA, Ward RM, Wade K, Valencia G, Burchfield D, Arrieta A, Mehta V, Walsh M, Kantak A, Rasmussen M, Sullivan JE, Finer N, Rich W, Brozanski BS, van den Anker J, Blumer J, Laughon M, Watt KM, Kearns GL, Capparelli EV, Martz K, Berezny K, Benjamin DK, Smith PB, Meropenem Study Team (2012) Clin Infect Dis 55(11): 1495-502
    › Primary publication · 22955430 (PubMed) · PMC3491861 (PubMed Central)
  22. Transactivation of EGFR by LPS induces COX-2 expression in enterocytes. McElroy SJ, Hobbs S, Kallen M, Tejera N, Rosen MJ, Grishin A, Matta P, Schneider C, Upperman J, Ford H, Polk DB, Weitkamp JH (2012) PLoS One 7(5): e38373
    › Primary publication · 22675459 (PubMed) · PMC3364993 (PubMed Central)
  23. Innate Immunity in the Small Intestine of the Preterm Infant. McElroy SJ, Weitkamp JH (2011) Neoreviews 12(9): e517-e526
    › Primary publication · 22639551 (PubMed) · PMC3359837 (PubMed Central)