Joern-Hendrik Weitkamp
Last active: 4/16/2021

Profile

Our laboratory focuses on the development of intestinal immune regulation in preterm infants. Premature infants are at risk for severe inflammatory complications such as necrotizing enterocolitis (NEC). I am a board-certified Pediatrician with additional post-doctoral training in Pediatric Infectious Diseases and Neonatal-Perinatal Medicine. For my post-doctoral training I created new technologies to compare the genetic antibody repertoire in virus-specific B cells between infants and adults. I expanded my research to study the development of T regulatory cells in the intestine of premature infants. I developed the collaborative infrastructure for the efficient procurement of fresh intestinal surgical tissue specimens from preterm infants with gastrointestinal pathology and by establishing methods for the analysis of single viable lymphocytes from these tissue sections. This surgical tissue repository has been a critical resource for human NEC tissue for numerous collaborators in the nation.  Our laboratory developed tools that for the first time permit the study of the ontogeny and function of immune effector and suppressor cells in the premature intestine. To overcome the ethical and technical limitations of human immunology research in this population, we customized multicolor flow cytometry protocols and microfluidic cell trap devices to study single immune cells isolated from surgically resected tissue specimens from infants with and without NEC. Using simultaneous sampling of intestinal tissue and feces during surgical resection, we have now established how the intestinal microbiome differs between the mucosal surface and stool, reflective of the distinct biogeography of the neonatal gastrointestinal tract and determined the specific intestinal microbiome profile in preterm infants with NEC

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Randomized trial of azithromycin to eradicate Ureaplasma respiratory colonization in preterm infants: 2-year outcomes. Viscardi RM, Terrin ML, Magder LS, Davis NL, Dulkerian SJ, Waites KB, Allen M, Ajayi-Akintade A, Ambalavanan N, Kaufman DA, Donohue P, Tuttle DJ, Weitkamp JH (2021) Pediatr Res
    › Primary publication · 33658655 (PubMed) · PMC8413397 (PubMed Central)
  2. Gut-derived T cells might cause brain injury in NEC. Weitkamp JH (2021) Nat Rev Gastroenterol Hepatol 18(5): 291-292
    › Primary publication · 33633397 (PubMed)
  3. Blood metabolomics in infants enrolled in a dose escalation pilot trial of budesonide in surfactant. Ballard PL, Torgerson D, Wadhawan R, Hudak ML, Weitkamp JH, Harris J, Asselin J, Chapin C, Ballard RA, McEvoy CT (2021) Pediatr Res 90(4): 784-794
    › Primary publication · 33469180 (PubMed) · PMC7814527 (PubMed Central)
  4. Parental Factors Associated With the Decision to Participate in a Neonatal Clinical Trial. Weiss EM, Olszewski AE, Guttmann KF, Magnus BE, Li S, Shah AR, Juul SE, Wu YW, Ahmad KA, Bendel-Stenzel E, Isaza NA, Lampland AL, Mathur AM, Rao R, Riley D, Russell DG, Salih ZNI, Torr CB, Weitkamp JH, Anani UE, Chang T, Dudley J, Flibotte J, Havrilla EM, Kathen CM, O'Kane AC, Perez K, Stanley BJ, Wilfond BS, Shah SK (2021) JAMA Netw Open 4(1): e2032106
    › Primary publication · 33433595 (PubMed) · PMC7804922 (PubMed Central)
  5. Aerosolized Calfactant for Newborns With Respiratory Distress: A Randomized Trial. Cummings JJ, Gerday E, Minton S, Katheria A, Albert G, Flores-Torres J, Famuyide M, Lampland A, Guthrie S, Kuehn D, Weitkamp JH, Fort P, Abu Jawdeh EG, Ryan RM, Martin GC, Swanson JR, Mulrooney N, Eyal F, Gerstmann D, Kumar P, Wilding GE, Egan EA, AERO-02 STUDY INVESTIGATORS (2020) Pediatrics 146(5)
    › Primary publication · 33060258 (PubMed)
  6. The Role of Biomarkers in Suspected Neonatal Sepsis. Weitkamp JH (2021) Clin Infect Dis 73(2): e391-e393
    › Primary publication · 32881996 (PubMed)
  7. Case 3: Abdominal Distention in a Preterm Infant. Sanlorenzo LA, Grossarth S, Weitkamp JH (2020) Neoreviews 21(6): e411-e413
    › Primary publication · 32482704 (PubMed)
  8. Randomised trial of azithromycin to eradicate in preterm infants. Viscardi RM, Terrin ML, Magder LS, Davis NL, Dulkerian SJ, Waites KB, Ambalavanan N, Kaufman DA, Donohue P, Tuttle DJ, Weitkamp JH, Hassan HE, Eddington ND (2020) Arch Dis Child Fetal Neonatal Ed 105(6): 615-622
    › Primary publication · 32170033 (PubMed) · PMC7592356 (PubMed Central)
  9. Osteopontin and iCD8α Cells Promote Intestinal Intraepithelial Lymphocyte Homeostasis. Nazmi A, Greer MJ, Hoek KL, Piazuelo MB, Weitkamp JH, Olivares-Villagómez D (2020) J Immunol 204(7): 1968-1981
    › Primary publication · 32102904 (PubMed) · PMC7175606 (PubMed Central)
  10. Dose-escalation trial of budesonide in surfactant for prevention of bronchopulmonary dysplasia in extremely low gestational age high-risk newborns (SASSIE). McEvoy CT, Ballard PL, Ward RM, Rower JE, Wadhawan R, Hudak ML, Weitkamp JH, Harris J, Asselin J, Chapin C, Ballard RA (2020) Pediatr Res 88(4): 629-636
    › Primary publication · 32006953 (PubMed) · PMC7223897 (PubMed Central)
  11. Meningitis, urinary tract, and bloodstream infections in very low birth weight infants enrolled in a heart rate characteristics monitoring trial. Weitkamp JH, Aschner JL, Carlo WA, Bancalari E, Perez JA, Navarrete CT, Schelonka RL, Whit Walker M, Porcelli P, O'Shea TM, Palmer C, Grossarth S, Lake DE, Fairchild KD (2020) Pediatr Res 87(7): 1226-1230
    › Primary publication · 31801155 (PubMed) · PMC7255929 (PubMed Central)
  12. Procalcitonin versus C-reactive protein: review of kinetics and performance for diagnosis of neonatal sepsis. Eschborn S, Weitkamp JH (2019) J Perinatol 39(7): 893-903
    › Primary publication · 30926891 (PubMed)
  13. Distinct mucosal microbial communities in infants with surgical necrotizing enterocolitis correlate with age and antibiotic exposure. Romano-Keeler J, Shilts MH, Tovchigrechko A, Wang C, Brucker RM, Moore DJ, Fonnesbeck C, Meng S, Correa H, Lovvorn HN, Tang YW, Hooper L, Bordenstein SR, Das SR, Weitkamp JH (2018) PLoS One 13(10): e0206366
    › Primary publication · 30365522 (PubMed) · PMC6203398 (PubMed Central)
  14. Innate and adaptive immunity in necrotizing enterocolitis. Mara MA, Good M, Weitkamp JH (2018) Semin Fetal Neonatal Med 23(6): 394-399
    › Primary publication · 30146477 (PubMed) · PMC6269198 (PubMed Central)
  15. Variability in Immunization Practices for Preterm Infants. Gopal SH, Edwards KM, Creech B, Weitkamp JH (2018) Am J Perinatol 35(14): 1394-1398
    › Primary publication · 29883982 (PubMed)
  16. Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice. Martinez KA, Romano-Keeler J, Zackular JP, Moore DJ, Brucker RM, Hooper C, Meng S, Brown N, Mallal S, Reese J, Aronoff DM, Shin H, Dominguez-Bello MG, Weitkamp JH (2018) PLoS One 13(5): e0197439
    › Primary publication · 29771989 (PubMed) · PMC5957394 (PubMed Central)
  17. Why are preterm newborns at increased risk of infection? Collins A, Weitkamp JH, Wynn JL (2018) Arch Dis Child Fetal Neonatal Ed 103(4): F391-F394
    › Primary publication · 29382648 (PubMed) · PMC6013388 (PubMed Central)
  18. The Marshall Klaus Research Award and Tribute to a Trailblazing Neonatologist. Grossarth SN, Coggins SA, Tune A, Ariagno RL, Fanaroff AA, Weitkamp JH (2018) J Perinatol 38(3): 211-216
    › Primary publication · 29234145 (PubMed)
  19. Antimicrobial and Antibiofilm Activity of Human Milk Oligosaccharides against Streptococcus agalactiae, Staphylococcus aureus, and Acinetobacter baumannii. Ackerman DL, Craft KM, Doster RS, Weitkamp JH, Aronoff DM, Gaddy JA, Townsend SD (2018) ACS Infect Dis 4(3): 315-324
    › Primary publication · 29198102 (PubMed) · PMC6019282 (PubMed Central)
  20. Erythematous plaques and papules on a premature infant. Riemenschneider K, Redenius R, Reese J, Fine JD, Weitkamp JH, Tkaczyk E (2017) J Am Acad Dermatol 76(4): e111-e112
    › Primary publication · 29081565 (PubMed) · PMC5563474 (PubMed Central)
  21. Monitoring neonates for ototoxicity. Garinis AC, Kemph A, Tharpe AM, Weitkamp JH, McEvoy C, Steyger PS (2018) Int J Audiol 57(sup4): S41-S48
    › Primary publication · 28949262 (PubMed) · PMC5741535 (PubMed Central)
  22. In Utero Exposure to Histological Chorioamnionitis Primes the Exometabolomic Profiles of Preterm CD4 T Lymphocytes. Matta P, Sherrod SD, Marasco CC, Moore DJ, McLean JA, Weitkamp JH (2017) J Immunol 199(9): 3074-3085
    › Primary publication · 28947540 (PubMed) · PMC5659751 (PubMed Central)
  23. Congenital Cutaneous Candidiasis: Prompt Systemic Treatment Is Associated With Improved Outcomes in Neonates. Kaufman DA, Coggins SA, Zanelli SA, Weitkamp JH (2017) Clin Infect Dis 64(10): 1387-1395
    › Primary publication · 28158439 (PubMed)
  24. Surgical necrotizing enterocolitis. Robinson JR, Rellinger EJ, Hatch LD, Weitkamp JH, Speck KE, Danko M, Blakely ML (2017) Semin Perinatol 41(1): 70-79
    › Primary publication · 27836422 (PubMed) · PMC5777619 (PubMed Central)
  25. Oral colostrum priming shortens hospitalization without changing the immunomicrobial milieu. Romano-Keeler J, Azcarate-Peril MA, Weitkamp JH, Slaughter JC, McDonald WH, Meng S, Latuga MS, Wynn JL (2017) J Perinatol 37(1): 36-41
    › Primary publication · 27684425 (PubMed) · PMC5215726 (PubMed Central)
  26. Histological chorioamnionitis shapes the neonatal transcriptomic immune response. Weitkamp JH, Guthrie SO, Wong HR, Moldawer LL, Baker HV, Wynn JL (2016) Early Hum Dev : 1-6
    › Primary publication · 27318328 (PubMed) · PMC4947555 (PubMed Central)
  27. Answers to Epilogue questions. Maalouf FI, Coggins SA, Phillips JA, Stefanescu BM, Weitkamp JH (2016) Arch Dis Child Educ Pract Ed 101(3): 168
    › Primary publication · 27194776 (PubMed)
  28. Epithelial PIK3R1 (p85) and TP53 Regulate Survivin Expression during Adaptation to Ileocecal Resection. Cohran V, Managlia E, Bradford EM, Goretsky T, Li T, Katzman RB, Cheresh P, Brown JB, Hawkins J, Liu SXL, De Plaen IG, Weitkamp JH, Helmrath M, Zhang Z, Barrett TA (2016) Am J Pathol 186(7): 1837-1846
    › Primary publication · 27157990 (PubMed) · PMC4929398 (PubMed Central)
  29. Targeting IL-17A attenuates neonatal sepsis mortality induced by IL-18. Wynn JL, Wilson CS, Hawiger J, Scumpia PO, Marshall AF, Liu JH, Zharkikh I, Wong HR, Lahni P, Benjamin JT, Plosa EJ, Weitkamp JH, Sherwood ER, Moldawer LL, Ungaro R, Baker HV, Lopez MC, McElroy SJ, Colliou N, Mohamadzadeh M, Moore DJ (2016) Proc Natl Acad Sci U S A 113(19): E2627-35
    › Primary publication · 27114524 (PubMed) · PMC4868456 (PubMed Central)
  30. Intrauterine Growth Restriction Alters Mouse Intestinal Architecture during Development. Fung CM, White JR, Brown AS, Gong H, Weitkamp JH, Frey MR, McElroy SJ (2016) PLoS One 11(1): e0146542
    › Primary publication · 26745886 (PubMed) · PMC4706418 (PubMed Central)
  31. Heart rate characteristic index monitoring for bloodstream infection in an NICU: a 3-year experience. Coggins SA, Weitkamp JH, Grunwald L, Stark AR, Reese J, Walsh W, Wynn JL (2016) Arch Dis Child Fetal Neonatal Ed 101(4): F329-32
    › Primary publication · 26518312 (PubMed) · PMC4851911 (PubMed Central)
  32. A newborn with severe skeletal dysplasia. Maalouf FI, Coggins SA, Phillips JA, Stefanescu BM, Weitkamp JH (2016) Arch Dis Child Educ Pract Ed 101(3): 147
    › Primary publication · 26307691 (PubMed)
  33. Neonatal CD71+ Erythroid Cells Do Not Modify Murine Sepsis Mortality. Wynn JL, Scumpia PO, Stocks BT, Romano-Keeler J, Alrifai MW, Liu JH, Kim AS, Alford CE, Matta P, Weitkamp JH, Moore DJ (2015) J Immunol 195(3): 1064-70
    › Primary publication · 26101326 (PubMed) · PMC4506905 (PubMed Central)
  34. Oligodendroglial maldevelopment in the cerebellum after postnatal hyperoxia and its prevention by minocycline. Scheuer T, Brockmöller V, Blanco Knowlton M, Weitkamp JH, Ruhwedel T, Mueller S, Endesfelder S, Bührer C, Schmitz T (2015) Glia 63(10): 1825-39
    › Primary publication · 25964099 (PubMed) · PMC4534324 (PubMed Central)
  35. Infectious causes of necrotizing enterocolitis. Coggins SA, Wynn JL, Weitkamp JH (2015) Clin Perinatol 42(1): 133-54, ix
    › Primary publication · 25678001 (PubMed) · PMC4328138 (PubMed Central)
  36. Maternal influences on fetal microbial colonization and immune development. Romano-Keeler J, Weitkamp JH (2015) Pediatr Res 77(1-2): 189-95
    › Primary publication · 25310759 (PubMed) · PMC4289016 (PubMed Central)
  37. CD8αα⁺ innate-type lymphocytes in the intestinal epithelium mediate mucosal immunity. Van Kaer L, Algood HMS, Singh K, Parekh VV, Greer MJ, Piazuelo MB, Weitkamp JH, Matta P, Chaturvedi R, Wilson KT, Olivares-Villagómez D (2014) Immunity 41(3): 451-464
    › Primary publication · 25220211 (PubMed) · PMC4169715 (PubMed Central)
  38. Small intestinal intraepithelial TCRγδ+ T lymphocytes are present in the premature intestine but selectively reduced in surgical necrotizing enterocolitis. Weitkamp JH, Rosen MJ, Zhao Z, Koyama T, Geem D, Denning TL, Rock MT, Moore DJ, Halpern MD, Matta P, Denning PW (2014) PLoS One 9(6): e99042
    › Primary publication · 24905458 (PubMed) · PMC4048281 (PubMed Central)
  39. Early life establishment of site-specific microbial communities in the gut. Romano-Keeler J, Moore DJ, Wang C, Brucker RM, Fonnesbeck C, Slaughter JC, Li H, Curran DP, Meng S, Correa H, Lovvorn HN, Tang YW, Bordenstein S, George AL, Weitkamp JH (2014) Gut Microbes 5(2): 192-201
    › Primary publication · 24637795 (PubMed) · PMC4063844 (PubMed Central)
  40. Congenital radiocontrast agent in the neonatal gut. Huang TK, Reese J, Weitkamp JH, Stancombe BB (2014) J Pediatr 164(5): 1236-1236.e1
    › Primary publication · 24534573 (PubMed)
  41. Use of a computerized C-reactive protein (CRP) based sepsis evaluation in very low birth weight (VLBW) infants: a five-year experience. Coggins SA, Wynn JL, Hill ML, Slaughter JC, Ozdas-Weitkamp A, Jalloh O, Waitman LR, Carnevale RJ, Weitkamp JH (2013) PLoS One 8(11): e78602
    › Primary publication · 24244325 (PubMed) · PMC3823853 (PubMed Central)
  42. Incidence of catheter-related bloodstream infections in neonates following removal of peripherally inserted central venous catheters. Casner M, Hoesli SJ, Slaughter JC, Hill M, Weitkamp JH (2014) Pediatr Crit Care Med 15(1): 42-8
    › Primary publication · 24141656 (PubMed)
  43. More than a gut feeling: predicting surgical necrotising enterocolitis. Weitkamp JH (2014) Gut 63(8): 1205-6
    › Primary publication · 24064006 (PubMed) · PMC4032372 (PubMed Central)
  44. Medical decision support using machine learning for early detection of late-onset neonatal sepsis. Mani S, Ozdas A, Aliferis C, Varol HA, Chen Q, Carnevale R, Chen Y, Romano-Keeler J, Nian H, Weitkamp JH (2014) J Am Med Inform Assoc 21(2): 326-36
    › Primary publication · 24043317 (PubMed) · PMC3932458 (PubMed Central)
  45. Mapping the New World of Necrotizing Enterocolitis (NEC): Review and Opinion. Gordon P, Christensen R, Weitkamp JH, Maheshwari A (2012) EJ Neonatol Res 2(4): 145-172
    › Primary publication · 23730536 (PubMed) · PMC3666872 (PubMed Central)
  46. Abnormal heart rate characteristics before clinical diagnosis of necrotizing enterocolitis. Stone ML, Tatum PM, Weitkamp JH, Mukherjee AB, Attridge J, McGahren ED, Rodgers BM, Lake DE, Moorman JR, Fairchild KD (2013) J Perinatol 33(11): 847-50
    › Primary publication · 23722974 (PubMed) · PMC4026091 (PubMed Central)
  47. Polymicrogyria and congenital parvovirus b19 infection. Schulert GS, Walsh WF, Weitkamp JH (2011) AJP Rep 1(2): 105-10
    › Primary publication · 23705097 (PubMed) · PMC3653533 (PubMed Central)
  48. STAT6 deficiency ameliorates severity of oxazolone colitis by decreasing expression of claudin-2 and Th2-inducing cytokines. Rosen MJ, Chaturvedi R, Washington MK, Kuhnhein LA, Moore PD, Coggeshall SS, McDonough EM, Weitkamp JH, Singh AB, Coburn LA, Williams CS, Yan F, Van Kaer L, Peebles RS, Wilson KT (2013) J Immunol 190(4): 1849-58
    › Primary publication · 23303670 (PubMed) · PMC3563924 (PubMed Central)
  49. Smad7 inhibits autocrine expression of TGF-β2 in intestinal epithelial cells in baboon necrotizing enterocolitis. Namachivayam K, Blanco CL, MohanKumar K, Jagadeeswaran R, Vasquez M, McGill-Vargas L, Garzon SA, Jain SK, Gill RK, Freitag NE, Weitkamp JH, Seidner SR, Maheshwari A (2013) Am J Physiol Gastrointest Liver Physiol 304(2): G167-80
    › Primary publication · 23154975 (PubMed) · PMC3543645 (PubMed Central)
  50. Safety and effectiveness of meropenem in infants with suspected or complicated intra-abdominal infections. Cohen-Wolkowiez M, Poindexter B, Bidegain M, Weitkamp JH, Schelonka RL, Randolph DA, Ward RM, Wade K, Valencia G, Burchfield D, Arrieta A, Mehta V, Walsh M, Kantak A, Rasmussen M, Sullivan JE, Finer N, Rich W, Brozanski BS, van den Anker J, Blumer J, Laughon M, Watt KM, Kearns GL, Capparelli EV, Martz K, Berezny K, Benjamin DK, Smith PB, Meropenem Study Team (2012) Clin Infect Dis 55(11): 1495-502
    › Primary publication · 22955430 (PubMed) · PMC3491861 (PubMed Central)
  51. Transactivation of EGFR by LPS induces COX-2 expression in enterocytes. McElroy SJ, Hobbs S, Kallen M, Tejera N, Rosen MJ, Grishin A, Matta P, Schneider C, Upperman J, Ford H, Polk DB, Weitkamp JH (2012) PLoS One 7(5): e38373
    › Primary publication · 22675459 (PubMed) · PMC3364993 (PubMed Central)
  52. Innate Immunity in the Small Intestine of the Preterm Infant. McElroy SJ, Weitkamp JH (2011) Neoreviews 12(9): e517-e526
    › Primary publication · 22639551 (PubMed) · PMC3359837 (PubMed Central)