Our program in structural cell biology includes research to understand the mechanisms of action of multi-protein assemblies that process DNA. Current efforts are focused on establishing and understanding the various functional roles of replication protein A (RPA), a heterotrimeric protein of 70, 32 and 14 kDa subunits. RPA is the major single-strand DNA binding protein in the cell and has been shown to form an integral part of most DNA processing assemblies. Our studies involve proteomic mapping of interactions with other DNA processing proteins and 3D structure determination of protein and protein-DNA complexes. These studies are providing novel insights into the structural basis for mutational defects leading to cancer and the essential foundation for the development of anticancer therapies.
Signalling via protein ubiquitination plays an essential role in an increasing number of cellular processes. A series of ligases catalyze the activation and subsequent covalent attachment of ubiquitin to substrate proteins. The E3 class of ubiquitin ligases typically involves the coordinated activity of a group of proteins that form a multi-protein complex of substrate and E2 ligase recognition elements, scaffolds, and molecular bridges. Our reseach involves the study of a newly discovered ubiquitin ligase activity associated with the RNA spliceosome and of a novel calcium-regulated ubiquitination process that is required for proper embryogenesis and development. Our studies involve proteomic strategies to determine which members of the complex interact with eachother and 3D structure determination of the corresponding protein complexes. These results are revealing information about how protein ubiquitination functions as a signaling mechanism and how defects in E3 ligase proteins lead to malfunction in fundamental cellular processes.
Calcium and calcium-binding proteins (CaBPs) play a central role in intracellular signal transduction pathways and are associated with a wide-range of effects on health and disease. Our research is directed towards understanding the molecular basis for the cellular activities of CaBPs, particularly the transduction of calcium signals to cellular targets. The primary experimental approach involves the determination of 3D structures of specific CaBPs in the presence and absence of calcium and cellular targets, using NMR, X-ray crystallography and computational methods. These are complemented by protein engineering and small molecule design. Ultimately, we seek the ability to control binding properties and interactions with target proteins, thereby enabling the design of specific biological activities and therapeutic strategies relevant to CaBP-mediated disease. . . .


The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Calmodulin Mutations Associated with Heart Arrhythmia: A Status Report. Chazin WJ, Johnson CN (2020) Int J Mol Sci 21(4)
    › Primary publication · 32093079 (PubMed) · PMC7073091 (PubMed Central)
  2. A key interaction with RPA orients XPA in NER complexes. Topolska-Woś AM, Sugitani N, Cordoba JJ, Le Meur KV, Le Meur RA, Kim HS, Yeo JE, Rosenberg D, Hammel M, Schärer OD, Chazin WJ (2020) Nucleic Acids Res 48(4): 2173-2188
    › Primary publication · 31925419 (PubMed) · PMC7038936 (PubMed Central)
  3. The Immune Protein Calprotectin Impacts Clostridioides difficile Metabolism through Zinc Limitation. Lopez CA, Beavers WN, Weiss A, Knippel RJ, Zackular JP, Chazin W, Skaar EP (2019) mBio 10(6)
    › Primary publication · 31744916 (PubMed) · PMC6867894 (PubMed Central)
  4. Arachidonic Acid Kills Staphylococcus aureus through a Lipid Peroxidation Mechanism. Beavers WN, Monteith AJ, Amarnath V, Mernaugh RL, Roberts LJ, Chazin WJ, Davies SS, Skaar EP (2019) mBio 10(5)
    › Primary publication · 31575763 (PubMed) · PMC6775451 (PubMed Central)
  5. Genetic Mosaicism in Calmodulinopathy. Wren LM, Jiménez-Jáimez J, Al-Ghamdi S, Al-Aama JY, Bdeir A, Al-Hassnan ZN, Kuan JL, Foo RY, Potet F, Johnson CN, Aziz MC, Carvill GL, Kaski JP, Crotti L, Perin F, Monserrat L, Burridge PW, Schwartz PJ, Chazin WJ, Bhuiyan ZA, George AL (2019) Circ Genom Precis Med 12(9): 375-385
    › Primary publication · 31454269 (PubMed) · PMC6751013 (PubMed Central)
  6. The anti-parasitic agent suramin and several of its analogues are inhibitors of the DNA binding protein Mcm10. Paulson CN, John K, Baxley RM, Kurniawan F, Orellana K, Francis R, Sobeck A, Eichman BF, Chazin WJ, Aihara H, Georg GI, Hawkinson JE, Bielinsky AK (2019) Open Biol 9(8): 190117
    › Primary publication · 31409229 (PubMed) · PMC6731595 (PubMed Central)
  7. The novel interaction between Neisseria gonorrhoeae TdfJ and human S100A7 allows gonococci to subvert host zinc restriction. Maurakis S, Keller K, Maxwell CN, Pereira K, Chazin WJ, Criss AK, Cornelissen CN (2019) PLoS Pathog 15(8): e1007937
    › Primary publication · 31369630 (PubMed) · PMC6692053 (PubMed Central)
  8. A new approach to discovery of S100 protein heterodimers. Garcia V, Chazin WJ (2019) FEBS J 286(10): 1838-1840
    › Primary publication · 31050191 (PubMed) · PMC6527480 (PubMed Central)
  9. The Innate Immune Protein S100A9 Protects from T-Helper Cell Type 2-mediated Allergic Airway Inflammation. Palmer LD, Maloney KN, Boyd KL, Goleniewska AK, Toki S, Maxwell CN, Chazin WJ, Peebles RS, Newcomb DC, Skaar EP (2019) Am J Respir Cell Mol Biol 61(4): 459-468
    › Primary publication · 30943376 (PubMed) · PMC6775951 (PubMed Central)
  10. Multi-metal Restriction by Calprotectin Impacts De Novo Flavin Biosynthesis in Acinetobacter baumannii. Wang J, Lonergan ZR, Gonzalez-Gutierrez G, Nairn BL, Maxwell CN, Zhang Y, Andreini C, Karty JA, Chazin WJ, Trinidad JC, Skaar EP, Giedroc DP (2019) Cell Chem Biol 26(5): 745-755.e7
    › Primary publication · 30905682 (PubMed) · PMC6525019 (PubMed Central)
  11. An Acinetobacter baumannii, Zinc-Regulated Peptidase Maintains Cell Wall Integrity during Immune-Mediated Nutrient Sequestration. Lonergan ZR, Nairn BL, Wang J, Hsu YP, Hesse LE, Beavers WN, Chazin WJ, Trinidad JC, VanNieuwenhze MS, Giedroc DP, Skaar EP (2019) Cell Rep 26(8): 2009-2018.e6
    › Primary publication · 30784584 (PubMed) · PMC6441547 (PubMed Central)
  12. S100 Proteins in the Innate Immune Response to Pathogens. Kozlyuk N, Monteith AJ, Garcia V, Damo SM, Skaar EP, Chazin WJ (2019) Methods Mol Biol : 275-290
    › Primary publication · 30710280 (PubMed) · PMC6475579 (PubMed Central)
  13. Functional and structural similarity of human DNA primase [4Fe4S] cluster domain constructs. Holt ME, Salay LE, O'Brien E, Barton JK, Chazin WJ (2018) PLoS One 13(12): e0209345
    › Primary publication · 30562384 (PubMed) · PMC6298731 (PubMed Central)
  14. Yeast require redox switching in DNA primase. O'Brien E, Salay LE, Epum EA, Friedman KL, Chazin WJ, Barton JK (2018) Proc Natl Acad Sci U S A 115(52): 13186-13191
    › Primary publication · 30541886 (PubMed) · PMC6310810 (PubMed Central)
  15. Substrate Binding Regulates Redox Signaling in Human DNA Primase. O'Brien E, Holt ME, Salay LE, Chazin WJ, Barton JK (2018) J Am Chem Soc 140(49): 17153-17162
    › Primary publication · 30433774 (PubMed) · PMC6470046 (PubMed Central)
  16. Characterization and development of SAPP as a specific peptidic inhibitor that targets Porphyromonas gingivalis. Ho MH, Lamont RJ, Chazin WJ, Chen H, Young DF, Kumar P, Xie H (2018) Mol Oral Microbiol 33(6): 430-439
    › Primary publication · 30298683 (PubMed) · PMC6246824 (PubMed Central)
  17. Disrupted structure and aberrant function of CHIP mediates the loss of motor and cognitive function in preclinical models of SCAR16. Shi CH, Rubel C, Soss SE, Sanchez-Hodge R, Zhang S, Madrigal SC, Ravi S, McDonough H, Page RC, Chazin WJ, Patterson C, Mao CY, Willis MS, Luo HY, Li YS, Stevens DA, Tang MB, Du P, Wang YH, Hu ZW, Xu YM, Schisler JC (2018) PLoS Genet 14(9): e1007664
    › Primary publication · 30222779 (PubMed) · PMC6160236 (PubMed Central)
  18. Antimicrobial action of calprotectin that does not involve metal withholding. Besold AN, Culbertson EM, Nam L, Hobbs RP, Boyko A, Maxwell CN, Chazin WJ, Marques AR, Culotta VC (2018) Metallomics 10(12): 1728-1742
    › Primary publication · 30206620 (PubMed) · PMC6417507 (PubMed Central)
  19. Calprotectin protects against experimental colonic inflammation in mice. Aranda CJ, Ocón B, Arredondo-Amador M, Suárez MD, Zarzuelo A, Chazin WJ, Martínez-Augustin O, Sánchez de Medina F (2018) Br J Pharmacol 175(19): 3797-3812
    › Primary publication · 30007036 (PubMed) · PMC6135788 (PubMed Central)
  20. A Mechanism of Calmodulin Modulation of the Human Cardiac Sodium Channel. Johnson CN, Potet F, Thompson MK, Kroncke BM, Glazer AM, Voehler MW, Knollmann BC, George AL, Chazin WJ (2018) Structure 26(5): 683-694.e3
    › Primary publication · 29606593 (PubMed) · PMC5932218 (PubMed Central)
  21. Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transfer. Bhuripanyo K, Wang Y, Liu X, Zhou L, Liu R, Duong D, Zhao B, Bi Y, Zhou H, Chen G, Seyfried NT, Chazin WJ, Kiyokawa H, Yin J (2018) Sci Adv 4(1): e1701393
    › Primary publication · 29326975 (PubMed) · PMC5756662 (PubMed Central)
  22. Role of Calprotectin in Withholding Zinc and Copper from Candida albicans. Besold AN, Gilston BA, Radin JN, Ramsoomair C, Culbertson EM, Li CX, Cormack BP, Chazin WJ, Kehl-Fie TE, Culotta VC (2018) Infect Immun 86(2)
    › Primary publication · 29133349 (PubMed) · PMC5778358 (PubMed Central)
  23. Ubiquitin turnover and endocytic trafficking in yeast are regulated by Ser57 phosphorylation of ubiquitin. Lee S, Tumolo JM, Ehlinger AC, Jernigan KK, Qualls-Histed SJ, Hsu PC, McDonald WH, Chazin WJ, MacGurn JA (2017) Elife
    › Primary publication · 29130884 (PubMed) · PMC5706963 (PubMed Central)
  24. Dietary Manganese Promotes Staphylococcal Infection of the Heart. Juttukonda LJ, Berends ETM, Zackular JP, Moore JL, Stier MT, Zhang Y, Schmitz JE, Beavers WN, Wijers CD, Gilston BA, Kehl-Fie TE, Atkinson J, Washington MK, Peebles RS, Chazin WJ, Torres VJ, Caprioli RM, Skaar EP (2017) Cell Host Microbe 22(4): 531-542.e8
    › Primary publication · 28943329 (PubMed) · PMC5638708 (PubMed Central)
  25. A Polymerase With Potential: The Fe-S Cluster in Human DNA Primase. Holt ME, Salay LE, Chazin WJ (2017) Methods Enzymol : 361-390
    › Primary publication · 28882207 (PubMed)
  26. Analysis of DNA binding by human factor xeroderma pigmentosum complementation group A (XPA) provides insight into its interactions with nucleotide excision repair substrates. Sugitani N, Voehler MW, Roh MS, Topolska-Woś AM, Chazin WJ (2017) J Biol Chem 292(41): 16847-16857
    › Primary publication · 28860187 (PubMed) · PMC5641873 (PubMed Central)
  27. RADX Promotes Genome Stability and Modulates Chemosensitivity by Regulating RAD51 at Replication Forks. Dungrawala H, Bhat KP, Le Meur R, Chazin WJ, Ding X, Sharan SK, Wessel SR, Sathe AA, Zhao R, Cortez D (2017) Mol Cell 67(3): 374-386.e5
    › Primary publication · 28735897 (PubMed) · PMC5548441 (PubMed Central)
  28. Response to Comments on "The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport". O'Brien E, Holt ME, Thompson MK, Salay LE, Ehlinger AC, Chazin WJ, Barton JK (2017) Science 357(6348)
    › Primary publication · 28729485 (PubMed) · PMC5935490 (PubMed Central)
  29. Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR. Thompson MK, Ehlinger AC, Chazin WJ (2017) Methods Enzymol : 49-76
    › Primary publication · 28668130 (PubMed)
  30. Calmodulin Mutants Linked to Catecholaminergic Polymorphic Ventricular Tachycardia Fail to Inhibit Human RyR2 Channels. Walweel K, Gomez-Hurtado N, Oo YW, Beard NA, Dos Remedios C, Johnson CN, Chazin WJ, van Helden DF, Knollmann BC, Laver DR (2017) J Am Coll Cardiol 70(1): 115-117
    › Primary publication · 28662798 (PubMed) · PMC7162611 (PubMed Central)
  31. Prp40 Homolog A Is a Novel Centrin Target. Díaz Casas A, Chazin WJ, Pastrana-Ríos B (2017) Biophys J 112(12): 2529-2539
    › Primary publication · 28636910 (PubMed) · PMC5479147 (PubMed Central)
  32. Molecular basis for PrimPol recruitment to replication forks by RPA. Guilliam TA, Brissett NC, Ehlinger A, Keen BA, Kolesar P, Taylor EM, Bailey LJ, Lindsay HD, Chazin WJ, Doherty AJ (2017) Nat Commun : 15222
    › Primary publication · 28534480 (PubMed) · PMC5457501 (PubMed Central)
  33. The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport. O'Brien E, Holt ME, Thompson MK, Salay LE, Ehlinger AC, Chazin WJ, Barton JK (2017) Science 355(6327)
    › Primary publication · 28232525 (PubMed) · PMC5338353 (PubMed Central)
  34. Erratum: Dietary zinc alters the microbiota and decreases resistance to Clostridium difficile infection. Zackular JP, Moore JL, Jordan AT, Juttukonda LJ, Noto MJ, Nicholson MR, Crews JD, Semler MW, Zhang Y, Ware LB, Washington MK, Chazin WJ, Caprioli RM, Skaar EP (2016) Nat Med 22(12): 1502
    › Primary publication · 27923021 (PubMed) · PMC5453636 (PubMed Central)
  35. ETAA1 acts at stalled replication forks to maintain genome integrity. Bass TE, Luzwick JW, Kavanaugh G, Carroll C, Dungrawala H, Glick GG, Feldkamp MD, Putney R, Chazin WJ, Cortez D (2016) Nat Cell Biol 18(11): 1185-1195
    › Primary publication · 27723720 (PubMed) · PMC5245861 (PubMed Central)
  36. Acinetobacter baumannii Coordinates Urea Metabolism with Metal Import To Resist Host-Mediated Metal Limitation. Juttukonda LJ, Chazin WJ, Skaar EP (2016) mBio 7(5)
    › Primary publication · 27677795 (PubMed) · PMC5050338 (PubMed Central)
  37. Dietary zinc alters the microbiota and decreases resistance to Clostridium difficile infection. Zackular JP, Moore JL, Jordan AT, Juttukonda LJ, Noto MJ, Nicholson MR, Crews JD, Semler MW, Zhang Y, Ware LB, Washington MK, Chazin WJ, Caprioli RM, Skaar EP (2016) Nat Med 22(11): 1330-1334
    › Primary publication · 27668938 (PubMed) · PMC5101143 (PubMed Central)
  38. Novel CPVT-Associated Calmodulin Mutation in CALM3 (CALM3-A103V) Activates Arrhythmogenic Ca Waves and Sparks. Gomez-Hurtado N, Boczek NJ, Kryshtal DO, Johnson CN, Sun J, Nitu FR, Cornea RL, Chazin WJ, Calvert ML, Tester DJ, Ackerman MJ, Knollmann BC (2016) Circ Arrhythm Electrophysiol 9(8)
    › Primary publication · 27516456 (PubMed) · PMC4988333 (PubMed Central)
  39. Binding of transition metals to S100 proteins. Gilston BA, Skaar EP, Chazin WJ (2016) Sci China Life Sci 59(8): 792-801
    › Primary publication · 27430886 (PubMed) · PMC5123432 (PubMed Central)
  40. Novel calmodulin mutations associated with congenital long QT syndrome affect calcium current in human cardiomyocytes. Pipilas DC, Johnson CN, Webster G, Schlaepfer J, Fellmann F, Sekarski N, Wren LM, Ogorodnik KV, Chazin DM, Chazin WJ, Crotti L, Bhuiyan ZA, George AL (2016) Heart Rhythm 13(10): 2012-9
    › Primary publication · 27374306 (PubMed) · PMC5035189 (PubMed Central)
  41. The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction. Wakeman CA, Moore JL, Noto MJ, Zhang Y, Singleton MD, Prentice BM, Gilston BA, Doster RS, Gaddy JA, Chazin WJ, Caprioli RM, Skaar EP (2016) Nat Commun : 11951
    › Primary publication · 27301800 (PubMed) · PMC4912628 (PubMed Central)
  42. The Response of Acinetobacter baumannii to Zinc Starvation. Nairn BL, Lonergan ZR, Wang J, Braymer JJ, Zhang Y, Calcutt MW, Lisher JP, Gilston BA, Chazin WJ, de Crécy-Lagard V, Giedroc DP, Skaar EP (2016) Cell Host Microbe 19(6): 826-36
    › Primary publication · 27281572 (PubMed) · PMC4901392 (PubMed Central)
  43. Salmonella Mitigates Oxidative Stress and Thrives in the Inflamed Gut by Evading Calprotectin-Mediated Manganese Sequestration. Diaz-Ochoa VE, Lam D, Lee CS, Klaus S, Behnsen J, Liu JZ, Chim N, Nuccio SP, Rathi SG, Mastroianni JR, Edwards RA, Jacobo CM, Cerasi M, Battistoni A, Ouellette AJ, Goulding CW, Chazin WJ, Skaar EP, Raffatellu M (2016) Cell Host Microbe 19(6): 814-25
    › Primary publication · 27281571 (PubMed) · PMC4901528 (PubMed Central)
  44. XPA: A key scaffold for human nucleotide excision repair. Sugitani N, Sivley RM, Perry KE, Capra JA, Chazin WJ (2016) DNA Repair (Amst) : 123-135
    › Primary publication · 27247238 (PubMed) · PMC4958585 (PubMed Central)
  45. Spectrum and Prevalence of CALM1-, CALM2-, and CALM3-Encoded Calmodulin Variants in Long QT Syndrome and Functional Characterization of a Novel Long QT Syndrome-Associated Calmodulin Missense Variant, E141G. Boczek NJ, Gomez-Hurtado N, Ye D, Calvert ML, Tester DJ, Kryshtal D, Hwang HS, Johnson CN, Chazin WJ, Loporcaro CG, Shah M, Papez AL, Lau YR, Kanter R, Knollmann BC, Ackerman MJ (2016) Circ Cardiovasc Genet 9(2): 136-146
    › Primary publication · 26969752 (PubMed) · PMC4907364 (PubMed Central)
  46. Data publication with the structural biology data grid supports live analysis. Meyer PA, Socias S, Key J, Ransey E, Tjon EC, Buschiazzo A, Lei M, Botka C, Withrow J, Neau D, Rajashankar K, Anderson KS, Baxter RH, Blacklow SC, Boggon TJ, Bonvin AM, Borek D, Brett TJ, Caflisch A, Chang CI, Chazin WJ, Corbett KD, Cosgrove MS, Crosson S, Dhe-Paganon S, Di Cera E, Drennan CL, Eck MJ, Eichman BF, Fan QR, Ferré-D'Amaré AR, Fromme JC, Garcia KC, Gaudet R, Gong P, Harrison SC, Heldwein EE, Jia Z, Keenan RJ, Kruse AC, Kvansakul M, McLellan JS, Modis Y, Nam Y, Otwinowski Z, Pai EF, Pereira PJ, Petosa C, Raman CS, Rapoport TA, Roll-Mecak A, Rosen MK, Rudenko G, Schlessinger J, Schwartz TU, Shamoo Y, Sondermann H, Tao YJ, Tolia NH, Tsodikov OV, Westover KD, Wu H, Foster I, Fraser JS, Maia FR, Gonen T, Kirchhausen T, Diederichs K, Crosas M, Sliz P (2016) Nat Commun : 10882
    › Primary publication · 26947396 (PubMed) · PMC4786681 (PubMed Central)
  47. Molecular Basis for the Interaction Between AP4 β4 and its Accessory Protein, Tepsin. Frazier MN, Davies AK, Voehler M, Kendall AK, Borner GH, Chazin WJ, Robinson MS, Jackson LP (2016) Traffic 17(4): 400-15
    › Primary publication · 26756312 (PubMed) · PMC4805503 (PubMed Central)
  48. Identification and Optimization of Anthranilic Acid Based Inhibitors of Replication Protein A. Patrone JD, Pelz NF, Bates BS, Souza-Fagundes EM, Vangamudi B, Camper DV, Kuznetsov AG, Browning CF, Feldkamp MD, Frank AO, Gilston BA, Olejniczak ET, Rossanese OW, Waterson AG, Chazin WJ, Fesik SW (2016) ChemMedChem 11(8): 893-9
    › Primary publication · 26748787 (PubMed) · PMC4838552 (PubMed Central)
  49. The pattern recognition reagents RAGE VC1 and peptide p5 share common binding sites and exhibit specific reactivity with AA amyloid in mice. Kennel SJ, Williams A, Stuckey A, Richey T, Wooliver C, Chazin W, Stern DA, Martin EB, Wall JS (2016) Amyloid 23(1): 8-16
    › Primary publication · 26701064 (PubMed) · PMC4832564 (PubMed Central)
  50. Helicobacter pylori Resists the Antimicrobial Activity of Calprotectin via Lipid A Modification and Associated Biofilm Formation. Gaddy JA, Radin JN, Cullen TW, Chazin WJ, Skaar EP, Trent MS, Algood HM (2015) mBio 6(6): e01349-15
    › Primary publication · 26646009 (PubMed) · PMC4669380 (PubMed Central)
  51. Zinc and Manganese Chelation by Neutrophil S100A8/A9 (Calprotectin) Limits Extracellular Aspergillus fumigatus Hyphal Growth and Corneal Infection. Clark HL, Jhingran A, Sun Y, Vareechon C, de Jesus Carrion S, Skaar EP, Chazin WJ, Calera JA, Hohl TM, Pearlman E (2016) J Immunol 196(1): 336-44
    › Primary publication · 26582948 (PubMed) · PMC4684987 (PubMed Central)
  52. CacyBP/SIP--Structure and variety of functions. Topolska-Woś AM, Chazin WJ, Filipek A (2016) Biochim Biophys Acta 1860(1 Pt A): 79-85
    › Primary publication · 26493724 (PubMed)
  53. Arrhythmogenic Calmodulin Mutations Affect the Activation and Termination of Cardiac Ryanodine Receptor-mediated Ca2+ Release. Søndergaard MT, Tian X, Liu Y, Wang R, Chazin WJ, Chen SR, Overgaard MT (2015) J Biol Chem 290(43): 26151-62
    › Primary publication · 26309258 (PubMed) · PMC4646266 (PubMed Central)
  54. Calprotectin Increases the Activity of the SaeRS Two Component System and Murine Mortality during Staphylococcus aureus Infections. Cho H, Jeong DW, Liu Q, Yeo WS, Vogl T, Skaar EP, Chazin WJ, Bae T (2015) PLoS Pathog 11(7): e1005026
    › Primary publication · 26147796 (PubMed) · PMC4492782 (PubMed Central)
  55. Promotion of BRCA2-Dependent Homologous Recombination by DSS1 via RPA Targeting and DNA Mimicry. Zhao W, Vaithiyalingam S, San Filippo J, Maranon DG, Jimenez-Sainz J, Fontenay GV, Kwon Y, Leung SG, Lu L, Jensen RB, Chazin WJ, Wiese C, Sung P (2015) Mol Cell 59(2): 176-87
    › Primary publication · 26145171 (PubMed) · PMC4506714 (PubMed Central)
  56. Nutritional Immunity: S100 Proteins at the Host-Pathogen Interface. Zackular JP, Chazin WJ, Skaar EP (2015) J Biol Chem 290(31): 18991-8
    › Primary publication · 26055713 (PubMed) · PMC4521021 (PubMed Central)
  57. Biochemical and Proteomic Analysis of Ubiquitination of Hsc70 and Hsp70 by the E3 Ligase CHIP. Soss SE, Rose KL, Hill S, Jouan S, Chazin WJ (2015) PLoS One 10(5): e0128240
    › Primary publication · 26010904 (PubMed) · PMC4444009 (PubMed Central)
  58. Functional dynamics in replication protein A DNA binding and protein recruitment domains. Brosey CA, Soss SE, Brooks S, Yan C, Ivanov I, Dorai K, Chazin WJ (2015) Structure 23(6): 1028-38
    › Primary publication · 26004442 (PubMed) · PMC4456234 (PubMed Central)
  59. The Human Antimicrobial Protein Calgranulin C Participates in Control of Helicobacter pylori Growth and Regulation of Virulence. Haley KP, Delgado AG, Piazuelo MB, Mortensen BL, Correa P, Damo SM, Chazin WJ, Skaar EP, Gaddy JA (2015) Infect Immun 83(7): 2944-56
    › Primary publication · 25964473 (PubMed) · PMC4468547 (PubMed Central)
  60. Zinc regulates a switch between primary and alternative S18 ribosomal proteins in Mycobacterium tuberculosis. Prisic S, Hwang H, Dow A, Barnaby O, Pan TS, Lonzanida JA, Chazin WJ, Steen H, Husson RN (2015) Mol Microbiol 97(2): 263-80
    › Primary publication · 25858183 (PubMed) · PMC4548965 (PubMed Central)
  61. Mechanochemical regulations of RPA's binding to ssDNA. Chen J, Le S, Basu A, Chazin WJ, Yan J (2015) Sci Rep : 9296
    › Primary publication · 25787788 (PubMed) · PMC4365408 (PubMed Central)
  62. Na+ channel function, regulation, structure, trafficking and sequestration. Chen-Izu Y, Shaw RM, Pitt GS, Yarov-Yarovoy V, Sack JT, Abriel H, Aldrich RW, Belardinelli L, Cannell MB, Catterall WA, Chazin WJ, Chiamvimonvat N, Deschenes I, Grandi E, Hund TJ, Izu LT, Maier LS, Maltsev VA, Marionneau C, Mohler PJ, Rajamani S, Rasmusson RL, Sobie EA, Clancy CE, Bers DM (2015) J Physiol 593(6): 1347-60
    › Primary publication · 25772290 (PubMed) · PMC4376415 (PubMed Central)
  63. Simian virus Large T antigen interacts with the N-terminal domain of the 70 kD subunit of Replication Protein A in the same mode as multiple DNA damage response factors. Ning B, Feldkamp MD, Cortez D, Chazin WJ, Friedman KL, Fanning E (2015) PLoS One 10(2): e0116093
    › Primary publication · 25706313 (PubMed) · PMC4337903 (PubMed Central)
  64. Diphenylpyrazoles as replication protein a inhibitors. Waterson AG, Kennedy JP, Patrone JD, Pelz NF, Feldkamp MD, Frank AO, Vangamudi B, Souza-Fagundes EM, Rossanese OW, Chazin WJ, Fesik SW (2015) ACS Med Chem Lett 6(2): 140-5
    › Primary publication · 25699140 (PubMed) · PMC4329576 (PubMed Central)
  65. Dimerization and phosphatase activity of calcyclin-binding protein/Siah-1 interacting protein: the influence of oxidative stress. Topolska-Woś AM, Shell SM, Kilańczyk E, Szczepanowski RH, Chazin WJ, Filipek A (2015) FASEB J 29(5): 1711-24
    › Primary publication · 25609429 (PubMed) · PMC4415008 (PubMed Central)
  66. Human PrimPol is a highly error-prone polymerase regulated by single-stranded DNA binding proteins. Guilliam TA, Jozwiakowski SK, Ehlinger A, Barnes RP, Rudd SG, Bailey LJ, Skehel JM, Eckert KA, Chazin WJ, Doherty AJ (2015) Nucleic Acids Res 43(2): 1056-68
    › Primary publication · 25550423 (PubMed) · PMC4333378 (PubMed Central)
  67. Redefining the DNA-binding domain of human XPA. Sugitani N, Shell SM, Soss SE, Chazin WJ (2014) J Am Chem Soc 136(31): 10830-3
    › Primary publication · 25056193 (PubMed) · PMC4132956 (PubMed Central)
  68. Novel calmodulin mutations associated with congenital arrhythmia susceptibility. Makita N, Yagihara N, Crotti L, Johnson CN, Beckmann BM, Roh MS, Shigemizu D, Lichtner P, Ishikawa T, Aiba T, Homfray T, Behr ER, Klug D, Denjoy I, Mastantuono E, Theisen D, Tsunoda T, Satake W, Toda T, Nakagawa H, Tsuji Y, Tsuchiya T, Yamamoto H, Miyamoto Y, Endo N, Kimura A, Ozaki K, Motomura H, Suda K, Tanaka T, Schwartz PJ, Meitinger T, Kääb S, Guicheney P, Shimizu W, Bhuiyan ZA, Watanabe H, Chazin WJ, George AL (2014) Circ Cardiovasc Genet 7(4): 466-74
    › Primary publication · 24917665 (PubMed) · PMC4140998 (PubMed Central)
  69. Structural analysis of replication protein A recruitment of the DNA damage response protein SMARCAL1. Feldkamp MD, Mason AC, Eichman BF, Chazin WJ (2014) Biochemistry 53(18): 3052-61
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  70. Discovery of a potent stapled helix peptide that binds to the 70N domain of replication protein A. Frank AO, Vangamudi B, Feldkamp MD, Souza-Fagundes EM, Luzwick JW, Cortez D, Olejniczak ET, Waterson AG, Rossanese OW, Chazin WJ, Fesik SW (2014) J Med Chem 57(6): 2455-61
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  71. Insights into eukaryotic primer synthesis from structures of the p48 subunit of human DNA primase. Vaithiyalingam S, Arnett DR, Aggarwal A, Eichman BF, Fanning E, Chazin WJ (2014) J Mol Biol 426(3): 558-69
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  72. Discovery of a potent inhibitor of replication protein a protein-protein interactions using a fragment-linking approach. Frank AO, Feldkamp MD, Kennedy JP, Waterson AG, Pelz NF, Patrone JD, Vangamudi B, Camper DV, Rossanese OW, Chazin WJ, Fesik SW (2013) J Med Chem 56(22): 9242-50
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  73. Xeroderma pigmentosum complementation group C protein (XPC) serves as a general sensor of damaged DNA. Shell SM, Hawkins EK, Tsai MS, Hlaing AS, Rizzo CJ, Chazin WJ (2013) DNA Repair (Amst) 12(11): 947-53
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  74. Surface reengineering of RPA70N enables cocrystallization with an inhibitor of the replication protein A interaction motif of ATR interacting protein. Feldkamp MD, Frank AO, Kennedy JP, Patrone JD, Vangamudi B, Waterson AG, Fesik SW, Chazin WJ (2013) Biochemistry 52(37): 6515-24
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  75. Discovery of Protein-Protein Interaction Inhibitors of Replication Protein A. Patrone JD, Kennedy JP, Frank AO, Feldkamp MD, Vangamudi B, Pelz NF, Rossanese OW, Waterson AG, Chazin WJ, Fesik SW (2013) ACS Med Chem Lett 4(7): 601-605
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  76. Stable RAGE-heparan sulfate complexes are essential for signal transduction. Xu D, Young JH, Krahn JM, Song D, Corbett KD, Chazin WJ, Pedersen LC, Esko JD (2013) ACS Chem Biol 8(7): 1611-20
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  77. Activation of UbcH5c~Ub is the result of a shift in interdomain motions of the conjugate bound to U-box E3 ligase E4B. Soss SE, Klevit RE, Chazin WJ (2013) Biochemistry 52(17): 2991-9
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  78. Calmodulin mutations associated with recurrent cardiac arrest in infants. Crotti L, Johnson CN, Graf E, De Ferrari GM, Cuneo BF, Ovadia M, Papagiannis J, Feldkamp MD, Rathi SG, Kunic JD, Pedrazzini M, Wieland T, Lichtner P, Beckmann BM, Clark T, Shaffer C, Benson DW, Kääb S, Meitinger T, Strom TM, Chazin WJ, Schwartz PJ, George AL (2013) Circulation 127(9): 1009-17
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  79. A new structural framework for integrating replication protein A into DNA processing machinery. Brosey CA, Yan C, Tsutakawa SE, Heller WT, Rambo RP, Tainer JA, Ivanov I, Chazin WJ (2013) Nucleic Acids Res 41(4): 2313-27
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  80. Lysophosphatidic acid targets vascular and oncogenic pathways via RAGE signaling. Rai V, Touré F, Chitayat S, Pei R, Song F, Li Q, Zhang J, Rosario R, Ramasamy R, Chazin WJ, Schmidt AM (2012) J Exp Med 209(13): 2339-50
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  81. Sample preparation methods to analyze DNA-induced structural changes in replication protein A. Brosey CA, Tsutakawa SE, Chazin WJ (2012) Methods Mol Biol : 101-22
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  82. Structure of an E3:E2~Ub complex reveals an allosteric mechanism shared among RING/U-box ligases. Pruneda JN, Littlefield PJ, Soss SE, Nordquist KA, Chazin WJ, Brzovic PS, Klevit RE (2012) Mol Cell 47(6): 933-42
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  83. Zinc sequestration by the neutrophil protein calprotectin enhances Salmonella growth in the inflamed gut. Liu JZ, Jellbauer S, Poe AJ, Ton V, Pesciaroli M, Kehl-Fie TE, Restrepo NA, Hosking MP, Edwards RA, Battistoni A, Pasquali P, Lane TE, Chazin WJ, Vogl T, Roth J, Skaar EP, Raffatellu M (2012) Cell Host Microbe 11(3): 227-39
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  84. Binding to retinoblastoma pocket domain does not alter the inter-domain flexibility of the J domain of SV40 large T antigen. Williams CK, Vaithiyalingam S, Hammel M, Pipas J, Chazin WJ (2012) Arch Biochem Biophys 518(2): 111-8
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  85. A high-throughput fluorescence polarization anisotropy assay for the 70N domain of replication protein A. Souza-Fagundes EM, Frank AO, Feldkamp MD, Dorset DC, Chazin WJ, Rossanese OW, Olejniczak ET, Fesik SW (2012) Anal Biochem 421(2): 742-9
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  86. Human DNA helicase B (HDHB) binds to replication protein A and facilitates cellular recovery from replication stress. Guler GD, Liu H, Vaithiyalingam S, Arnett DR, Kremmer E, Chazin WJ, Fanning E (2012) J Biol Chem 287(9): 6469-81
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  87. BID binds to replication protein A and stimulates ATR function following replicative stress. Liu Y, Vaithiyalingam S, Shi Q, Chazin WJ, Zinkel SS (2011) Mol Cell Biol 31(21): 4298-309
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  88. E2 conjugating enzyme selectivity and requirements for function of the E3 ubiquitin ligase CHIP. Soss SE, Yue Y, Dhe-Paganon S, Chazin WJ (2011) J Biol Chem 286(24): 21277-86
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  89. Structural basis for ligand recognition and activation of RAGE. Koch M, Chitayat S, Dattilo BM, Schiefner A, Diez J, Chazin WJ, Fritz G (2010) Structure 18(10): 1342-52
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  90. Insights into eukaryotic DNA priming from the structure and functional interactions of the 4Fe-4S cluster domain of human DNA primase. Vaithiyalingam S, Warren EM, Eichman BF, Chazin WJ (2010) Proc Natl Acad Sci U S A 107(31): 13684-9
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  91. Solution NMR structure of the C-terminal DNA binding domain of Mcm10 reveals a conserved MCM motif. Robertson PD, Chagot B, Chazin WJ, Eichman BF (2010) J Biol Chem 285(30): 22942-9
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  92. Structure of a DNA polymerase alpha-primase domain that docks on the SV40 helicase and activates the viral primosome. Huang H, Weiner BE, Zhang H, Fuller BE, Gao Y, Wile BM, Zhao K, Arnett DR, Chazin WJ, Fanning E (2010) J Biol Chem 285(22): 17112-22
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  93. Structural dynamics and single-stranded DNA binding activity of the three N-terminal domains of the large subunit of replication protein A from small angle X-ray scattering. Pretto DI, Tsutakawa S, Brosey CA, Castillo A, Chagot ME, Smith JA, Tainer JA, Chazin WJ (2010) Biochemistry 49(13): 2880-9
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  94. Direct ubiquitination of beta-catenin by Siah-1 and regulation by the exchange factor TBL1. Dimitrova YN, Li J, Lee YT, Rios-Esteves J, Friedman DB, Choi HJ, Weis WI, Wang CY, Chazin WJ (2010) J Biol Chem 285(18): 13507-16
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  95. S100A8/A9 induces autophagy and apoptosis via ROS-mediated cross-talk between mitochondria and lysosomes that involves BNIP3. Ghavami S, Eshragi M, Ande SR, Chazin WJ, Klonisch T, Halayko AJ, McNeill KD, Hashemi M, Kerkhoff C, Los M (2010) Cell Res 20(3): 314-31
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  96. S100A8/A9: a Janus-faced molecule in cancer therapy and tumorgenesis. Ghavami S, Chitayat S, Hashemi M, Eshraghi M, Chazin WJ, Halayko AJ, Kerkhoff C (2009) Eur J Pharmacol 625(1-3): 73-83
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  97. Replication protein A stimulates the Werner syndrome protein branch migration activity. Sowd G, Wang H, Pretto D, Chazin WJ, Opresko PL (2009) J Biol Chem 284(50): 34682-91
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  98. Engineering a ubiquitin ligase reveals conformational flexibility required for ubiquitin transfer. Qian SB, Waldron L, Choudhary N, Klevit RE, Chazin WJ, Patterson C (2009) J Biol Chem 284(39): 26797-802
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  99. Physical interactions between Mcm10, DNA, and DNA polymerase alpha. Warren EM, Huang H, Fanning E, Chazin WJ, Eichman BF (2009) J Biol Chem 284(36): 24662-72
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  100. Checkpoint kinase ATR promotes nucleotide excision repair of UV-induced DNA damage via physical interaction with xeroderma pigmentosum group A. Shell SM, Li Z, Shkriabai N, Kvaratskhelia M, Brosey C, Serrano MA, Chazin WJ, Musich PR, Zou Y (2009) J Biol Chem 284(36): 24213-22
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  101. Structural basis for DNA binding by replication initiator Mcm10. Warren EM, Vaithiyalingam S, Haworth J, Greer B, Bielinsky AK, Chazin WJ, Eichman BF (2008) Structure 16(12): 1892-901
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  102. Subversion of antimicrobial calprotectin (S100A8/S100A9 complex) in the cytoplasm of TR146 epithelial cells after invasion by Listeria monocytogenes. Zaia AA, Sappington KJ, Nisapakultorn K, Chazin WJ, Dietrich EA, Ross KF, Herzberg MC (2009) Mucosal Immunol 2(1): 43-53
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  103. The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling. Xu X, Vaithiyalingam S, Glick GG, Mordes DA, Chazin WJ, Cortez D (2008) Mol Cell Biol 28(24): 7345-53
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  104. Structure of the S100A6 complex with a fragment from the C-terminal domain of Siah-1 interacting protein: a novel mode for S100 protein target recognition. Lee YT, Dimitrova YN, Schneider G, Ridenour WB, Bhattacharya S, Soss SE, Caprioli RM, Filipek A, Chazin WJ (2008) Biochemistry 47(41): 10921-32
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  105. S100A8/A9 at low concentration promotes tumor cell growth via RAGE ligation and MAP kinase-dependent pathway. Ghavami S, Rashedi I, Dattilo BM, Eshraghi M, Chazin WJ, Hashemi M, Wesselborg S, Kerkhoff C, Los M (2008) J Leukoc Biol 83(6): 1484-92
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  106. S100A8/9 induces cell death via a novel, RAGE-independent pathway that involves selective release of Smac/DIABLO and Omi/HtrA2. Ghavami S, Kerkhoff C, Chazin WJ, Kadkhoda K, Xiao W, Zuse A, Hashemi M, Eshraghi M, Schulze-Osthoff K, Klonisch T, Los M (2008) Biochim Biophys Acta 1783(2): 297-311
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