Anne Kenworthy
Last active: 2/12/2016

Profile

One of the major goals of my research program is to understand the role of membrane microdomains such as lipid rafts and caveolae in health and disease. We address this question using a combination of live cell imaging, cell biology, biophysics, and biochemical approaches. Current projects ongoing in the lab include:

1. How do proteins and lipid cooperate to build functional raft domains?

2. How do bacterial toxins exploit lipid rafts as a mechanism to enter host cells?

3. How do cholesterol and lipid rafts contribute to the progression of Alzheimer's disease?

4. How do mutations in caveolin-1 and defects in caveolae cause human diseases such as breast cancer and pulmonary arterial hypertension?

We are also interested in diffusion, a fundamental process that underlies all cellular functions. We are working in collaboration with biomathematicians to develop widely accessible methods to calibrate, measure and quantify protein and lipid diffusion in living cells. We are also applying these approaches to study novel protein complexes in the autophagy pathway, a housekeeping mechanism used by cells to maintain homeostasis in times of starvation and stress.

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Topologically Diverse Human Membrane Proteins Partition to Liquid-Disordered Domains in Phase-Separated Lipid Vesicles. Schlebach JP, Barrett PJ, Day CA, Kim JH, Kenworthy AK, Sanders CR (2016) Biochemistry 55(7): 985-8
    › Primary publication · 26859249 (PubMed) · PMC4766968 (PubMed Central)
  2. Nuclear LC3 Associates with Slowly Diffusing Complexes that Survey the Nucleolus. Kraft LJ, Manral P, Dowler J, Kenworthy AK (2016) Traffic 17(4): 369-99
    › Primary publication · 26728248 (PubMed) · PMC4975375 (PubMed Central)
  3. Validation of Normalizations, Scaling, and Photofading Corrections for FRAP Data Analysis. Kang M, Andreani M, Kenworthy AK (2015) PLoS One 10(5): e0127966
    › Primary publication · 26017223 (PubMed) · PMC4446327 (PubMed Central)
  4. Lipid Domains. Preface. Kenworthy AK (2015) Curr Top Membr : xiii-xvii
    › Primary publication · 26015288 (PubMed) · PMC4984669 (PubMed Central)
  5. Microtubule motors power plasma membrane tubulation in clathrin-independent endocytosis. Day CA, Baetz NW, Copeland CA, Kraft LJ, Han B, Tiwari A, Drake KR, De Luca H, Chinnapen DJ, Davidson MW, Holmes RK, Jobling MG, Schroer TA, Lencer WI, Kenworthy AK (2015) Traffic 16(6): 572-90
    › Primary publication · 25690058 (PubMed) · PMC4440230 (PubMed Central)
  6. Functions of cholera toxin B-subunit as a raft cross-linker. Day CA, Kenworthy AK (2015) Essays Biochem : 135-45
    › Primary publication · 25658350 (PubMed) · PMC4346142 (PubMed Central)
  7. Tagging strategies strongly affect the fate of overexpressed caveolin-1. Han B, Tiwari A, Kenworthy AK (2015) Traffic 16(4): 417-38
    › Primary publication · 25639341 (PubMed) · PMC4440517 (PubMed Central)
  8. Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis. Renard HF, Simunovic M, Lemière J, Boucrot E, Garcia-Castillo MD, Arumugam S, Chambon V, Lamaze C, Wunder C, Kenworthy AK, Schmidt AA, McMahon HT, Sykes C, Bassereau P, Johannes L (2015) Nature 517(7535): 493-6
    › Primary publication · 25517096 (PubMed) · PMC4342003 (PubMed Central)
  9. Heterozygous null bone morphogenetic protein receptor type 2 mutations promote SRC kinase-dependent caveolar trafficking defects and endothelial dysfunction in pulmonary arterial hypertension. Prewitt AR, Ghose S, Frump AL, Datta A, Austin ED, Kenworthy AK, de Caestecker MP (2015) J Biol Chem 290(2): 960-71
    › Primary publication · 25411245 (PubMed) · PMC4294523 (PubMed Central)
  10. Size, stoichiometry, and organization of soluble LC3-associated complexes. Kraft LJ, Nguyen TA, Vogel SS, Kenworthy AK (2014) Autophagy 10(5): 861-77
    › Primary publication · 24646892 (PubMed) · PMC4768459 (PubMed Central)
  11. Motor and tail homology 1 (Th1) domains antagonistically control myosin-1 dynamics. Mazerik JN, Kraft LJ, Kenworthy AK, Tyska MJ (2014) Biophys J 106(3): 649-58
    › Primary publication · 24507605 (PubMed) · PMC3944834 (PubMed Central)
  12. Cholesterol as a co-solvent and a ligand for membrane proteins. Song Y, Kenworthy AK, Sanders CR (2014) Protein Sci 23(1): 1-22
    › Primary publication · 24155031 (PubMed) · PMC3892294 (PubMed Central)
  13. Proposed correction to Feder's anomalous diffusion FRAP equations. Kang M, DiBenedetto E, Kenworthy AK (2011) Biophys J 100(3): 791-792
    › Primary publication · 21281595 (PubMed) · PMC3030171 (PubMed Central)
  14. NHE3 mobility in brush borders increases upon NHERF2-dependent stimulation by lyophosphatidic acid. Cha B, Zhu XC, Chen W, Jones M, Ryoo S, Zachos NC, Chen TE, Lin R, Sarker R, Kenworthy AK, Tse M, Kovbasnjuk O, Donowitz M (2010) J Cell Sci 123(Pt 14): 2434-43
    › Primary publication · 20571054 (PubMed) · PMC2936692 (PubMed Central)
  15. On the use of Ripley's K-function and its derivatives to analyze domain size. Kiskowski MA, Hancock JF, Kenworthy AK (2009) Biophys J 97(4): 1095-103
    › Primary publication · 19686657 (PubMed) · PMC2726315 (PubMed Central)
  16. The lateral mobility of NHE3 on the apical membrane of renal epithelial OK cells is limited by the PDZ domain proteins NHERF1/2, but is dependent on an intact actin cytoskeleton as determined by FRAP. Cha B, Kenworthy A, Murtazina R, Donowitz M (2004) J Cell Sci 117(Pt 15): 3353-65
    › Primary publication · 15226406 (PubMed)