Jeffrey Davidson
Last active: 2/19/2015

Profile

Elastin is a highly insoluble matrix protein with very low turnover. We study elastin gene expression in several biological systems. cDNA and antibody probes are used, respectively, to study regulation of elastin mRNA and protein synthesis, particularly related to modulation of elastin metabolism in diseases of human connective tissues such as blood vessels, lung and skin. Transcriptional and post-transcriptional regulation of matrix synthesis is evident in this system The role of mRNA stability, cis-acting mutations, and trans-acting factors are being evaluated in the context of elastic tissue diseases and growth factor responses. Cytokine effects are tested on cells from vascular smooth muscle, skin, and vocal fold. Several human diseases involve loss or excessive accumulation of elastic tissue, including an unusual aging syndrome, Hutchinson-Gilford progeria, and recessive and acquired forms of cutis laxa.. The consequences of elastin mutations that we discovered in cutis laxa is under intense examination

Connective tissue production and remodeling are also critical to proper wound repair. We have been examining the mechanisms of collagen and elastin turnover in pulmonary fibrosis and related diseases. We also use animal models to explore how addition or local inactivation of growth factors such as basic fibroblast growth factor, keratinocyte growth factor, transforming growth factor-? superfamily members, platelet derived growth factor and connective tissue growth affect the process and outcome of wound healing. Defective wound healing is an important clinical problem that is under study in fibrotic, aging and diabetic animal populations. Cellular and tissue-equivalent models are used where they appear to accurately reflect tissue behavior. Evaluation of gene expression by in situ and filter hybridization reveals diverse mechanisms, including the influence of metalloproteinases involved in reorganization of fibrous connective tissue.

We have used a novel form of gene therapy to express various factors in wounds with clear effects on the rate of healing. This technique offers the possibility of manipulating the healing process in a new and precise way, since recombinant expression vectors can be readily modified to understand structure-function relationships in an authentic biological context. Gene therapy of wounds also offers a rapid way to screen for wound healing functions of newly discovered cDNAs.

The laboratory is also part of the Vanderbilt Free-Electron Laser Center. This is a multidisciplinary program in which this laboratory is examining how tissues respond and heal after irradiation from the most powerful source of pulsed, infrared light energy in the country. .

Publications

The following timeline graph is generated from all co-authored publications.

  1. Wound samples: moving towards a standardised method of collection and analysis. Ramsay S, Cowan L, Davidson JM, Nanney L, Schultz G (2016) Int Wound J 13(5): 880-91
    › Primary publication · 25581688 (PubMed)
  2. Molecular imaging-assisted optimization of hsp70 expression during laser-induced thermal preconditioning for wound repair enhancement. Wilmink GJ, Opalenik SR, Beckham JT, Abraham AA, Nanney LB, Mahadevan-Jansen A, Davidson JM, Jansen ED (2009) J Invest Dermatol 129(1): 205-16
    › Primary publication · 18580963 (PubMed) · PMC3846389 (PubMed Central)
  3. Injectable biodegradable polyurethane scaffolds with release of platelet-derived growth factor for tissue repair and regeneration. Hafeman AE, Li B, Yoshii T, Zienkiewicz K, Davidson JM, Guelcher SA (2008) Pharm Res 25(10): 2387-99
    › Primary publication · 18516665 (PubMed) · PMC3842433 (PubMed Central)
  4. First-class delivery: getting growth factors to their destination. Davidson JM (2008) J Invest Dermatol 128(6): 1360-2
    › Primary publication · 18478013 (PubMed)
  5. Tissue profiling MALDI mass spectrometry reveals prominent calcium-binding proteins in the proteome of regenerative MRL mouse wounds. Caldwell RL, Opalenik SR, Davidson JM, Caprioli RM, Nanney LB (2008) Wound Repair Regen 16(3): 442-9
    › Primary publication · 18282264 (PubMed) · PMC2891803 (PubMed Central)
  6. Nanovehicular intracellular delivery systems. Prokop A, Davidson JM (2008) J Pharm Sci 97(9): 3518-90
    › Primary publication · 18200527 (PubMed) · PMC3747665 (PubMed Central)
  7. Mechanical abrasion improves early incorporation of small intestinal submucosa. Rauth TP, Poulose BK, Davidson JM, Nanney LB, Holzman MD (2007) Am Surg 73(7): 647-51; discussion 651
    › Primary publication · 17674934 (PubMed)
  8. Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells. Hartig SM, Greene RR, Carlesso G, Higginbotham JN, Khan WN, Prokop A, Davidson JM (2007) Biomaterials 28(26): 3843-55
    › Primary publication · 17560645 (PubMed) · PMC2000344 (PubMed Central)
  9. Canine subglottic stenosis as a model for excessive fibrosis: a pilot histologic and immunohistochemical analysis. Charous SJ, Ossoff RH, Reinisch L, Davidson JM (1996) Wound Repair Regen 4(4): 444-53
    › Primary publication · 17309695 (PubMed)
  10. Development of improved nanoparticulate polyelectrolyte complex physicochemistry by nonstoichiometric mixing of polyions with similar molecular weights. Hartig SM, Carlesso G, Davidson JM, Prokop A (2007) Biomacromolecules 8(1): 265-72
    › Primary publication · 17206816 (PubMed)