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Jonathan Irish is Assistant Professor at Vanderbilt University in the Departments of Cell & Developmental Biology and Pathology, Microbiology & Immunology | Google scholar

My Ph.D. training was in cancer biology, immunology, and computational biology and my undergraduate training was in chemistry and biology.  My lab’s expertise is in analysis of high-dimensional single cell data from human tissue using approaches I created during my PhD training.  I am also the co-founder of Cytobank Inc., a cloud analytics platform for single cell data used by researchers around the world and leading pharmaceutical companies.  

Irish lab website (with protocols & lab member information): http://my.vanderbilt.edu/irishlab/

Publications

The following timeline graph is generated from all co-authored publications.

Featured publications are shown below:

  1. Computational immune monitoring reveals abnormal double negative T cells present across human tumor types. Greenplate AR, McClanahan DD, Oberholtzer BK, Doxie DB, Roe CE, Diggins KE, Leelatian N, Rasmussen ML, Kelley MC, Gama V, Siska PJ, Rathmell JC, Ferrell PB, Johnson DB, Irish JM (2018) Cancer Immunol Res
    › Primary publication · 30413431 (PubMed)
  2. Machine learning reveals chronic graft-versus-host disease phenotypes and stratifies survival after stem cell transplant for hematologic malignancies. Gandelman JS, Byrne MT, Mistry AM, Polikowsky HG, Diggins KE, Chen H, Lee SJ, Arora M, Cutler C, Flowers M, Pidala J, Irish JM, Jagasia MH (2018) Haematologica
    › Primary publication · 30237265 (PubMed)
  3. BRAF and MEK inhibitor therapy eliminates nestin expressing melanoma cells in human tumors. Doxie DB, Greenplate AR, Gandelman JS, Diggins KE, Roe CE, Dahlman KB, Sosman JA, Kelley MC, Irish JM (2018) Pigment Cell Melanoma Res
    › Primary publication · 29778085 (PubMed)
  4. Generating Quantitative Cell Identity Labels with Marker Enrichment Modeling (MEM). Diggins KE, Gandelman JS, Roe CE, Irish JM (2018) Curr Protoc Cytom : 10.21.1-10.21.28
    › Primary publication · 29345329 (PubMed) · PMC5774653 (PubMed Central)
  5. Discovery of human cell selective effector molecules using single cell multiplexed activity metabolomics. Earl DC, Ferrell PB, Leelatian N, Froese JT, Reisman BJ, Irish JM, Bachmann BO (2018) Nat Commun 9(1): 39
    › Primary publication · 29295987 (PubMed) · PMC5750220 (PubMed Central)
  6. Mass cytometry deep phenotyping of human mononuclear phagocytes and myeloid-derived suppressor cells from human blood and bone marrow. Roussel M, Ferrell PB, Greenplate AR, Lhomme F, Le Gallou S, Diggins KE, Johnson DB, Irish JM (2017) J Leukoc Biol 102(2): 437-447
    › Primary publication · 28400539 (PubMed)
  7. Preparing Viable Single Cells from Human Tissue and Tumors for Cytomic Analysis. Leelatian N, Doxie DB, Greenplate AR, Sinnaeve J, Ihrie RA, Irish JM (2017) Curr Protoc Mol Biol : 25C.1.1-25C.1.23
    › Primary publication · 28369679 (PubMed) · PMC5518778 (PubMed Central)
  8. Characterizing cell subsets using marker enrichment modeling. Diggins KE, Greenplate AR, Leelatian N, Wogsland CE, Irish JM (2017) Nat Methods 14(3): 275-278
    › Primary publication · 28135256 (PubMed) · PMC5330853 (PubMed Central)
  9. Mass Cytometry of Follicular Lymphoma Tumors Reveals Intrinsic Heterogeneity in Proteins Including HLA-DR and a Deficit in Nonmalignant Plasmablast and Germinal Center B-Cell Populations. Wogsland CE, Greenplate AR, Kolstad A, Myklebust JH, Irish JM, Huse K (2017) Cytometry B Clin Cytom 92(1): 79-87
    › Primary publication · 27933753 (PubMed) · PMC5506779 (PubMed Central)
  10. Single cell analysis of human tissues and solid tumors with mass cytometry. Leelatian N, Doxie DB, Greenplate AR, Mobley BC, Lehman JM, Sinnaeve J, Kauffmann RM, Werkhaven JA, Mistry AM, Weaver KD, Thompson RC, Massion PP, Hooks MA, Kelley MC, Chambless LB, Ihrie RA, Irish JM (2017) Cytometry B Clin Cytom 92(1): 68-78
    › Primary publication · 27598832 (PubMed) · PMC5459378 (PubMed Central)
  11. Systems immune monitoring in cancer therapy. Greenplate AR, Johnson DB, Ferrell PB, Irish JM (2016) Eur J Cancer : 77-84
    › Primary publication · 27155446 (PubMed) · PMC4885747 (PubMed Central)
  12. High-Dimensional Analysis of Acute Myeloid Leukemia Reveals Phenotypic Changes in Persistent Cells during Induction Therapy. Ferrell PB, Diggins KE, Polikowsky HG, Mohan SR, Seegmiller AC, Irish JM (2016) PLoS One 11(4): e0153207
    › Primary publication · 27074138 (PubMed) · PMC4830605 (PubMed Central)
  13. Myelodysplastic Syndrome Revealed by Systems Immunology in a Melanoma Patient Undergoing Anti-PD-1 Therapy. Greenplate AR, Johnson DB, Roussel M, Savona MR, Sosman JA, Puzanov I, Ferrell PB, Irish JM (2016) Cancer Immunol Res 4(6): 474-480
    › Primary publication · 26966176 (PubMed) · PMC4891280 (PubMed Central)
  14. Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry. Leelatian N, Diggins KE, Irish JM (2015) Methods Mol Biol : 99-113
    › Primary publication · 26542718 (PubMed) · PMC4656023 (PubMed Central)
  15. Cutting Edge: Redox Signaling Hypersensitivity Distinguishes Human Germinal Center B Cells. Polikowsky HG, Wogsland CE, Diggins KE, Huse K, Irish JM (2015) J Immunol 195(4): 1364-1367
    › Primary publication · 26157177 (PubMed) · PMC4530023 (PubMed Central)
  16. Methods for discovery and characterization of cell subsets in high dimensional mass cytometry data. Diggins KE, Ferrell PB, Irish JM (2015) Methods : 55-63
    › Primary publication · 25979346 (PubMed) · PMC4468028 (PubMed Central)
  17. Beyond the age of cellular discovery. Irish JM (2014) Nat Immunol 15(12): 1095-7
    › Primary publication · 25396342 (PubMed)
  18. High-dimensional single-cell cancer biology. Irish JM, Doxie DB (2014) Curr Top Microbiol Immunol : 1-21
    › Primary publication · 24671264 (PubMed) · PMC4216808 (PubMed Central)
  19. High PD-1 expression and suppressed cytokine signaling distinguish T cells infiltrating follicular lymphoma tumors from peripheral T cells. Myklebust JH, Irish JM, Brody J, Czerwinski DK, Houot R, Kohrt HE, Timmerman J, Said J, Green MR, Delabie J, Kolstad A, Alizadeh AA, Levy R (2013) Blood 121(8): 1367-76
    › Primary publication · 23297127 (PubMed) · PMC3578953 (PubMed Central)
  20. Hierarchy in somatic mutations arising during genomic evolution and progression of follicular lymphoma. Green MR, Gentles AJ, Nair RV, Irish JM, Kihira S, Liu CL, Kela I, Hopmans ES, Myklebust JH, Ji H, Plevritis SK, Levy R, Alizadeh AA (2013) Blood 121(9): 1604-11
    › Primary publication · 23297126 (PubMed) · PMC3587323 (PubMed Central)
  21. Web-based analysis and publication of flow cytometry experiments. Kotecha N, Krutzik PO, Irish JM (2010) Curr Protoc Cytom : Unit10.17
    › Primary publication · 20578106 (PubMed) · PMC4208272 (PubMed Central)
  22. B-cell signaling networks reveal a negative prognostic human lymphoma cell subset that emerges during tumor progression. Irish JM, Myklebust JH, Alizadeh AA, Houot R, Sharman JP, Czerwinski DK, Nolan GP, Levy R (2010) Proc Natl Acad Sci U S A 107(29): 12747-54
    › Primary publication · 20543139 (PubMed) · PMC2919949 (PubMed Central)
  23. Kinetics of B cell receptor signaling in human B cell subsets mapped by phosphospecific flow cytometry. Irish JM, Czerwinski DK, Nolan GP, Levy R (2006) J Immunol 177(3): 1581-9
    › Primary publication · 16849466 (PubMed)
  24. Altered B-cell receptor signaling kinetics distinguish human follicular lymphoma B cells from tumor-infiltrating nonmalignant B cells. Irish JM, Czerwinski DK, Nolan GP, Levy R (2006) Blood 108(9): 3135-42
    › Primary publication · 16835385 (PubMed) · PMC1895530 (PubMed Central)
  25. Mapping normal and cancer cell signalling networks: towards single-cell proteomics. Irish JM, Kotecha N, Nolan GP (2006) Nat Rev Cancer 6(2): 146-55
    › Primary publication · 16491074 (PubMed)
  26. Single cell profiling of potentiated phospho-protein networks in cancer cells. Irish JM, Hovland R, Krutzik PO, Perez OD, Bruserud Ø, Gjertsen BT, Nolan GP (2004) Cell 118(2): 217-28
    › Primary publication · 15260991 (PubMed)