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Resource Type Keywords  ID
BAC maxiprep using Nucleobond BAC 100 columnsProtocolBAC, BAC DNA prep1441
BAC recombination protocols (Mortlock lab 2012)ProtocolBAC, electroporation, Recombination3165
BAC subcloning by gap repairProtocolBAC, recombination, subcloning, targeting vector3166
Quantitation of transgene copy numberProtocolcopy number505
2017-2018 mouse genome editing projects: Improved outcomesPageCRISPR/Cas9, CRISPR/Cas9 knockout, CRISPR/Cas9 knockin, CRISPR15984
Genome EditingServiceCrispr, Cas9, Microinjection11363
Guidelines for preparing CRISPR/Cas9-expressing DNA plasmids for CRISPR injections (10/21/14)ProtocolCRISPR, DNA isolation10724
Crispr Biology: From Foundational to Translational SciencePageCRISPR, Lecture12092
Precipitation of long ssDNA oligos to remove potential contaminants before CRISPR injectionsProtocolcrispr, oligo13328
Cloning protospacer adapters into PX plasmids via BbsI siteProtocolCRISPR, PX330, PX335, PX458, PX45910702
Advanced cryopreservation and rederivation techniquesPagecryopreservation, in vitro fertilization, rederivation, IVF16130
Vanderbilt Cryopreserved Mouse RepositoryPageCryopreserved, VCMR, Repository, Mouse, Cryopreservation15874
BAC DNA CsCl gradient purificationProtocolCsCl purification, BAC, BAC DNA prep1121
Mouse NomenclaturePageFormal Name, guide for nomenclature, MGI, Mouse Genome Informatics, nomenclature14588
The TMESCSR now offers gene-editing project design, founder screening, and founder analysis. Contact us for a consultation and estimate.Pagefounder analysis, project design, founder screening15219
pMCS.DT-APlasmid Vectorgene targeting, DT, diptheria toxin19
Generic mouse genotyping protocolPagegenotyping CRISPR15913
Thawing embryosProtocolJAX slow freeze, cryo straws, cryo vials, 8 cell mouse embryos421
KOMP and EUCOMM Guidelines and ResourcesProtocolKOMP, JM8, JM8A, EUCOMM1438
Preparation of mouse ES cells for karyotypingProtocolmESC, karyotyping297
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