Staphylococcus aureus stimulates neutrophil recruitment by stimulating interleukin-8 production in dog trachea.

Massion PP, H├ębert CA, Leong S, Chan B, Inoue H, Grattan K, Sheppard D, Nadel JA
Am J Physiol. 1995 268 (1 Pt 1): L85-94

PMID: 7840233 · DOI:10.1152/ajplung.1995.268.1.L85

The present study examined whether neutrophil recruitment in dog airways by Staphylococcus aureus is mediated by interleukin-8 (IL-8). S. aureus culture supernatant was superfused into an isolated tracheal segment in six dogs, and neutrophil recruitment and IL-8 concentrations were measured in the superfusate. Dog IL-8 was cloned, expressed in Escherichia coli, purified by chromatography, and shown to be biologically active. With the use of an enzyme-linked immunoabsorbent assay (ELISA) for the measurement of dog IL-8, we showed that S. aureus supernatant induced neutrophil recruitment and increased IL-8 concentration in the superfusate in a time-dependent manner. The chemotactic activity present in the superfusate 6 h after superfusion with S. aureus was inhibited by an anti-IL-8 antibody. S. aureus supernatant also stimulated IL-8 production and gene expression by cultured canine tracheal epithelial cells. These results provide evidence that IL-8 plays a major role in S. aureus-induced neutrophil recruitment in the airways by stimulating IL-8 production in airway cells.

MeSH Terms (21)

Amino Acid Sequence Animals Base Sequence Chemotactic Factors Chemotaxis, Leukocyte Cloning, Molecular Dogs Epithelial Cells Epithelium Humans Interleukin-8 Molecular Probes Molecular Sequence Data Neutrophils Osmolar Concentration Rabbits Recombinant Proteins Sheep Staphylococcus aureus Time Factors Trachea

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