Human DNA polymerase η accommodates RNA for strand extension.

Su Y, Egli M, Guengerich FP
J Biol Chem. 2017 292 (44): 18044-18051

PMID: 28972162 · PMCID: PMC5672030 · DOI:10.1074/jbc.M117.809723

Ribonucleotides are the natural analogs of deoxyribonucleotides, which can be misinserted by DNA polymerases, leading to the most abundant DNA lesions in genomes. During replication, DNA polymerases tolerate patches of ribonucleotides on the parental strands to different extents. The majority of human DNA polymerases have been reported to misinsert ribonucleotides into genomes. However, only PrimPol, DNA polymerase α, telomerase, and the mitochondrial human DNA polymerase (hpol) γ have been shown to tolerate an entire RNA strand. Y-family hpol η is known for translesion synthesis opposite the UV-induced DNA lesion cyclobutane pyrimidine dimer and was recently found to incorporate ribonucleotides into DNA. Here, we report that hpol η is able to bind DNA/DNA, RNA/DNA, and DNA/RNA duplexes with similar affinities. In addition, hpol η, as well as another Y-family DNA polymerase, hpol κ, accommodates RNA as one of the two strands during primer extension, mainly by inserting dNMPs opposite unmodified templates or DNA lesions, such as 8-oxo-2'-deoxyguanosine or cyclobutane pyrimidine dimer, even in the presence of an equal amount of the DNA/DNA substrate. The discovery of this RNA-accommodating ability of hpol η redefines the traditional concept of human DNA polymerases and indicates potential new functions of hpol η .

© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

MeSH Terms (19)

8-Hydroxy-2'-Deoxyguanosine Base Pair Mismatch Deoxyguanosine DNA-Directed DNA Polymerase DNA Primers DNA Replication Electrophoretic Mobility Shift Assay Humans Kinetics Nucleic Acid Heteroduplexes Nucleic Acid Hybridization Oligodeoxyribonucleotides Oligoribonucleotides Pyrimidine Dimers Recombinant Proteins Reverse Transcription RNA Substrate Specificity Transcription Elongation, Genetic

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