Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches.

Cheng Q, Guengerich FP
Methods Mol Biol. 2013 987: 71-7

PMID: 23475668 · PMCID: PMC3846702 · DOI:10.1007/978-1-62703-321-3_6

Metabolomics provides an invaluable means to interrogate the function of "orphan" enzymes, i.e., those whose endogenous substrates are not known. Here we describe a high-performance liquid chromatography-coupled mass spectrometry (HPLC-MS)-based metabolomics approach to identify an endogenous substrate of an orphan cytochrome P450.

MeSH Terms (7)

Animals Chromatography, High Pressure Liquid Cytochrome P-450 Enzyme System Mass Spectrometry Metabolomics Protein Binding Software

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