Purification of cytochrome P-450 enzymes.

Bell-Parikh LC, Hosea NA, Martin MV, Guengerich FP
Curr Protoc Toxicol. 2002 Chapter 4: Unit4.2

PMID: 23045082 · DOI:10.1002/0471140856.tx0402s12

Among the liver P-450 xenobiotic-metabolizing enzymes, P450-2E1 is of interest because of its activation of potent carcinogens, and P-450 1A2 is of interest because of its role in oxidation of drugs and carcinogens. This unit describes column chromatography protocols for purification of recombinant forms of these enzymes expressed in a bacterial expression system.

MeSH Terms (5)

Chromatography, Liquid Cytochrome P-450 Enzyme System Electrophoresis, Polyacrylamide Gel Humans Isoenzymes

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