Approaches to deorphanization of human and microbial cytochrome P450 enzymes.

Guengerich FP, Tang Z, Cheng Q, Salamanca-Pinzón SG
Biochim Biophys Acta. 2011 1814 (1): 139-45

PMID: 20493973 · PMCID: PMC2939962 · DOI:10.1016/j.bbapap.2010.05.005

One of the general problems in biology today is that we are characterizing genomic sequences much faster than identifying the functions of the gene products, and the same problem exists with cytochromes P450 (P450). One fourth of the human P450s are not well-characterized and therefore considered "orphans." A number of approaches to deorphanization are discussed generally. Several liquid chromatography-mass spectrometry approaches have been applied to some of the human and Streptomyces coelicolor P450s. One current limitation is that too many fatty acid oxidations have been identified and we are probably missing more relevant substrates, possibly due to limits of sensitivity.

Copyright © 2010 Elsevier B.V. All rights reserved.

MeSH Terms (10)

Bacterial Proteins Carcinogens Chromatography, Liquid Cytochrome P-450 Enzyme System Humans Isoenzymes Mass Spectrometry Multigene Family Streptomyces coelicolor Substrate Specificity

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