Purification of cytochromes P450: products of bacterial recombinant expression systems.

Guengerich FP, Martin MV
Methods Mol Biol. 2006 320: 31-7

PMID: 16719372 · DOI:10.1385/1-59259-998-2:31

A general procedure for the solubilization of cytochrome P450 (P450) from bacterial membranes specifically for a human P450 expressed heterologously in the host Escherichia coli is described. The example involves the use of a P450 (3A4) with a C-terminal oligohistidine tag and includes sequential DEAE and metal affinity chromatography.

MeSH Terms (5)

Chromatography, Affinity Chromatography, DEAE-Cellulose Cytochrome P-450 Enzyme System Escherichia coli Recombinant Proteins

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