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EGFR signalling controls cellular fate and pancreatic organogenesis by regulating apicobasal polarity.

Löf-Öhlin ZM, Nyeng P, Bechard ME, Hess K, Bankaitis E, Greiner TU, Ameri J, Wright CV, Semb H
Nat Cell Biol. 2017 19 (11): 1313-1325

PMID: 29058721 · DOI:10.1038/ncb3628

Apicobasal polarity is known to affect epithelial morphogenesis and cell differentiation, but it remains unknown how these processes are mechanistically orchestrated. We find that ligand-specific EGFR signalling via PI(3)K and Rac1 autonomously modulates apicobasal polarity to enforce the sequential control of morphogenesis and cell differentiation. Initially, EGF controls pancreatic tubulogenesis by negatively regulating apical polarity induction. Subsequently, betacellulin, working via inhibition of atypical protein kinase C (aPKC), causes apical domain constriction within neurogenin3 endocrine progenitors, which results in reduced Notch signalling, increased neurogenin3 expression, and β-cell differentiation. Notably, the ligand-specific EGFR output is not driven at the ligand level, but seems to have evolved in response to stage-specific epithelial influences. The EGFR-mediated control of β-cell differentiation via apical polarity is also conserved in human neurogenin3 cells. We provide insight into how ligand-specific EGFR signalling coordinates epithelial morphogenesis and cell differentiation via apical polarity dynamics.

MeSH Terms (17)

Animals Basic Helix-Loop-Helix Transcription Factors Cell Differentiation Cell Polarity Epithelial Cells ErbB Receptors Insulin-Secreting Cells Mice Mice, Knockout Morphogenesis Nerve Tissue Proteins Neuropeptides Organogenesis Phosphatidylinositol 3-Kinases Protein Kinase C rac1 GTP-Binding Protein Signal Transduction

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