Cacna1g is a genetic modifier of epilepsy in a mouse model of Dravet syndrome.

Calhoun JD, Hawkins NA, Zachwieja NJ, Kearney JA
Epilepsia. 2017 58 (8): e111-e115

PMID: 28556246 · PMCID: PMC5554089 · DOI:10.1111/epi.13811

Dravet syndrome, an early onset epileptic encephalopathy, is most often caused by de novo mutation of the neuronal voltage-gated sodium channel gene SCN1A. Mouse models with deletion of Scn1a recapitulate Dravet syndrome phenotypes, including spontaneous generalized tonic-clonic seizures, susceptibility to seizures induced by elevated body temperature, and elevated risk of sudden unexpected death in epilepsy. Importantly, the epilepsy phenotype of Dravet mouse models is highly strain-dependent, suggesting a strong influence of genetic modifiers. We previously identified Cacna1g, encoding the Cav3.1 subunit of the T-type calcium channel family, as an epilepsy modifier in the Scn2a transgenic epilepsy mouse model. In this study, we asked whether transgenic alteration of Cacna1g expression modifies severity of the Scn1a Dravet phenotype. Scn1a mice with decreased Cacna1g expression showed partial amelioration of disease phenotypes with improved survival and reduced spontaneous seizure frequency. However, reduced Cacna1g expression did not alter susceptibility to hyperthermia-induced seizures. Transgenic elevation of Cacna1g expression had no effect on the Scn1a epilepsy phenotype. These results provide support for Cacna1g as a genetic modifier in a mouse model of Dravet syndrome and suggest that Cav3.1 may be a potential molecular target for therapeutic intervention in patients.

Wiley Periodicals, Inc. © 2017 International League Against Epilepsy.

MeSH Terms (13)

Animals Animals, Newborn Calcium Channels, T-Type Disease Models, Animal Electroencephalography Epilepsies, Myoclonic Fever Humans Mice Mice, Inbred C57BL Mutation RNA, Messenger Video Recording

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