Distinct progenitor populations in skeletal muscle are bone marrow derived and exhibit different cell fates during vascular regeneration.

Majka SM, Jackson KA, Kienstra KA, Majesky MW, Goodell MA, Hirschi KK
J Clin Invest. 2003 111 (1): 71-9

PMID: 12511590 · PMCID: PMC151835 · DOI:10.1172/JCI16157

Vascular progenitors were previously isolated from blood and bone marrow; herein, we define the presence, phenotype, potential, and origin of vascular progenitors resident within adult skeletal muscle. Two distinct populations of cells were simultaneously isolated from hindlimb muscle: the side population (SP) of highly purified hematopoietic stem cells and non-SP cells, which do not reconstitute blood. Muscle SP cells were found to be derived from, and replenished by, bone marrow SP cells; however, within the muscle environment, they were phenotypically distinct from marrow SP cells. Non-SP cells were also derived from marrow stem cells and contained progenitors with a mesenchymal phenotype. Muscle SP and non-SP cells were isolated from Rosa26 mice and directly injected into injured muscle of genetically matched recipients. SP cells engrafted into endothelium during vascular regeneration, and non-SP cells engrafted into smooth muscle. Thus, distinct populations of vascular progenitors are resident within skeletal muscle, are derived from bone marrow, and exhibit different cell fates during injury-induced vascular regeneration.

MeSH Terms (17)

Animals Bone Marrow Cells Cell Lineage Cell Separation Endothelium Endothelium, Vascular Flow Cytometry Immunohistochemistry Mice Mice, Inbred C57BL Muscle, Skeletal Myocytes, Smooth Muscle Phenotype Regeneration Reverse Transcriptase Polymerase Chain Reaction Stem Cells Wound Healing

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