The use of fluorescently-tagged apoptolidins in cellular uptake and response studies.

Chong KM, Leelatian N, Deguire SM, Brockman AA, Earl D, Ihrie RA, Irish JM, Bachmann BO, Sulikowski GA
J Antibiot (Tokyo). 2016 69 (4): 327-30

PMID: 26956792 · PMCID: PMC5518790 · DOI:10.1038/ja.2016.22

The apoptolidins are glycomacrolide microbial metabolites reported to be selectively cytotoxic against tumor cells. Using fluorescently tagged active derivatives we demonstrate selective uptake of these four tagged glycomacrolides in cancer cells over healthy human blood cells. We also demonstrate the utility of these five fluorescently tagged glycomacrolides in fluorescent flow cytometry to monitor cellular uptake of the six glycomacrolides and cellular response.

MeSH Terms (8)

A549 Cells Biological Transport Cell Line, Tumor Flow Cytometry Fluorescent Dyes Humans Macrolides Staining and Labeling

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