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Proteolytic surface functionalization enhances in vitro magnetic nanoparticle mobility through extracellular matrix.

Kuhn SJ, Finch SK, Hallahan DE, Giorgio TD
Nano Lett. 2006 6 (2): 306-12

PMID: 16464055 · DOI:10.1021/nl052241g

Steric barriers such as collagen I sharply limit interstitial delivery of macromolecular and nanoparticle (NP) based therapeutic agents. Collagenase-linked superparamagnetic NPs overcame these barriers and moved through in vitro extracellular matrix (ECM) at 90 microm h(-1), a rate similar to invasive cells, under the influence of a magnetic field. NP migration in ECM diminished linearly over 5 days. The collagenase-NP construct overcame two of the most significant barriers to nano- and microscale therapeutics deployment: proteolytic enzyme stability was maintained during a clinically useful time frame by immobilization on the NP surface and degradation of interstitial barriers to tissue biodistribution was enabled by the conjugated microbial protease.

MeSH Terms (11)

Animals Cattle Collagenases Electromagnetic Fields Extracellular Matrix Immobilization In Vitro Techniques Nanostructures Serum Albumin, Bovine Surface Properties Time Factors

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