Cloning of a type I keratin from goldfish optic nerve: differential expression of keratins during regeneration.

Druger RK, Levine EM, Glasgow E, Jones PS, Schechter N
Differentiation. 1992 52 (1): 33-43

PMID: 1283739

We report the cDNA sequence and predicted amino acid sequence of a novel type I keratin, designated as GK50, and show that keratin expression in the goldfish optic nerve is highly complex. The GK50 protein is one of at least three type I keratins expressed in goldfish optic nerve based on both antibody reactivity and blot-binding to the type II keratin ON3. After optic nerve crush in situ hybridization shows a localized increase in GK50 mRNA expression in the crush zone. This is in contrast to ON3 mRNA which shows a localized increase that is limited to the proximal and distal margins of the crush zone, suggesting a diversity of keratin expression in different cell types of the goldfish optic nerve.

MeSH Terms (13)

Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Goldfish Keratins Molecular Sequence Data Nerve Regeneration Optic Nerve Protein Binding RNA, Messenger Sequence Homology, Amino Acid

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