Small-molecule high-throughput screening utilizing Xenopus egg extract.

Broadus MR, Yew PR, Hann SR, Lee E
Methods Mol Biol. 2015 1263: 63-73

PMID: 25618336 · PMCID: PMC4492114 · DOI:10.1007/978-1-4939-2269-7_5

Screens for small-molecule modulators of biological pathways typically utilize cultured cell lines, purified proteins, or, recently, model organisms (e.g., zebrafish, Drosophila, C. elegans). Herein, we describe a method for using Xenopus laevis egg extract, a biologically active and highly tractable cell-free system that recapitulates a legion of complex chemical reactions found in intact cells. Specifically, we focus on the use of a luciferase-based fusion system to identify small-molecule modulators that affect protein turnover.

MeSH Terms (11)

Animals Cell-Free System Drug Evaluation, Preclinical Gene Expression High-Throughput Screening Assays In Vitro Techniques Luciferases Ovum Recombinant Fusion Proteins Small Molecule Libraries Xenopus

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