Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers.

Dorrell C, Grompe MT, Pan FC, Zhong Y, Canaday PS, Shultz LD, Greiner DL, Wright CV, Streeter PR, Grompe M
Mol Cell Endocrinol. 2011 339 (1-2): 144-50

PMID: 21539888 · PMCID: PMC3112273 · DOI:10.1016/j.mce.2011.04.008

Tools permitting the isolation of live pancreatic cell subsets for culture and/or molecular analysis are limited. To address this, we developed a collection of monoclonal antibodies with selective surface labeling of endocrine and exocrine pancreatic cell types. Cell type labeling specificity and cell surface reactivity were validated on mouse pancreatic sections and by gene expression analysis of cells isolated using FACS. Five antibodies which marked populations of particular interest were used to isolate and study viable populations of purified pancreatic ducts, acinar cells, and subsets of acinar cells from whole pancreatic tissue or of alpha or beta cells from isolated mouse islets. Gene expression analysis showed the presence of known endocrine markers in alpha and beta cell populations and revealed that TTR and DPPIV are primarily expressed in alpha cells whereas DGKB and GPM6A have a beta cell specific expression profile.

Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

MeSH Terms (19)

Animals Antibodies, Monoclonal, Murine-Derived Antigens, Surface Cell Separation Diacylglycerol Kinase Dipeptidyl Peptidase 4 Flow Cytometry Glucagon-Secreting Cells Insulin-Secreting Cells Membrane Glycoproteins Mice Mice, Inbred BALB C Nerve Tissue Proteins Pancreas Pancreatic Ducts Prealbumin Rats Rats, Inbred F344 Staining and Labeling

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