Sample Preparation and Analysis of Single Cells Using High Performance MALDI FTICR Mass Spectrometry.

Yang B, Tsui T, Caprioli RM, Norris JL
Methods Mol Biol. 2020 2064: 125-134

PMID: 31565771 · DOI:10.1007/978-1-4939-9831-9_10

Imaging mass spectrometry is a powerful technology that combines the molecular measurements of mass spectrometry with the spatial information inherent to microscopy. This unique combination of capabilities is ideally suited for the analysis of metabolites and lipids from single cells. This chapter describes a methodology for the sample preparation and analysis of single cells using high performance MALDI FTICR MS. Using this approach, we are able to generate profiles of lipid and metabolite expression from single cells that characterize cellular heterogeneity. This approach also enables the detection of variations in the expression profiles of lipids and metabolites induced by chemical stimulation of the cells. These results demonstrate that MALDI IMS provides an insightful view of lipid and metabolite expression useful in the characterization of a number of biological systems at the single cell level.

MeSH Terms (11)

Animals Equipment Design Lipid Metabolism Lipids Metabolome Metabolomics Mice RAW 264.7 Cells Single-Cell Analysis Specimen Handling Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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