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Identification and characterization of an MGSA/GRO pseudogene.

Shattuck-Brandt RL, Wood LD, Richmond A
DNA Seq. 1997 7 (6): 379-86

PMID: 9524820 · DOI:10.3109/10425179709034060

Three linked genes for the CXC-chemokine melanoma growth stimulatory activity/growth related protein (MGSA/GRO) have been previously characterized and mapped to chromosome 4q12-q13. We have isolated and characterized a pseudogene, MGSA/GRO delta, which is 83% similar to the MGSA/GRO alpha gene in the region spanning the 5' UTR, first and second exons, and the first intron. The 5' upstream sequence for the MGSA/GRO delta gene, which is also very similar to the MGSA/GRO alpha, beta, gamma genes, contains a conserved NF-kappa B motif, a TATA box, and a transcription initiation site. However, the sequence becomes markedly divergent after the second exon and hybridization studies indicate that sequences similar to the third and forth exons of other MGSA/GRO genes are not present in this gene. Additional sequence differences include alteration of the MGSA/GRO delta translation initiation codon and a one base insertion resulting in an apparent frame shift and early termination within exon 2. Multiple mutations such as these are characteristic of pseudogenes.

MeSH Terms (17)

Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Chemokine CXCL1 Chemokines, CXC Chemotactic Factors Cloning, Molecular Growth Substances Humans Intercellular Signaling Peptides and Proteins Introns Molecular Sequence Data Pseudogenes Sequence Analysis Sequence Homology, Nucleic Acid Transcription, Genetic

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