Arachidonic acid epoxygenase: structural characterization and quantification of epoxyeicosatrienoates in plasma.

Karara A, Wei S, Spady D, Swift L, Capdevila JH, Falck JR
Biochem Biophys Res Commun. 1992 182 (3): 1320-5

PMID: 1540175 · DOI:10.1016/0006-291x(92)91877-s

Gas chromatographic/mass spectroscopic and chiral analysis showed the presence of enzymatically derived 8,9-, 11,12- and 14,15-EET in rat plasma (2.8:1:3.4 molar ratio, respectively; 10.2 +/- 0.4 ng total EET/ml plasma). Greater than 90% of the plasma EETs was esterified to the phospholipids of circulating lipoproteins. The lipoprotein fraction with the highest EET concentration was LDL (8.1 +/- 0.9 ng/mg of protein) followed by HDL and VLDL (3.5 +/- 0.1 and 1.9 +/- 0.3 ng/mg of protein, respectively). In light of the biological activities of the EETs, these results suggest a potential systemic function for the cytochrome P-450 epoxygenase.

MeSH Terms (13)

8,11,14-Eicosatrienoic Acid Animals Cytochrome P-450 Enzyme System Gas Chromatography-Mass Spectrometry Isomerism Lipoproteins Lipoproteins, HDL Lipoproteins, LDL Lipoproteins, VLDL Male Oxygenases Rats Rats, Inbred Strains

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