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The Transgenic Mouse / ES Cell Shared Resource assists investigators in generating, maintaining and storing germline-altered mice.  This resource, which has been in existence for over 23 years, has generated over 2700 transgenic founder mice from over 750 different DNA constructs and 5000 chimeric mice from over 800 different mouse ES cell clones.  Prior to the discovery of the CRISPR/Cas system, the TMESCSR generated over 160 different gene-targeted mice. Since 2013, the TMESCSR has generated over 400 mutant pups by CRISPR/Cas injections from over 100 different projects.

The Vanderbilt Transgenic Mouse/ES Cell Shared Resource (TMESCSR) provides services, consultation and collaborations to enable the generation, storage and regeneration of genetically altered mice at Vanderbilt.  

We have many years of experience in generating novel transgenic mouse models and are happy to discuss your project with you.  

The following procedures are currently provided on a fee-for-service basis:

We are sorry, but due to low utilization we can no longer provide mouse ES cell-based services on a routine, fee-for-service basis (please see notification letter).    

In addition, we are pleased to discuss collaborative projects that involve the development of new strategies and techniques.  

 

Featured Collections

Publications

Featured publications

  1. Mistargeting of a truncated Na-K-2Cl cotransporter in epithelial cells. Koumangoye R, Omer S, Delpire E (2018) Am J Physiol Cell Physiol
    › Citation · 29719172 (PubMed)
  2. Analysis of neuroanatomical differences in mice with genetically modified serotonin transporters assessed by structural magnetic resonance imaging. Ellegood J, Yee Y, Kerr TM, Muller CL, Blakely RD, Henkelman RM, Veenstra-VanderWeele J, Lerch JP (2018) Mol Autism : 24
    › Citation · 29651330 (PubMed) · PMC5894125 (PubMed Central)
  3. DNPEP is not the only peptidase that produces SPAK fragments in kidney. Koumangoye R, Delpire E (2017) Physiol Rep 5(21)
    › Citation · 29122955 (PubMed) · PMC5688775 (PubMed Central)
  4. A Chimeric Egfr Protein Reporter Mouse Reveals Egfr Localization and Trafficking In Vivo. Yang YP, Ma H, Starchenko A, Huh WJ, Li W, Hickman FE, Zhang Q, Franklin JL, Mortlock DP, Fuhrmann S, Carter BD, Ihrie RA, Coffey RJ (2017) Cell Rep 19(6): 1257-1267
    › Citation · 28494873 (PubMed) · PMC5517093 (PubMed Central)
  5. A Novel HumanMutation Disrupts Dendritic Morphology and Synaptic Transmission, and Causes ASD-Related Behaviors. Stephenson JR, Wang X, Perfitt TL, Parrish WP, Shonesy BC, Marks CR, Mortlock DP, Nakagawa T, Sutcliffe JS, Colbran RJ (2017) J Neurosci 37(8): 2216-2233
    › Citation · 28130356 (PubMed) · PMC5338762 (PubMed Central)
  6. Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation, and Homeostasis by PTF1A. Hoang CQ, Hale MA, Azevedo-Pouly AC, Elsässer HP, Deering TG, Willet SG, Pan FC, Magnuson MA, Wright CV, Swift GH, MacDonald RJ (2016) Mol Cell Biol 36(24): 3033-3047
    › Citation · 27697859 (PubMed) · PMC5126291 (PubMed Central)
  7. Peripheral motor neuropathy is associated with defective kinase regulation of the KCC3 cotransporter. Kahle KT, Flores B, Bharucha-Goebel D, Zhang J, Donkervoort S, Hegde M, Hussain G, Duran D, Liang B, Sun D, Bönnemann CG, Delpire E (2016) Sci Signal 9(439): ra77
    › Citation · 27485015 (PubMed) · PMC5506493 (PubMed Central)
  8. Local effects of human PCSK9 on the atherosclerotic lesion. Giunzioni I, Tavori H, Covarrubias R, Major AS, Ding L, Zhang Y, DeVay RM, Hong L, Fan D, Predazzi IM, Rashid S, Linton MF, Fazio S (2016) J Pathol 238(1): 52-62
    › Citation · 26333678 (PubMed) · PMC5346023 (PubMed Central)
  9. Pro32Pro33 mutations in the integrin β3 PSI domain result in αIIbβ3 priming and enhanced adhesion: reversal of the hypercoagulability phenotype by the Src inhibitor SKI-606. Oliver KH, Jessen T, Crawford EL, Chung CY, Sutcliffe JS, Carneiro AM (2014) Mol Pharmacol 85(6): 921-31
    › Primary publication · 24695082 (PubMed) · PMC4014669 (PubMed Central)
  10. Autism gene variant causes hyperserotonemia, serotonin receptor hypersensitivity, social impairment and repetitive behavior. Veenstra-VanderWeele J, Muller CL, Iwamoto H, Sauer JE, Owens WA, Shah CR, Cohen J, Mannangatti P, Jessen T, Thompson BJ, Ye R, Kerr TM, Carneiro AM, Crawley JN, Sanders-Bush E, McMahon DG, Ramamoorthy S, Daws LC, Sutcliffe JS, Blakely RD (2012) Proc Natl Acad Sci U S A 109(14): 5469-74
    › Citation · 22431635 (PubMed) · PMC3325657 (PubMed Central)

The Transgenic Mouse/ES Cell Shared Resource is supported by NIH grants DK020593 and CA68485.  Please acknowledge this in your publications.

Community Leaders

Contact Information

9410 MRB IV
2213 Garland Avenue
Nashville, TN 37232
United States

Jennifer Skelton
615-936-3454 (p)
615-322-6645 (f)
Email

Keywords & MeSH Terms

MeSH terms are retrieved from PubMed records. Learn more.

Key: MeSH Term Keyword

Adrenergic Uptake Inhibitors CRISPR cryopreservation Diabetes Mellitus, Type 2 Electrophoresis, Polyacrylamide Gel Ephrin-B2 Fluorescent Antibody Technique Fluoxetine Gene Duplication Genes, Immunoglobulin Heavy Chain Insulin Knockout Lipopolysaccharides Lung Neoplasms Membrane Potentials Mice, Knockout Mouse Platelet Endothelial Cell Adhesion Molecule-1 Prosencephalon Receptors, N-Methyl-D-Aspartate rederivation Reflex, Startle Regeneration Reoviridae SOXB1 Transcription Factors Transgenic