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The Transgenic Mouse / ES Cell Shared Resource assists investigators in generating, maintaining and storing germline-altered mice.  This resource, which has been in existence for over 23 years, has generated over 2700 transgenic founder mice from over 750 different DNA constructs and 5000 chimeric mice from over 800 different mouse ES cell clones.  Prior to the discovery of the CRISPR/Cas system, the TMESCSR generated over 160 different gene-targeted mice. Since 2013, the TMESCSR has generated over 400 mutant pups by CRISPR/Cas injections from over 100 different projects.

The Vanderbilt Transgenic Mouse/ES Cell Shared Resource (TMESCSR) provides services, consultation and collaborations to enable the generation, storage and regeneration of genetically altered mice at Vanderbilt.  

We have many years of experience in generating novel transgenic mouse models and are happy to discuss your project with you.  

The following procedures are currently provided on a fee-for-service basis:

We are sorry, but due to low utilization we can no longer provide mouse ES cell-based services on a routine, fee-for-service basis (please see notification letter).    

In addition, we are pleased to discuss collaborative projects that involve the development of new strategies and techniques.  

 

Featured Collections

Publications

Featured publications

  1. Anti-Insulin B Cells Are Poised for Antigen Presentation in Type 1 Diabetes. Felton JL, Maseda D, Bonami RH, Hulbert C, Thomas JW (2018) J Immunol
    › Citation · 29950508 (PubMed)
  2. Mistargeting of a truncated Na-K-2Cl cotransporter in epithelial cells. Koumangoye R, Omer S, Delpire E (2018) Am J Physiol Cell Physiol
    › Citation · 29719172 (PubMed)
  3. p52 expression enhances lung cancer progression. Saxon JA, Yu H, Polosukhin VV, Stathopoulos GT, Gleaves LA, McLoed AG, Massion PP, Yull FE, Zhao Z, Blackwell TS (2018) Sci Rep 8(1): 6078
    › Citation · 29666445 (PubMed) · PMC5904214 (PubMed Central)
  4. Synaptotagmin 4 Regulates Pancreatic β Cell Maturation by Modulating the Ca Sensitivity of Insulin Secretion Vesicles. Huang C, Walker EM, Dadi PK, Hu R, Xu Y, Zhang W, Sanavia T, Mun J, Liu J, Nair GG, Tan HYA, Wang S, Magnuson MA, Stoeckert CJ, Hebrok M, Gannon M, Han W, Stein R, Jacobson DA, Gu G (2018) Dev Cell
    › Citation · 29656931 (PubMed)
  5. Analysis of neuroanatomical differences in mice with genetically modified serotonin transporters assessed by structural magnetic resonance imaging. Ellegood J, Yee Y, Kerr TM, Muller CL, Blakely RD, Henkelman RM, Veenstra-VanderWeele J, Lerch JP (2018) Mol Autism : 24
    › Citation · 29651330 (PubMed) · PMC5894125 (PubMed Central)
  6. NF-κB is weakly activated in the NOD mouse model of type 1 diabetes. Irvin AE, Jhala G, Zhao Y, Blackwell TS, Krishnamurthy B, Thomas HE, Kay TWH (2018) Sci Rep 8(1): 4217
    › Citation · 29523846 (PubMed) · PMC5844878 (PubMed Central)
  7. DNPEP is not the only peptidase that produces SPAK fragments in kidney. Koumangoye R, Delpire E (2017) Physiol Rep 5(21)
    › Citation · 29122955 (PubMed) · PMC5688775 (PubMed Central)
  8. A Chimeric Egfr Protein Reporter Mouse Reveals Egfr Localization and Trafficking In Vivo. Yang YP, Ma H, Starchenko A, Huh WJ, Li W, Hickman FE, Zhang Q, Franklin JL, Mortlock DP, Fuhrmann S, Carter BD, Ihrie RA, Coffey RJ (2017) Cell Rep 19(6): 1257-1267
    › Citation · 28494873 (PubMed) · PMC5517093 (PubMed Central)
  9. A Novel HumanMutation Disrupts Dendritic Morphology and Synaptic Transmission, and Causes ASD-Related Behaviors. Stephenson JR, Wang X, Perfitt TL, Parrish WP, Shonesy BC, Marks CR, Mortlock DP, Nakagawa T, Sutcliffe JS, Colbran RJ (2017) J Neurosci 37(8): 2216-2233
    › Citation · 28130356 (PubMed) · PMC5338762 (PubMed Central)
  10. Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation, and Homeostasis by PTF1A. Hoang CQ, Hale MA, Azevedo-Pouly AC, Elsässer HP, Deering TG, Willet SG, Pan FC, Magnuson MA, Wright CV, Swift GH, MacDonald RJ (2016) Mol Cell Biol 36(24): 3033-3047
    › Citation · 27697859 (PubMed) · PMC5126291 (PubMed Central)

The Transgenic Mouse/ES Cell Shared Resource is supported by NIH grants DK020593 and CA68485.  Please acknowledge this in your publications.

Community Leaders

Contact Information

9410 MRB IV
2213 Garland Avenue
Nashville, TN 37232
United States

Jennifer Skelton
615-936-3454 (p)
615-322-6645 (f)
Email

Keywords & MeSH Terms

MeSH terms are retrieved from PubMed records. Learn more.

Key: MeSH Term Keyword

Adrenergic Uptake Inhibitors CRISPR cryopreservation Diabetes Mellitus, Type 2 Electrophoresis, Polyacrylamide Gel Ephrin-B2 Fluorescent Antibody Technique Fluoxetine Gene Duplication Genes, Immunoglobulin Heavy Chain Insulin Knockout Lipopolysaccharides Lung Neoplasms Membrane Potentials Mice, Knockout Mouse Platelet Endothelial Cell Adhesion Molecule-1 Prosencephalon Receptors, N-Methyl-D-Aspartate rederivation Reflex, Startle Regeneration Reoviridae SOXB1 Transcription Factors Transgenic