The Vanderbilt Genome Editing Resource (VGER) provides services, consultation, and collaborations to enable the generation, storage and regeneration of genetically altered mice at Vanderbilt.  This resource, which was previously called the Transgenic Mouse ESC Shared Resource (please see announcement letter), has been in existence for over 25 years and has generated 200 unique gene-targeted mice between 1993-2015 and more than 100 genome edited mice using CRISPR-Cas9 since 2014.

We have many years of experience in generating novel transgenic mouse models and are happy to discuss your project with you.  

We provide the following services on a fee-for-service basis:

We no longer provide mouse ES cell-based services on a routine, fee-for-service basis (please see notification letter).    

As always, we are pleased to discuss collaborative projects that involve the development of new strategies and techniques.  

Featured Collections

Publications

Featured publications

  1. A smooth muscle-derived, Braf-driven mouse model of gastrointestinal stromal tumor (GIST): evidence for an alternative GIST cell-of-origin. Kondo J, Huh WJ, Franklin JL, Heinrich MC, Rubin BP, Coffey RJ (2020) J Pathol
    › Citation · 32944951 (PubMed)
  2. AMPA Receptor Auxiliary Subunit GSG1L Suppresses Short-Term Facilitation in Corticothalamic Synapses and Determines Seizure Susceptibility. Kamalova A, Futai K, Delpire E, Nakagawa T (2020) Cell Rep 32(3): 107921
    › Citation · 32697982 (PubMed) · PMC7425083 (PubMed Central)
  3. Quantitative Proteomic Analysis of Serotonin Transporter Interactome: Network Impact of the SERT Ala56 Coding Variant. Quinlan MA, Robson MJ, Ye R, Rose KL, Schey KL, Blakely RD (2020) Front Mol Neurosci : 89
    › Citation · 32581705 (PubMed) · PMC7295033 (PubMed Central)
  4. GABA receptor β3 subunit mutation D120N causes Lennox-Gastaut syndrome in knock-in mice. Qu S, Catron M, Zhou C, Janve V, Shen W, Howe RK, Macdonald RL (2020) Brain Commun 2(1): fcaa028
    › Citation · 32467926 (PubMed) · PMC7238755 (PubMed Central)
  5. Excitotoxicity and Overnutrition Additively Impair Metabolic Function and Identity of Pancreatic β-Cells. Osipovich AB, Stancill JS, Cartailler JP, Dudek KD, Magnuson MA (2020) Diabetes 69(7): 1476-1491
    › Citation · 32332159 (PubMed)
  6. Ins1-Cre and Ins1-CreER Gene Replacement Alleles Are Susceptible To Silencing By DNA Hypermethylation. Mosleh E, Ou K, Haemmerle MW, Tembo T, Yuhas A, Carboneau BA, Townsend SE, Bosma KJ, Gannon M, O'Brien RM, Stoffers DA, Golson ML (2020) Endocrinology 161(8)
    › Citation · 32267917 (PubMed) · PMC7354059 (PubMed Central)
  7. Osteopontin and iCD8α Cells Promote Intestinal Intraepithelial Lymphocyte Homeostasis. Nazmi A, Greer MJ, Hoek KL, Piazuelo MB, Weitkamp JH, Olivares-Villagómez D (2020) J Immunol 204(7): 1968-1981
    › Citation · 32102904 (PubMed) · PMC7175606 (PubMed Central)
  8. Identification and Characterization of Unique Neutralizing Antibodies to Mouse EGF Receptor. Jae Huh W, Niitsu H, Carney B, McKinley ET, Houghton JL, Coffey RJ (2020) Gastroenterology 158(5): 1500-1502
    › Citation · 31866246 (PubMed) · PMC7103561 (PubMed Central)
  9. Central EP3 (E Prostanoid 3) Receptors Mediate Salt-Sensitive Hypertension and Immune Activation. Xiao L, Itani HA, do Carmo LS, Carver LS, Breyer RM, Harrison DG (2019) Hypertension 74(6): 1507-1515
    › Citation · 31679420 (PubMed) · PMC7040566 (PubMed Central)
  10. Novel Human NKCC1 Mutations Cause Defects in Goblet Cell Mucus Secretion and Chronic Inflammation. Koumangoye R, Omer S, Kabeer MH, Delpire E (2020) Cell Mol Gastroenterol Hepatol 9(2): 239-255
    › Citation · 31655271 (PubMed) · PMC6957845 (PubMed Central)

VGER, previously the Transgenic Mouse/ES Cell Shared Resource, is supported by NIH grants DK020593 and CA68485.  Please acknowledge this in your publications.

Community Leaders

Contact Information

9410 MRB IV
2213 Garland Avenue
Nashville, TN 37232
United States

Jennifer Skelton
615-936-3454 (p)
615-322-6645 (f)
Email

Keywords & MeSH Terms

MeSH terms are retrieved from PubMed records. Learn more.

Key: MeSH Term Keyword

Adrenergic Uptake Inhibitors CRISPR cryopreservation Diabetes Mellitus, Type 2 Electrophoresis, Polyacrylamide Gel Ephrin-B2 Fluorescent Antibody Technique Fluoxetine Gene Duplication Genes, Immunoglobulin Heavy Chain Insulin Knockout Lipopolysaccharides Lung Neoplasms Membrane Potentials Mice, Knockout Mouse Platelet Endothelial Cell Adhesion Molecule-1 Prosencephalon Receptors, N-Methyl-D-Aspartate rederivation Regeneration Reoviridae SOXB1 Transcription Factors Transgenic Trinucleotide Repeat Expansion