Vanderbilt Genome Editing Resource

The Vanderbilt Genome Editing Resource (VGER) assists investigators in generating, maintaining and storing germline-altered mice in an efficient and cost-effective manner. The VGER provides services, consultation, and collaborations to enable the generation, storage and regeneration of genetically altered mice at Vanderbilt.  This resource, which was previously called the Transgenic Mouse ESC Shared Resource (please see announcement letter), has been in existence for over 25 years and has generated 160 unique gene-targeted mice between 1993-2015 and more than 80 genome edited mice using CRISPR-Cas9 since 2014.

We have many years of experience in generating novel transgenic mouse models and are happy to discuss your project with you.  

We provide the following services on a fee-for-service basis:

We are sorry, but we no longer provide mouse ES cell-based services on a routine, fee-for-service basis (please see notification letter).    

As always, we are pleased to discuss collaborative projects that involve the development of new strategies and techniques.  

Featured Collections

Publications

Featured publications

  1. Central EP3 (E Prostanoid 3) Receptors Mediate Salt-Sensitive Hypertension and Immune Activation. Xiao L, Itani HA, do Carmo LS, Carver LS, Breyer RM, Harrison DG (2019) Hypertension 74(6): 1507-1515
    › Citation · 31679420 (PubMed)
  2. Novel Human NKCC1 Mutations Cause Defects in Goblet Cells Mucus Secretion and Chronic Inflammation. Koumangoye R, Omer S, Kabeer MH, Delpire E (2019) Cell Mol Gastroenterol Hepatol
    › Citation · 31655271 (PubMed)
  3. Histone deacetylase 3 controls a transcriptional network required for B cell maturation. Stengel KR, Bhaskara S, Wang J, Liu Q, Ellis JD, Sampathi S, Hiebert SW (2019) Nucleic Acids Res
    › Citation · 31586401 (PubMed)
  4. Loss of myosin Vb promotes apical bulk endocytosis in neonatal enterocytes. Engevik AC, Kaji I, Postema MM, Faust JJ, Meyer AR, Williams JA, Fitz GN, Tyska MJ, Wilson JM, Goldenring JR (2019) J Cell Biol
    › Citation · 31562230 (PubMed)
  5. p73 regulates epidermal wound healing and induced keratinocyte programming. Beeler JS, Marshall CB, Gonzalez-Ericsson PI, Shaver TM, Santos Guasch GL, Lea ST, Johnson KN, Jin H, Venters BJ, Sanders ME, Pietenpol JA (2019) PLoS One 14(6): e0218458
    › Citation · 31216312 (PubMed) · PMC6583996 (PubMed Central)
  6. The Pdx1-Bound Swi/Snf Chromatin Remodeling Complex Regulates Pancreatic Progenitor Cell Proliferation and Mature Islet β-Cell Function. Spaeth JM, Liu JH, Peters D, Guo M, Osipovich AB, Mohammadi F, Roy N, Bhushan A, Magnuson MA, Hebrok M, Wright CVE, Stein R (2019) Diabetes 68(9): 1806-1818
    › Citation · 31201281 (PubMed) · PMC6702633 (PubMed Central)
  7. Kaiso is required for MTG16-dependent effects on colitis-associated carcinoma. Short SP, Barrett CW, Stengel KR, Revetta FL, Choksi YA, Coburn LA, Lintel MK, McDonough EM, Washington MK, Wilson KT, Prokhortchouk E, Chen X, Hiebert SW, Reynolds AB, Williams CS (2019) Oncogene 38(25): 5091-5106
    › Citation · 30858547 (PubMed) · PMC6586520 (PubMed Central)
  8. Disabling the Gβγ-SNARE interaction disrupts GPCR-mediated presynaptic inhibition, leading to physiological and behavioral phenotypes. Zurawski Z, Thompson Gray AD, Brady LJ, Page B, Church E, Harris NA, Dohn MR, Yim YY, Hyde K, Mortlock DP, Jones CK, Winder DG, Alford S, Hamm HE (2019) Sci Signal 12(569)
    › Citation · 30783011 (PubMed)
  9. p73 Is Required for pIgR Expression in the Respiratory Epithelium. Richmond BW, Du RH, Marshall C, Pietenpol J, Polosukhin VV, Blackwell TS (2018) Ann Am Thorac Soc 15(Supplement_4): S290
    › Citation · 30759000 (PubMed) · PMC6322007 (PubMed Central)
  10. Human Serotonin Transporter Coding Variation Establishes Conformational Bias with Functional Consequences. Quinlan MA, Krout D, Katamish RM, Robson MJ, Nettesheim C, Gresch PJ, Mash DC, Henry LK, Blakely RD (2019) ACS Chem Neurosci 10(7): 3249-3260
    › Citation · 30668912 (PubMed) · PMC6640095 (PubMed Central)

VGER, previously the Transgenic Mouse/ES Cell Shared Resource, is supported by NIH grants DK020593 and CA68485.  Please acknowledge this in your publications.

Community Leaders

Contact Information

9410 MRB IV
2213 Garland Avenue
Nashville, TN 37232
United States

Jennifer Skelton
615-936-3454 (p)
615-322-6645 (f)
Email

Keywords & MeSH Terms

MeSH terms are retrieved from PubMed records. Learn more.

Key: MeSH Term Keyword

Adrenergic Uptake Inhibitors CRISPR cryopreservation Diabetes Mellitus, Type 2 Electrophoresis, Polyacrylamide Gel Ephrin-B2 Fluorescent Antibody Technique Fluoxetine Gene Duplication Genes, Immunoglobulin Heavy Chain Insulin Knockout Lipopolysaccharides Lung Neoplasms Membrane Potentials Mice, Knockout Mouse Platelet Endothelial Cell Adhesion Molecule-1 Prosencephalon Receptors, N-Methyl-D-Aspartate rederivation Regeneration Reoviridae SOXB1 Transcription Factors Transgenic Trinucleotide Repeat Expansion