Vanderbilt Genome Editing Resource

The Vanderbilt Genome Editing Resource (VGER) assists investigators in generating, maintaining, and storing germline-altered mice.  The resource has been in existence for over 27 years and has generated over 2,700 transgenic founder mice from over 700 different DNA constructs and 5,000 chimeric mice from over 800 different mouse ES cell clones to produce over 160 different gene-targeted mice.  Since 2013, VGER has used CRISPR/Cas9 to produce over 140 precise gene mutations in mice for more than 55 investigators.

We have many years of experience in generating novel transgenic mouse models and are happy to discuss your project with you.  

We provide the following services on a fee-for-service basis:

• CRISPR-Cas9 Mouse Editing
• Pronuclear Microinjection of DNAs
• Sperm or Embryo Cryopreservation
• In vitro Fertilization and Rederivation
• Genome-Editing Design and Analysis Services

We no longer provide mouse ES cell-based services on a routine, fee-for-service basis (please see notification letter).    

As always, we are pleased to discuss collaborative projects that involve the development of new strategies and techniques.  

Featured Collections

• Mouse Services
• Prices
• Service Forms
• Technology

Publications

Featured publications

1. A developmental lineage-based gene co-expression network for mouse pancreatic β-cells reveals a role for in pancreas development. Osipovich AB, Dudek KD, Greenfest-Allen E, Cartailler JP, Manduchi E, Potter Case L, Choi E, Chapman AG, Clayton HW, Gu G, Stoeckert CJ, Magnuson MA (2021) Development 148(6)
   › Citation · 33653874 (PubMed)
2. A GRM7 mutation associated with developmental delay reduces mGlu7 expression and produces neurological phenotypes. Fisher NM, AlHashim A, Buch AB, Badivuku H, Samman MM, Weiss KM, Cestero GI, Does MD, Rook JM, Lindsley CW, Conn PJ, Gogliotti RG, Niswender CM (2021) JCI Insight 6(4)
   › Citation · 33476302 (PubMed) · PMC7934925 (PubMed Central)
3. T-B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated Protein. Bonami RH, Nyhoff LE, McNitt DH, Hulbert C, Felton JL, Kendall PL, Thomas JW (2020) J Immunol 205(12): 3263-3276
   › Citation · 33199538 (PubMed) · PMC7725913 (PubMed Central)
4. Neutrophilic inflammation during lung development disrupts elastin assembly and predisposes adult mice to COPD. Benjamin JT, Plosa EJ, Sucre JM, van der Meer R, Dave S, Gutor S, Nichols DS, Gulleman PM, Jetter CS, Han W, Xin M, Dinella PC, Catanzarite A, Kook S, Dolma K, Lal CV, Gaggar A, Blalock JE, Newcomb DC, Richmond BW, Kropski JA, Young LR, Guttentag SH, Blackwell TS (2021) J Clin Invest 131(1)
   › Citation · 33108351 (PubMed) · PMC7773387 (PubMed Central)
5. A smooth muscle-derived, Braf-driven mouse model of gastrointestinal stromal tumor (GIST): evidence for an alternative GIST cell-of-origin. Kondo J, Huh WJ, Franklin JL, Heinrich MC, Rubin BP, Coffey RJ (2020) J Pathol 252(4): 441-450
   › Citation · 32944951 (PubMed) · PMC7802691 (PubMed Central)
6. AMPA Receptor Auxiliary Subunit GSG1L Suppresses Short-Term Facilitation in Corticothalamic Synapses and Determines Seizure Susceptibility. Kamalova A, Futai K, Delpire E, Nakagawa T (2020) Cell Rep 32(3): 107921
   › Citation · 32697982 (PubMed) · PMC7425083 (PubMed Central)
7. Quantitative Proteomic Analysis of Serotonin Transporter Interactome: Network Impact of the SERT Ala56 Coding Variant. Quinlan MA, Robson MJ, Ye R, Rose KL, Schey KL, Blakely RD (2020) Front Mol Neurosci : 89
   › Citation · 32581705 (PubMed) · PMC7295033 (PubMed Central)
8. GABA receptor β3 subunit mutation D120N causes Lennox-Gastaut syndrome in knock-in mice. Qu S, Catron M, Zhou C, Janve V, Shen W, Howe RK, Macdonald RL (2020) Brain Commun 2(1): fcaa028
   › Citation · 32467926 (PubMed) · PMC7238755 (PubMed Central)
9. Excitotoxicity and Overnutrition Additively Impair Metabolic Function and Identity of Pancreatic β-Cells. Osipovich AB, Stancill JS, Cartailler JP, Dudek KD, Magnuson MA (2020) Diabetes 69(7): 1476-1491
   › Citation · 32332159 (PubMed) · PMC7809715 (PubMed Central)
10. Ins1-Cre and Ins1-CreER Gene Replacement Alleles Are Susceptible To Silencing By DNA Hypermethylation. Mosleh E, Ou K, Haemmerle MW, Tembo T, Yuhas A, Carboneau BA, Townsend SE, Bosma KJ, Gannon M, O'Brien RM, Stoffers DA, Golson ML (2020) Endocrinology 161(8)
    › Citation · 32267917 (PubMed) · PMC7354059 (PubMed Central)

VGER, previously the Transgenic Mouse/ES Cell Shared Resource, is supported by NIH grants DK020593 and CA68485.  Please acknowledge this in your publications.

Community Leaders

• Jennifer Skelton
  Manager - VGER
• Mark Magnuson
  Director - VGER
• Leesa Sampson
  Associate Director - VGER
• Pam Uttz
  Administrative Assistant III

Contact Information

9410 MRB IV
2213 Garland Avenue
Nashville, TN 37232
United States

Jennifer Skelton
615-936-3454 (p)
615-322-6645 (f)
Email

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