The Vanderbilt Genome Editing Resource (VGER) assists investigators in generating, maintaining, and storing germline-altered mice.  The resource has been in existence for over 27 years and has generated over 2,700 transgenic founder mice from over 700 different DNA constructs and 5,000 chimeric mice from over 800 different mouse ES cell clones to produce over 160 different gene-targeted mice.  Since 2013, VGER has used CRISPR/Cas9 to produce over 140 precise gene mutations in mice for more than 55 investigators.

We have many years of experience in generating novel transgenic mouse models and are happy to discuss your project with you.  

We provide the following services on a fee-for-service basis:

As always, we are pleased to discuss collaborative projects that involve the development of new strategies and techniques.  

Featured Collections

Publications

Featured publications

  1. Differential regulation of alternate promoter regions in during endodermal and vascular endothelial development. Trinh LT, Osipovich AB, Sampson L, Wong J, Wright CVE, Magnuson MA (2022) iScience 25(9): 104905
    › Citation · 36046192 (PubMed) · PMC9421400 (PubMed Central)
  2. Zn-regulated GTPase metalloprotein activator 1 modulates vertebrate zinc homeostasis. Weiss A, Murdoch CC, Edmonds KA, Jordan MR, Monteith AJ, Perera YR, Rodríguez Nassif AM, Petoletti AM, Beavers WN, Munneke MJ, Drury SL, Krystofiak ES, Thalluri K, Wu H, Kruse ARS, DiMarchi RD, Caprioli RM, Spraggins JM, Chazin WJ, Giedroc DP, Skaar EP (2022) Cell 185(12): 2148-2163.e27
    › Citation · 35584702 (PubMed) · PMC9189065 (PubMed Central)
  3. MTG16 regulates colonic epithelial differentiation, colitis, and tumorigenesis by repressing E protein transcription factors. Brown RE, Jacobse J, Anant SA, Blunt KM, Chen B, Vega PN, Jones CT, Pilat JM, Revetta F, Gorby AH, Stengel KR, Choksi YA, Palin K, Piazuelo MB, Washington MK, Lau KS, Goettel JA, Hiebert SW, Short SP, Williams CS (2022) JCI Insight 7(10)
    › Citation · 35503250 (PubMed) · PMC9220854 (PubMed Central)
  4. Cyclooxygenase-2 in adipose tissue macrophages limits adipose tissue dysfunction in obese mice. Pan Y, Cao S, Tang J, Arroyo JP, Terker AS, Wang Y, Niu A, Fan X, Wang S, Zhang Y, Jiang M, Wasserman DH, Zhang MZ, Harris RC (2022) J Clin Invest 132(9)
    › Citation · 35499079 (PubMed) · PMC9057601 (PubMed Central)
  5. Insm1, Neurod1, and Pax6 promote murine pancreatic endocrine cell development through overlapping yet distinct RNA transcription and splicing programs. Dudek KD, Osipovich AB, Cartailler JP, Gu G, Magnuson MA (2021) G3 (Bethesda) 11(11)
    › Citation · 34534285 (PubMed) · PMC8527475 (PubMed Central)
  6. Altered Ocular Fibrillin Microfibril Composition in Mice With a Glaucoma-Causing Mutation of Adamts10. Wu HJ, Mortlock DP, Kuchtey RW, Kuchtey J (2021) Invest Ophthalmol Vis Sci 62(10): 26
    › Citation · 34424262 (PubMed) · PMC8383930 (PubMed Central)
  7. Targeting diacylglycerol lipase reduces alcohol consumption in preclinical models. Winters ND, Bedse G, Astafyev AA, Patrick TA, Altemus M, Morgan AJ, Mukerjee S, Johnson KD, Mahajan VR, Uddin MJ, Kingsley PJ, Centanni SW, Siciliano CA, Samuels DC, Marnett LJ, Winder DG, Patel S (2021) J Clin Invest
    › Citation · 34292886 (PubMed) · PMC8409586 (PubMed Central)
  8. RNA editing-mediated regulation of calcium-dependent activator protein for secretion (CAPS1) localization and its impact on synaptic transmission. Shumate KM, Tas ST, Kavalali ET, Emeson RB (2021) J Neurochem 158(2): 182-196
    › Citation · 33894004 (PubMed) · PMC8282701 (PubMed Central)
  9. Collagen IV dysfunction in glomerular basement membrane diseases. I. Discovery of a COL4A3 variant in familial Goodpasture's and Alport diseases. Pokidysheva EN, Seeger H, Pedchenko V, Chetyrkin S, Bergmann C, Abrahamson D, Cui ZW, Delpire E, Fervenza FC, Fidler AL, Fogo AB, Gaspert A, Grohmann M, Gross O, Haddad G, Harris RC, Kashtan C, Kitching AR, Lorenzen JM, McAdoo S, Pusey CD, Segelmark M, Simmons A, Voziyan PA, Wagner T, Wüthrich RP, Zhao MH, Boudko SP, Kistler AD, Hudson BG (2021) J Biol Chem : 100590
    › Citation · 33774048 (PubMed) · PMC8100070 (PubMed Central)
  10. A developmental lineage-based gene co-expression network for mouse pancreatic β-cells reveals a role for in pancreas development. Osipovich AB, Dudek KD, Greenfest-Allen E, Cartailler JP, Manduchi E, Potter Case L, Choi E, Chapman AG, Clayton HW, Gu G, Stoeckert CJ, Magnuson MA (2021) Development 148(6)
    › Citation · 33653874 (PubMed) · PMC8015253 (PubMed Central)

VGER, previously the Transgenic Mouse/ES Cell Shared Resource, is supported by NIH grant CA68485.  Please acknowledge this in your publications.

Community Leaders

Contact Information

9410 MRB IV
2213 Garland Avenue
Nashville, TN 37232
United States

Jennifer Skelton
615-936-3454 (p)
615-322-6645 (f)
Email

Keywords & MeSH Terms

MeSH terms are retrieved from PubMed records. Learn more.

Key: MeSH Term Keyword

Adrenergic Uptake Inhibitors CRISPR cryopreservation Ephrin-B2 Fluorescent Antibody Technique Gastrointestinal Neoplasms Interferon-gamma Kidney Cortex Knockout Lipopolysaccharides Lung Neoplasms Moloney murine leukemia virus Mouse Pancreatectomy Platelet Endothelial Cell Adhesion Molecule-1 Principal Component Analysis Programmed Cell Death 1 Receptor Prosencephalon Proto-Oncogene Proteins c-myc Receptors, N-Methyl-D-Aspartate rederivation Reflex, Startle Regeneration Reoviridae SOXB1 Transcription Factors Transgenic