The mission of the Vanderbilt Transgenic Mouse/ES Cell Shared Resource (TMESCSR) is to provide services, consultation and collaboration to enable the generation, storage and regeneration of genetically altered mice at Vanderbilt.  

We have many years of experience in generating novel transgenic mouse models and are happy to discuss your project with you.  

The following procedures are provided on a fee-for-service basis:

  • Crispr/Cas9 mouse technologies
  • Pronuclear microinjection of DNAs
  • Embryo and sperm cryopreservation
  • In vitro fertilization and rederivation

We are sorry, but due to low utilization we can no longer provide mouse ES cell-based services on a routine, fee-for-service basis (please see notification letter).  However, we are happy to discuss how projects that require the genetic manipulation of mouse ES cells might still be achieved.  

In addition, we are pleased to discuss collaborative projects that require the development of new methods and techniques.  

Please email Ms. Jennifer Skelton or Dr. Mark Magnuson with a short description of what you would like to do.

The Transgenic Mouse/ES Cell Shared Resource is supported by NIH grants DK020593 and CA68485.  Please acknowledge this in your publications.


Featured publications

  1. Using a new Lrig1 reporter mouse to assess differences between two Lrig1 antibodies in the intestine. Poulin EJ, Powell AE, Wang Y, Li Y, Franklin JL, Coffey RJ (2014) Stem Cell Res 13(3 Pt A): 422-30
    › Citation · 25460603 (PubMed) · PMC4320017 (PubMed Central)
  2. Dominant and context-specific control of endodermal organ allocation by Ptf1a. Willet SG, Hale MA, Grapin-Botton A, Magnuson MA, MacDonald RJ, Wright CV (2014) Development 141(22): 4385-94
    › Citation · 25371369 (PubMed) · PMC4302917 (PubMed Central)
  3. The rare DAT coding variant Val559 perturbs DA neuron function, changes behavior, and alters in vivo responses to psychostimulants. Mergy MA, Gowrishankar R, Gresch PJ, Gantz SC, Williams J, Davis GL, Wheeler CA, Stanwood GD, Hahn MK, Blakely RD (2014) Proc Natl Acad Sci U S A 111(44): E4779-88
    › Citation · 25331903 (PubMed) · PMC4226116 (PubMed Central)
  4. Attenuated transforming growth factor beta signaling promotes metastasis in a model of HER2 mammary carcinogenesis. Novitskiy SV, Forrester E, Pickup MW, Gorska AE, Chytil A, Aakre M, Polosukhina D, Owens P, Yusupova DR, Zhao Z, Ye F, Shyr Y, Moses HL (2014) Breast Cancer Res 16(5): 425
    › Citation · 25280532 (PubMed) · PMC4303109 (PubMed Central)
  5. Deletion of KCC3 in parvalbumin neurons leads to locomotor deficit in a conditional mouse model of peripheral neuropathy associated with agenesis of the corpus callosum. Ding J, Delpire E (2014) Behav Brain Res : 128-36
    › Citation · 25116249 (PubMed) · PMC4179972 (PubMed Central)
  6. Activated FoxM1 attenuates streptozotocin-mediated β-cell death. Golson ML, Maulis MF, Dunn JC, Poffenberger G, Schug J, Kaestner KH, Gannon MA (2014) Mol Endocrinol 28(9): 1435-47
    › Citation · 25073103 (PubMed) · PMC4154244 (PubMed Central)
  7. Insm1 promotes endocrine cell differentiation by modulating the expression of a network of genes that includes Neurog3 and Ripply3. Osipovich AB, Long Q, Manduchi E, Gangula R, Hipkens SB, Schneider J, Okubo T, Stoeckert CJ, Takada S, Magnuson MA (2014) Development 141(15): 2939-49
    › Citation · 25053427 (PubMed) · PMC4197673 (PubMed Central)
  8. Integrin-mediated type II TGF-β receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling. Chen X, Wang H, Liao HJ, Hu W, Gewin L, Mernaugh G, Zhang S, Zhang ZY, Vega-Montoto L, Vanacore RM, Fässler R, Zent R, Pozzi A (2014) J Clin Invest 124(8): 3295-310
    › Citation · 24983314 (PubMed) · PMC4109532 (PubMed Central)
  9. ETO family protein Mtg16 regulates the balance of dendritic cell subsets by repressing Id2. Ghosh HS, Ceribelli M, Matos I, Lazarovici A, Bussemaker HJ, Lasorella A, Hiebert SW, Liu K, Staudt LM, Reizis B (2014) J Exp Med 211(8): 1623-35
    › Citation · 24980046 (PubMed) · PMC4113936 (PubMed Central)
  10. Inducible loss of one Apc allele in Lrig1-expressing progenitor cells results in multiple distal colonic tumors with features of familial adenomatous polyposis. Powell AE, Vlacich G, Zhao ZY, McKinley ET, Washington MK, Manning HC, Coffey RJ (2014) Am J Physiol Gastrointest Liver Physiol 307(1): G16-23
    › Citation · 24833705 (PubMed) · PMC4080164 (PubMed Central)

Community Leaders

Contact Information

9410 MRB IV
2213 Garland Avenue
Nashville, TN 37232
United States

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Keywords & MeSH Terms

MeSH terms are retrieved from PubMed records. Learn more.

Key: MeSH Term Keyword

Adrenergic Uptake Inhibitors CRISPR cryopreservation Diabetes Mellitus, Type 2 Electrophoresis, Polyacrylamide Gel Ephrin-B2 Fluorescent Antibody Technique Fluoxetine Gene Duplication Genes, Immunoglobulin Heavy Chain Immunoblotting Insulin Knockout Lipopolysaccharides Lung Neoplasms Membrane Potentials Mice, Knockout Moloney murine leukemia virus Mouse Pancreatectomy Receptors, N-Methyl-D-Aspartate rederivation Reflex, Startle Regeneration SOXB1 Transcription Factors Transgenic