The Vanderbilt Genome Editing Resource (VGER) assists investigators in generating, maintaining and storing germline-altered mice in an efficient and cost-effective manner. The VGER provides services, consultation, and collaborations to enable the generation, storage and regeneration of genetically altered mice at Vanderbilt.  This resource, which was previously called the Transgenic Mouse ESC Shared Resource (please see announcement letter), has been in existence for over 25 years and has generated 160 unique gene-targeted mice between 1993-2015 and more than 70 genome edited mice using CRISPR-Cas9 since 2014.

We have many years of experience in generating novel transgenic mouse models and are happy to discuss your project with you.  

We provide the following services on a fee-for-service basis:

We are sorry, but we no longer provide mouse ES cell-based services on a routine, fee-for-service basis (please see notification letter).    

As always, we are pleased to discuss collaborative projects that involve the development of new strategies and techniques.  

Featured Collections

Publications

Featured publications

  1. Neurog3-Independent Methylation Is the Earliest Detectable Mark Distinguishing Pancreatic Progenitor Identity. Liu J, Banerjee A, Herring CA, Attalla J, Hu R, Xu Y, Shao Q, Simmons AJ, Dadi PK, Wang S, Jacobson DA, Liu B, Hodges E, Lau KS, Gu G (2019) Dev Cell 48(1): 49-63.e7
    › Citation · 30620902 (PubMed) · PMC6327977 (PubMed Central)
  2. Active Kras Expression in Gastric Isthmal Progenitor Cells Induces Foveolar Hyperplasia but Not Metaplasia. Choi E, Means AL, Coffey RJ, Goldenring JR (2019) Cell Mol Gastroenterol Hepatol 7(1): 251-253.e1
    › Citation · 30585163 (PubMed) · PMC6305842 (PubMed Central)
  3. Transgene-associated human growth hormone expression in pancreatic β-cells impairs identification of sex-based gene expression differences. Stancill JS, Osipovich AB, Cartailler JP, Magnuson MA (2019) Am J Physiol Endocrinol Metab 316(2): E196-E209
    › Citation · 30532991 (PubMed)
  4. Loss of MYO5B Leads to Reductions in Na Absorption With Maintenance of CFTR-Dependent Cl Secretion in Enterocytes. Engevik AC, Kaji I, Engevik MA, Meyer AR, Weis VG, Goldstein A, Hess MW, Müller T, Koepsell H, Dudeja PK, Tyska M, Huber LA, Shub MD, Ameen N, Goldenring JR (2018) Gastroenterology 155(6): 1883-1897.e10
    › Citation · 30144427 (PubMed) · PMC6279525 (PubMed Central)
  5. Mistargeting of a truncated Na-K-2Cl cotransporter in epithelial cells. Koumangoye R, Omer S, Delpire E (2018) Am J Physiol Cell Physiol 315(2): C258-C276
    › Citation · 29719172 (PubMed) · PMC6139505 (PubMed Central)
  6. p52 expression enhances lung cancer progression. Saxon JA, Yu H, Polosukhin VV, Stathopoulos GT, Gleaves LA, McLoed AG, Massion PP, Yull FE, Zhao Z, Blackwell TS (2018) Sci Rep 8(1): 6078
    › Citation · 29666445 (PubMed) · PMC5904214 (PubMed Central)
  7. Synaptotagmin 4 Regulates Pancreatic β Cell Maturation by Modulating the Ca Sensitivity of Insulin Secretion Vesicles. Huang C, Walker EM, Dadi PK, Hu R, Xu Y, Zhang W, Sanavia T, Mun J, Liu J, Nair GG, Tan HYA, Wang S, Magnuson MA, Stoeckert CJ, Hebrok M, Gannon M, Han W, Stein R, Jacobson DA, Gu G (2018) Dev Cell 45(3): 347-361.e5
    › Citation · 29656931 (PubMed) · PMC5962294 (PubMed Central)
  8. Analysis of neuroanatomical differences in mice with genetically modified serotonin transporters assessed by structural magnetic resonance imaging. Ellegood J, Yee Y, Kerr TM, Muller CL, Blakely RD, Henkelman RM, Veenstra-VanderWeele J, Lerch JP (2018) Mol Autism : 24
    › Citation · 29651330 (PubMed) · PMC5894125 (PubMed Central)
  9. B Cell-Intrinsic mTORC1 Promotes Germinal Center-Defining Transcription Factor Gene Expression, Somatic Hypermutation, and Memory B Cell Generation in Humoral Immunity. Raybuck AL, Cho SH, Li J, Rogers MC, Lee K, Williams CL, Shlomchik M, Thomas JW, Chen J, Williams JV, Boothby MR (2018) J Immunol 200(8): 2627-2639
    › Citation · 29531165 (PubMed) · PMC5893413 (PubMed Central)
  10. NF-κB is weakly activated in the NOD mouse model of type 1 diabetes. Irvin AE, Jhala G, Zhao Y, Blackwell TS, Krishnamurthy B, Thomas HE, Kay TWH (2018) Sci Rep 8(1): 4217
    › Citation · 29523846 (PubMed) · PMC5844878 (PubMed Central)

VGER, previously the Transgenic Mouse/ES Cell Shared Resource, is supported by NIH grants DK020593 and CA68485.  Please acknowledge this in your publications.

Community Leaders

Contact Information

9410 MRB IV
2213 Garland Avenue
Nashville, TN 37232
United States

Jennifer Skelton
615-936-3454 (p)
615-322-6645 (f)
Email

Keywords & MeSH Terms

MeSH terms are retrieved from PubMed records. Learn more.

Key: MeSH Term Keyword

Adrenergic Uptake Inhibitors CRISPR cryopreservation Diabetes Mellitus, Type 2 Electrophoresis, Polyacrylamide Gel Ephrin-B2 Fluorescent Antibody Technique Fluoxetine Gene Duplication Genes, Immunoglobulin Heavy Chain Insulin Knockout Lipopolysaccharides Lung Neoplasms Membrane Potentials Mice, Knockout Mouse Platelet Endothelial Cell Adhesion Molecule-1 Prosencephalon Receptors, N-Methyl-D-Aspartate rederivation Regeneration Reoviridae SOXB1 Transcription Factors Transgenic Trinucleotide Repeat Expansion