The mission of the Vanderbilt Transgenic Mouse/ES Cell Shared Resource (TMESCSR) is to provide services, consultation and collaboration to enable the generation, storage and regeneration of genetically altered mice at Vanderbilt.  

We have many years of experience in generating novel transgenic mouse models and are happy to discuss your project with you.  

The following procedures are provided on a fee-for-service basis:

  • Crispr/Cas9 mouse technologies
  • Pronuclear microinjection of DNAs
  • Embryo and sperm cryopreservation
  • In vitro fertilization and rederivation

We are sorry, but due to low utilization we can no longer provide mouse ES cell-based services on a routine, fee-for-service basis (please see notification letter).  However, we are happy to discuss how projects that require the genetic manipulation of mouse ES cells might still be achieved.  

In addition, we are pleased to discuss collaborative projects that require the development of new methods and techniques.  

Please email Ms. Jennifer Skelton or Dr. Mark Magnuson with a short description of what you would like to do.

The Transgenic Mouse/ES Cell Shared Resource is supported by NIH grants DK020593 and CA68485.  Please acknowledge this in your publications.

Publications

Most recent publications

  1. The PGE2 EP3 Receptor Regulates Diet-Induced Adiposity in Male Mice. Ceddia RP, Lee D, Maulis MF, Carboneau BA, Threadgill DW, Poffenberger G, Milne G, Boyd KL, Powers AC, McGuinness OP, Gannon M, Breyer RM (2016) Endocrinology 157(1): 220-32
    › Citation · 26485614 (PubMed)
  2. NF-κB-dependent airway inflammation triggers systemic insulin resistance. Cyphert TJ, Morris RT, House LM, Barnes TM, Otero YF, Barham WJ, Hunt RP, Zaynagetdinov R, Yull FE, Blackwell TS, McGuinness OP (2015) Am J Physiol Regul Integr Comp Physiol 309(9): R1144-52
    › Citation · 26377563 (PubMed) · PMC4666958 (PubMed Central)
  3. Macrophages are essential for CTGF-mediated adult β-cell proliferation after injury. Riley KG, Pasek RC, Maulis MF, Dunn JC, Bolus WR, Kendall PL, Hasty AH, Gannon M (2015) Mol Metab 4(8): 584-91
    › Citation · 26266091 (PubMed) · PMC4529497 (PubMed Central)
  4. Activation of FoxM1 Revitalizes the Replicative Potential of Aged β-Cells in Male Mice and Enhances Insulin Secretion. Golson ML, Dunn JC, Maulis MF, Dadi PK, Osipovich AB, Magnuson MA, Jacobson DA, Gannon M (2015) Diabetes 64(11): 3829-38
    › Citation · 26251404 (PubMed) · PMC4613976 (PubMed Central)
  5. The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma. Krah NM, De La O JP, Swift GH, Hoang CQ, Willet SG, Chen Pan F, Cash GM, Bronner MP, Wright CV, MacDonald RJ, Murtaugh LC (2015) Elife
    › Citation · 26151762 (PubMed) · PMC4536747 (PubMed Central)
  6. Reversing Tolerance in Isotype Switch-Competent Anti-Insulin B Lymphocytes. Williams JM, Bonami RH, Hulbert C, Thomas JW (2015) J Immunol 195(3): 853-64
    › Citation · 26109644 (PubMed) · PMC4506889 (PubMed Central)
  7. Bruton's Tyrosine Kinase Synergizes with Notch2 To Govern Marginal Zone B Cells in Nonobese Diabetic Mice. Case JB, Bonami RH, Nyhoff LE, Steinberg HE, Sullivan AM, Kendall PL (2015) J Immunol 195(1): 61-70
    › Citation · 26034172 (PubMed) · PMC4551465 (PubMed Central)
  8. Targeted inhibition of ANKRD1 disrupts sarcomeric ERK-GATA4 signal transduction and abrogates phenylephrine-induced cardiomyocyte hypertrophy. Zhong L, Chiusa M, Cadar AG, Lin A, Samaras S, Davidson JM, Lim CC (2015) Cardiovasc Res 106(2): 261-71
    › Citation · 25770146 (PubMed) · PMC4481572 (PubMed Central)
  9. Aldosterone modulates thiazide-sensitive sodium chloride cotransporter abundance via DUSP6-mediated ERK1/2 signaling pathway. Feng X, Zhang Y, Shao N, Wang Y, Zhuang Z, Wu P, Lee MJ, Liu Y, Wang X, Zhuang J, Delpire E, Gu D, Cai H (2015) Am J Physiol Renal Physiol 308(10): F1119-27
    › Citation · 25761881 (PubMed) · PMC4437001 (PubMed Central)
  10. SPAK-mediated NCC regulation in response to low-K+ diet. Wade JB, Liu J, Coleman R, Grimm PR, Delpire E, Welling PA (2015) Am J Physiol Renal Physiol 308(8): F923-31
    › Citation · 25651563 (PubMed) · PMC4398835 (PubMed Central)

Community Leaders

Contact Information

9410 MRB IV
2213 Garland Avenue
Nashville, TN 37232
United States

No contact person provided

Keywords & MeSH Terms

MeSH terms are retrieved from PubMed records. Learn more.

Key: MeSH Term Keyword

Adrenergic Uptake Inhibitors CRISPR cryopreservation Diabetes Mellitus, Type 2 Electrophoresis, Polyacrylamide Gel Ephrin-B2 Fluorescent Antibody Technique Fluoxetine Gene Duplication Genes, Immunoglobulin Heavy Chain Immunoblotting Insulin Knockout Lipopolysaccharides Lung Neoplasms Membrane Potentials Mice, Knockout Moloney murine leukemia virus Mouse Pancreatectomy Receptors, N-Methyl-D-Aspartate rederivation Reflex, Startle Regeneration SOXB1 Transcription Factors Transgenic