The focus of our work is to understand how the reparative function of resident lung mesenchymal stem cells (MSC) is altered during the development and course of lung diseases including pulmonary hypertension, fibrosis and emphysema. In order to define a role for lung MSC in the context of lung disease and their regulation by the microenvironment we employ mouse models. Additionally we use patient derived induced pluripotent stem cells (iPS) as a cell based model to understand molecular changes in which occur in mesenchyme and vascular cell populations as a result of disease specific gene mutations.
Our laboratory has identified and characterized lung MSC and demonstrated that these cells are present in the distal lung associated with the microvasculature in both mouse and human tissue. Recent studies in our lab have shown that proper function of the lung MSC is that of an adult angioblast which plays a role in maintaining vascular integrity. Loss of proper MSC function results in loss of tissue integrity and function.
There is an increasing emphasis on the development of cell-based therapies to address these conditions, but the lung is a recalcitrant candidate for these strategies because of the diverse cell types and functions as well as a lack of understanding of how chronic disease processes affect stem cell differentiation. Therefore, prior to testing cell-based therapy, it is desirable to use pre-clinical models of lung injury and chronic disease to determine how changes in the lung tissue during the development of disease affect resident stem cell differentiation and function.
1161 21st Ave. S.
Nashville, TN 37232
No contact person provided
MeSH terms are retrieved from PubMed records. Learn more.
Key: MeSH Term KeywordATP-Binding Cassette Transporters Bone Marrow Cells Cell Differentiation Cell Lineage Endothelium, Vascular Flow Cytometry Hematopoietic Stem Cells Humans induced pluripotent stem cells lung mesenchymal stem cells Mesenchymal Stem Cells Mice, Inbred C57BL Molecular Probes Multipotent Stem Cells Muscle, Skeletal Myofibroblasts Pericytes Phenotype pulmonary Reverse Transcriptase Polymerase Chain Reaction Stem Cell Transplantation Transforming Growth Factor beta1 Wound Healing