Our laboratory currently has two major NIH funded research interests:
1) We are studying the innate mechanisms regulating progenitor cell expansion and tissue regeneration following acute kidney injury, and evaluating the use of small molecules identified from a high content screen in Zebrafish embryos for their ability to accelerate these innate mechanisms of tissue regeneration following injury. To this end we are developing a number of assays and tools to evaluate renal epithelial cell regenerative capacity in vivo, and are actively exploring the role of chromatin in regulating these regenerative events.
2) Our lab is also studying the role of BMP signaling in regulating reactivity and remodeling of the lung vasculature both in normal individuals, and in patients and mice carrying a number of different heritable mutations in the BMP type 2 receptor (BMPR2) locus. To do this we are developing mouse genetic models, reporters and along with Kevin Ess and Aaron Bowman, optimizing reprogramming conditions to develop inducible pluripotent stem cells (IPSCs) from patients with hereditary pulmonary arterial hypertension (HPAH). We are also testing a variety of different protocols to promote efficient endothelial and smooth muscle cell differentiation of IPSCs in order to study the biology of BMP signaling defects in the vasculature of patients with HPAH.
Other interests in the laboratory include the development of a genetic model of Wilms’ tumorgenesis in mice, and the analysis of signaling pathways governing asymmetric organ growth in mice with late gestational placental insufficiency.
- Mark de Caestecker
Associate Professor of Medicine
1161 21st Ave. S.
Nashville, TN 37232
No contact person provided
MeSH terms are retrieved from PubMed records. Learn more.
Key: MeSH Term KeywordBAC transgenic mice BMP signaling breast cancer cell adhesion COS Cells Cross-Linking Reagents Cytokines Familial Primary Pulmonary Hypertension Fibroblast Growth Factors Imidazoles Indoles In Situ Hybridization lineage tracing Membrane Proteins Mice, SCID multipotent epithelial progenitor cells phosphorylation Proteinuria Pulmonary Artery pulmonary hypertension Reagent Strips Receptor, Transforming Growth Factor-beta Type II Receptor Protein-Tyrosine Kinases renal development Saccharomyces cerevisiae Single-Blind Method Smad2 Protein Thiazoles Tissue Distribution transcription factor vascular remodeling Wilms tumor